53 years old female showed repeated ulceration of labial gingival mucosa at upper and lower anterior teeth, which was a partly desquamated and erythematous lesion. The lesion was slightly extended into vestibule and buccal mucosa in oral cavity, but the similar lesion was not found in other organs by medical inspection. The incisional biopsy including the border of the ulcerated mucosa and normal mucosa showed a severely inflamed mucosa, of which epithelium was gradually detached from the underlying conective tissue, so that it was diagnosed as a mucous membrane pemphigoid (MMP) pathologically. The epithelium was thinned, almost lost its rete pegs, and the basement membrane was completely distorted by the epithelial detachement. The inflammatory cell infiltration was mainly composed of small round cells and plasma cells. Immunohistochemistry was performed to know the expression of pathogenetic proteins using antisera of Igk, E-cadherin, laminin a5, elafin, and eIF5A. The basement membrane at the epithelial detachment was condensely positive for Igk, and the involved epithelium became atrophic but showed consistently positive reaction of matrix proteins and protein translation factor, i.e., E-cadherin, laminin a5, elafin, and eIF5A similar to the adjacent normal mucosa continuous to the MMP lesion. The Igk was also diffusely deposited on the basement membrane of nearby normal mucosa. Many plasma cells infiltrated around the lesion were strongly positive for Igk in their cytoplasms. Therefore, we suggest that the MMP be characterized by the deposition of Igk on the basement membrane of the detached epithelium in the absence of no other pathognomic changes of molecular events.
thus, resembles scar tissue. TGF-β1, MMP and TIMP play an essential role in remodeling extracellular matrix during scar formation. This study investigates the pathogenesis of IF with respect to the coordinated expression of factors involved in wound healing. Proliferative activity and expression of TGF-β1, MMP-1 and TIMP-1 were observed using immunohistochemistry in 88 cases of IF and 9 cases of normal oral mucosa(NOM). Proliferative activity and expression of TGF-β1 and TIMP-1 were increased in IF compared to NOM. MMP-1 expression was not significantly increased in IF. We propose that IF is caused by increased expression of TGF-β1 and an imbalance in expression of MMP-1 and TIMP-1.
Tumor cells under hypoxic conditions are often found due to the rapid outgrowth of their vascular supply, and,in order to survive hypoxia, these cells induce numerous signaling factors. Erk is an important kinase in cell survival, and its activity is regulated by Raf kinases through numerous growth factor receptors. The authors investigated Erk activation and Raf/Erk signaling using the hypoxia-mimetic agent, cobalt chloride (CoCl2), in oral squamous cell carcinoma (OSCC) cells. CoCl2 increases Erk phosphorylation in both a dose- and time-dependent manner. In addition, blocking the activation of epidermal growth factor receptor (EGFR) using PD168393 abolished Erk activation in response to CoCl2, suggesting that Erk phosphorylation by CoCl2 is dependent on EGFR.
Artemisia scoparia (A. scoparia), perennial herb is indigenous to Korea and has been traditionally used in liver damage. We investigated the effect of the essential oil obtained from A. scoparia on apoptosis of KB cells. Cytotoxicity and cellular DNA content were analyzed by MTT assay and flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. We found that the essential oil induced the apoptosis of the KB cells by concentrations of 0.4 to 0.2 mg/ml which was verified by DNA fragmentation, apoptotic bodies, and the sub-G0/G1 ratio. The essential oil also transient caspase-9 and caspase-3 activity and cleavage of PARP in KB cells for 24 h. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. In conclusion, we demonstrated that the essential oil of A. scoparia induces apoptosis in KB cells
was first described by Pindborg in 1955. they occur as intraosseous(94%) and extraosseous variants. Although the prognosis of CEOT was regarded as ameloblastoma in the past, contemporary accumulating data suggest that CEOT have better prognosis than ameloblastoma. But decisive evidences are lacked. Although CEOT is a rare odontogenic tumor, the histopathologic features have so much diversity. Especially interesting aspects are the being of amyloid and Langerhans' cells. Author classify 6 cases of CEOT to scanty, small, and lage as produced amount of amyloid and then perform immunohistochemical study about pancytokertin, cytokeratin8/18, vimentin, CD1a, and VEGF(vascular endothelial growth factor) for verifying the differentiation state of tumor cells and the comparative infiltrative potential with ameloblastoma. Author obtain several conclusion and presumptive facts through this study and previous researchs. Tumor cells of CEOT exhibited different differentiating features as amount of amyloid, presumably tumor cells of CEOT with scanty amount of amyloid represent enamel epithelium-like cells of presecretory stage in amelogenesis, tumor cells of CEOT with small amount of amyloid represent ameloblast-like cells of secretory stage in amelogenesis, and tumor cells of CEOT with large amount of amyloid represent reduced enamel epithelium-like cells after enamel formation. Epithelial-Mesenchymal transition phenomenon developed in tumor cells of CEOT with small amount of amyloid. Inflammatory reaction was not related with being Langerhansʼ cells. Author tentatively concluded that CEOT with Langerhans cells exhibited a tendency of non-calcification, scanty amyloid formation and frequently occurring at the maxillary anterior region through the previous studies and this study. Infiltrative growth potential of CEOT was lower than ameloblastoma regarding only VEGF expression.
29-year-old female with chronic renal failure and a history of hemodialysis during 10 years showed localized jaw enlargement in the anterior mandible. She also revealed increased serum PTH level, osteosclerosis in her skull base and facial bone, chronic pain in the both knee joint, and gastrointestinal disorder which are symptoms of renal osteodystrophy. The patient was diagnosed hyperparathyroidism associated with chronic renal failure. Parathyroidectomy was performed, however, serum PTH level increased again after the parathyroidectomy, the affected area of the mandible was enlarged as the serum PTH level increased. The enlarged area seemed like as peripheral lesion clinically. We reported a case of localized jaw enlargement as oral manifestation in renal osteodystrophy.