The role of CXCR7, a seven-transmembrane G-protein coupled chemokine receptor, which binds with high affinity to chemokine CXCL11 and CXCL12 in oral cancer cells and the effect of transient CXCR7-downregulation on proliferation and migration of oral squamous cell carcinoma (OSCC) cells have not been reported. The aim of the present study was to evaluate the effects of CXCR7 on an OSCC cell line. In this study, we down-regulated CXCR7 in the KOSCC25B OSCC cell line by siRNA. In vitro cell proliferation and migration assays were used to investigate the effect of CXCR7- downregulation on cell proliferation and migration in si.KOSCC25B cells. The CXCR7 down-regulated OSCC cells grew significantly slower than the negative control siRNA transfected KOSCC25B cells (p<0.05). Additionally, migration of si.KOSCC25B cells decreased significantly compared with non-transfected KOSCC25B cells (p<0.007). These results suggest that down-regulation of CXCR7 induces anti-proliferative and anti-migratory effects in OSCC, and that CXCR7 may be a useful target molecule for the treatment of OSCC.
As human's average lifespan is gradually increased, the interest in the quality of life and health is also increasing. More and more customers like to use the facial manual technique as a way to live a healthy life, instead of using it as measures to simply improve skin troubles or to have beautiful skin. The facial manual technique performed in the skin care stage is closely related to the decrease of stress. In order to verify the effects of facial manual technique on the decrease of stress, an experiment was conducted in the same method by dividing 16 women residing in Seoul into four groups, from September 1st to September 30th 2016. To understand their general characteristics, perceptual stress, and satisfaction with facial manual technique through questionnaire, the frequency analysis and descriptive statistics were performed by using SPSS 22.0 program. The paired t-test was conducted to see changes in stress, LF, HF, LF/HF, average pulse, pulse standard, and average deviation before/after experiment. The stress index was decreased in every group. Especially, the group of the 20s showed a huge decrease(24.3%), which was significant to every group except for the 50s. In the results of LF, the group of the 20s and of the 30s increased 2.8% and 4.6% respectively, which was significant. In the group of the 40s, it was increased 4.0% while it was decreased 1.2% in the 50s. Rather than women in climacteric age, it was more effective for women in younger age. In results of pulse standard, it was increased 36.5% in the 20s, 63.6% in the 40s respectively, which was significant(p<0.05, p<0.01). In the group of the 30s and 50s, the significant values were not shown although it was still increased 48.3% and 65.1% respectively. In the survey of the facial manual technique, the whole participants showed positive responses to its effects on the decrease of stress. A majority of them took easy to control feelings, and it was highly possible for them to use it perio-dically in the future. It suggested that the effects of facial manual technique could play a role on recovering psychological stability and decrease of stress. It could be considered as a safe and effective healthcare method to improve the quality of human's life.
Hyaluronic acid is natural polysaccharide existing around skin or cartilage, and is known as a moisturizing factor. It is expected that hyaluronic acid cosmetics market will continue to grow in the future, as its use and demand increases, like medical supplies and health food besides cosmetics containing hyaluronic acid. However, There are few present studies on content in cosmetics containing hyaluronic acid. The purpose of this study was to evaluate satisfaction and examine what effect was produced on sebum and moisture in skin after applying to cosmetics the hyaluronic acid helpful in maintaining moisture in skin at 5%, 20% and 50% concentration. For 71 days, a clinical trial in a total 39 persons living in the national capital region was carried out. And sebum and moisture was measured through skin diagnosis system A-ONE TAB before trial and each day when clinical trial was conducted for hyaluronic acid content group. Besides, this study was to examine subjects' general characteristics, eating habits, and living habits, and satisfaction after the clinical trial. This study analyzed a difference according to the time to measure the skin condition among hyaluronic acid content groups by utilizing statistical package program SPSS v. 21.0 through data coding and data cleaning process. There was no statistically significant difference in hyaluronic acid content according to any of demographic characteristics, eating habits and living habits among groups (p>.05). With regard to hyaluronic acid content, there was no statistically significant difference in moisture and sebum prior to clinical trial in T zone and U zone among groups (p>.05). A change of moisture in T zone among groups according to hyaluronic acid content showed statistically significant difference in the 2nd week (F =3.636, p<.05). And the highest level was shown in 50%(44.15±2.672), being followed by 20%(43.15±2.340), and 5%(41.62±2.219). Accordingly, it was shown that, as hyaluronic acid content relatively increased, the moisture in T zone increased. In the results of analyzing the change of moisture in U zone, the change of sebum in T zone and U zone by group according to hyaluronic acid content, no statistically significant difference was shown in sebum level of T zone by group(p>.05). For analyzing the subjective satisfaction after clinical trial among groups according to hyaluronic acid content, satisfaction score was the highest in 'skin moistness' (4.38±0.650) for 5% group, 'skin moistness' (4.69±0.480) for 20% group and 'skin texture smoothness' (4.54±0.519) for 50% group. And no statistically significant difference was shown in each subjective satisfaction by group according to hyaluronic acid content(p>.05). It was thought that the results of skin condition and subjective satisfaction survey according to hyaluronic acid content would be baseline data for developing cosmetics containing the hyaluronic acid and marketing a skin moisturizer.
Osteoblastoma(OB) is a rare tumor of bone representing less than 1 % of all tumors of the maxillofacial region. The lesion usually appears in vertebral column, sacrum, long bones but rare in mandible or maxilla. The lesion occurs most commonly at age from 3 to 78 years with the mean age of 22-23 years. In this article, we report one case of OB occurred in mandible. With clinical examination and radiological diagnosis, preliminary diagnosis was made as Fibro-osseous lesion, osteomyelitis or cementoblastoma. Under general anesthesia, associated tooth was extracted and excisional biopsy was done. After microscopic examination, it was diagnosed as OB. The patients which we presented did not complain any specific complications, and showed good prognosis.