Immunoprecipitation-based high performance liquid chromatography (IP-HPLC) is a type of modified enzyme-linked immunosorbent assay (ELISA) that uses protein A/G (or antibody)-conjugated beads instead of the antibody-conjugated wells used in ELISA. In order to determine the fidelity of IP-HPLC, the author used 83 antisera to identify protein expression changes caused by cisplatin treatment in KB human oral cancer cells. KB cells were cultured for 12 or 24 hours with 10 ug/mL cisplatin. The results obtained by IP-HPLC were comparable with published cisplatin data, although ELISA was not conducted in the present study. Cisplatin dominantly reduced the levels of proteins associated with cell proliferation, transcription factors, growth factors, cytoskeletal proteins, and cellular differentiating factors, but on the other hand, apoptosis-related factors, oncogenes, and protective proteins were usually up-regulated, presumably to address cisplatin-induced DNA damage. In particular, cisplatin directly inactivated genomic DNA by down-regulating histone H1 and demethylase and by up-regulating deacetylase. Cisplatin also rapidly induced p53 overexpression and mitochondria-mediated endogenous apoptosis occurred after 12 hours of cisplatin treatment, although this was almost completely replaced by FASL/FAS-mediated exogenous apoptosis after 24 hours. This preliminary study was conducted to investigate the anticancer effect of cisplatin on the KB human oral cancer cells and to determine the fidelity of IP-HPLC data. It was concluded that IP-HPLC is useful for identifying profile changes of genome wide essential proteins and signaling changes of major molecular pathways.
Hippo signaling is one of the signal transduction pathways revealed in Drosophila and mammalian. This signaling is known to control proliferation and growth of normal cells or cancer cells, in which many signaling proteins form a network. In this network, tumor suppressor kinases include MST and LATS while YAP and TAZ exist as oncoproteins. Combined with transcription factor, YAP the oncoprotien starts to secrete growth factors such as CTGF, FGF, and Cry61 regulating the growth of cells or the organ sizes. The YAP is also associated with the development of early embryo and the regeneration of the skin wound as well as abnormal growth of cancers in case of over-expression. Although many previous studies have found various tumors with YAP over-expressed, the expression of YAP is not yet clearly identified in oral cancer.
The aim of this study was to check the expression level of YAP in oral squamous cell carcinoma. So we performed PCR and Western blot to check YAP expression in mRNA and protein level respectively. In result, all of the 13 cell lines examined has presented the expression of YAP, and especially in HSC2 and KOSCC11 cell lines has been observed the remarkable level of expression.
In conclusion, we confirmed the overexpression of YAP cell line in oral squamous cell carcinoma, it will be a great help to the study carried out in the future. Once you understand the mechanism of oncoprotien YAP in oral cancer cells, it seems possible to research and development of targeted tumor therapy agents in oral cancer.
Objective: The aim of this study was to analyze factors indicating preventive removal of mandibular third molars by determining associated symptoms, pathologies, eruption state, position and angulation types of mandibular third molars.
Study design: A retrospective study was made of 436 patients(200 females, 236 males), aged between 12 and 81 years (mean: 29.93 years) undergoing panoramic radiographic examinations. Total 700 mandibular third molars were analyzed. They were divided by age, sex, position and angulation of mandibular third molars, bony coverage, and associated pathologies- caries, pericoronitis, periodontitis, cyst, root resorption of adjacent teeth.
Results: Among 409 pathologies associated with mandibular third molars, pericoronitis accounted for 34.2%(140 cases), which was the most common lesion, caries in the second and third molars for 28.9%, caries in the second molars for 11%(45 cases). Periodontitis showed in 7%(29 cases). In 4 cases, root resorption of adjecent tooth showed. The position which showed predominant pathologic lesion according to the Pell and Gregory classification was ⅡA(86.5%), followed by ⅡB(71.6%). Mandibular third molars without bony coverage(64.8%) showed pathologies frequently.
Conclusions: The removal of mandibular third molars must be determined by the perceived risk if the teeth are not removed. The position and inclination of the third molars, bony coverage, age and sex of patients can be the important evidence in the decision making process.
Fibromatoses are benign fibroblastic proliferation, which forms infiltrating tumor-like mass. Their histologic features cannot reflect the biologic behavior; local recurrence and aggressive infiltration to the adjacent tissue make fibromatoses intermediate tumor, between benign and malignant. Infantile fibromatosis consists of desmoid-type tumor and lipofibromatosis, and mainly affects children under age of 10. While desmoid-type tumor is characterized by elongated mature fibroblastic proliferation, the fibroblasts in lipofibromatosis look more primitive and accompany adipocytes. Here, we report a case of infantile fibromatosis of 4 month old boy involving the tongue.
Subcutaneous emphysema is a rare but serious side effect of dental and oral surgery procedures. The condition is characterized by air being forced underneath the tissue, leading to swelling, crepitus on palpation, and with potential to spread along the fascial planes to the periorbital, mediastinal, pericardial, and/or thoracic spaces. A wide range of causes have been documented for the origin of subcutaneous emphysema during dental treatment including: crown preparations, other operative procedures, endodontic therapy, extractions, as well as oral surgery procedures.
The patient was a 58-year-old woman who presented to the Seoul Saint mary’s hospital emergency department with a chief complaint of facial edema, dyspnea and chest discomfort after periodontal treatment using an air-flow equipment in local dental clinic. During treatment in the emergency department, oxygen therapy and intravenous injection of steroid and anti-histamine was done. it was noted that the patient had pain and swelling on left lower molar region, pus discharging on same site. Severe edema was observed on periorbital region to neck with heatness. An audible crepitus sound was heard during palpation on facial area. Neck CT scan and antibiotic therapy was done, as symptom suggesting dental abscess is observed. 3 hours after injection of antibiotics, the patient’s symptom was relieved, but she felt chest discomfort continuously. CT scan with constrast depicted confluent and extensive soft tissue emphysematous changes involving face and deep neck spaces and pneumomediastinum. The patient was refered to thoracic surgery department, oxygen therapy was decided continuously. After 10 hours, patient’s chief complaint was resolved, and discharged. After 1 week, all symptom was disappear and follow-up neck CT scan finding was disappearance of edema and pnuemomediastinum.
We report a case of cervical subcutaneous emphysema and pneumomediastinum occurring after periodontal treatment using an air-flow equipment and case on the diagnosis and treatment of subcutaneous emphysema and pneumomediastinum, along with a review of the literature.