Elevated expression of survivin is strongly associated with tumorigenesis and even in human common cancers. The purpose of this study is to confirm whether survivin is associated with odontogenic tumor expecially in the development and growth in ameloblastomas. For the control group; 3 specimens obtained from normal oral mucosa without any inflammatory reaction were used. For the experimental group, specimens obtained from 17 subjects of ameloblastomas; follicular type, plexiform type, granular cell type, acantomatous type and unicystic ameloblastoma. All the specimens were embedded in paraffin, sectioned 5μm or more in thickness, and stained with hematoxylin- eosin stain method. For immunostain, the specimens were incubated with 1:200 diluted primary antibody, followed by the secondary antibody. The bound antibodies were visualized by addition of diaminobenzidine tetrahydrochloride (DAB) for 30 minutes at room temperature. The specimens were counterstained with Gill’s Hematoxylin and mounted. Intensity of survivin immunoreactivity specimens was quantitatively scaled using under the light microscope with the following criteria; Intensive reaction; +++, Moderate reaction; ++, Minimal reaction; +. Using the image analyzer (Korea Optical System), immunoreactivity of the tumor cells in various fields was measured and statistically analyzed with SPSS 17.0 Program. In control group, moderate positive reaction was noted in the cytoplasm of cells in the basal and spinous layer, but negative reaction was revealed in the nucleus. Expression of survivin was significantly increased in the cytoplasm of ameloblastomas as compared to that of control group (p<0.05). Expression of survivin in the nucleus and the cytoplasm of the tumor cells between subtype of ameloblastoma was not significantly different. These results suggest that expression of survivin is closely associated with the development, and growth of the ameloblastomas. However it is unlikely that survivin can be used as a marker for cellular malignancy.
Because of the irreversible nature of periodontal disease, early diagnosis is an important aspect of management of patients with periodontal disease. Human saliva is an attractive medium for disease diagnosis because its collection is noninvasive and simple. Analysis of saliva may be especially beneficial in the determination of current periodontal status and serve as means for the screening of periodontal disease. In the present study, we investigated potential biochemical markers in whole saliva samples for the screening of periodontal disease using proteomics technique. We enrolled five subjects each from four different groups on the basis of measures of periodontal health (healthy group, gingivitis group, chronic periodontitis group and aggressive periodontitis group). Eleven proteins in whole saliva samples were identified as differentially expressed proteins between the healthy and periodontal disease groups using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight / time-of-flight mass spectrophotometry (MADLI-TOF/TOF MS) approaches. Although the diagnostic value of oral fluid has been recognized for some time and potential biomarkers of periodontal disease have been identified in saliva, this, to our knowledge, is one of the first studies to examine large-scale proteomic profiling to identify the extent of periodontal destruction. Thus, this work provides an important framework for future efforts aimed at understanding salivary responses to periodontal destruction and predicting the future disease progression.
Oral squamous cell carcinoma (OSCC) has been a focus of cancer prevention studies due to the fact that it occurs by a multistep process and that a precancerous lesion in the oral mucosa is easily accessible. The present study was aimed at developing an optical detection system using autofluorescence spectrum measurements for the early detection of oral cancer. The optical detection system was designed to use an excitation wavelength of 337 nm emanating from a Xenon lamp. Precancerous and cancerous lesions were created in the hamster buccal pouch by treatment with 7,12-dimethylbenz[a]anthracene (DMBA). Four groups of five hamsters each were used in this experiment. The right buccal pouch was treated with 0.5% DMBA to induce carcinogenesis while the left buccal pouch was treated with mineral oil as a control. The autofluorescence of both buccal pouches was measured weekly. A difference in the excitation pattern between the normal and the carcinogen-treated tissue was noticed after three weeks. Specifically, the intensity of the autofluorescence spectrum in the DMBA-treated buccal pouch was increased at wavelengths between 400 and 450 nm. The results of the autofluorescence measurements were compared to histological findings and show that the intensity of the autofluorescence increased along with the stage of epithelial dysplasia. Based on the fact that one of the autofluorophores in this tissue is NADH, we measured the fluorescence at the 450-nm NADH wavelength to conclude that the increased autofluorescence in the dysplastic areas may be caused by NADH. Based on these data, we suggest that autofluorescence optical methods are a useful tool for the early detection of oral cancer.
Urokinase-type plasminogen activator (uPA) and plasminogen activator type 1 (PAI-1) inhibitor contribute to the invasiveness of many carcinomas. It will be helpful to study clinical behavior of patients with malignant tumor by analysis of their expression. Expression of uPA and PAI-1 in human salivary gland tumors has been rarely reported in vitro. The purpose of this study were to investigate the protein expression of uPA and PAI-1 in SGT cell line compared to oral SCC and HeLa cell lines and to study migration and adhesion assay. All the cell lines were cultured under DMEM with 10% FBS at at 37oC in a 5% CO2 incubator. We studied a possible association between cytosolic uPA and PA-1 concentrations in SGT cell line compared to any other cell lines through cell migration and adhesion assay, and enzyme-linked immunoassay(ELISA). In migration assay SGT cell line was about 2 .5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPA cytosolic concentrations of SGT cell line was about 3-10 folds, while PAI-1 was about 2.5-10 folds. Oral SCC cell lines were the lowest value. Both uPA and PAI-1 concentrations were correlated with migration and adhesion assay. High cytosolic concentrations of uPA and PAI-1 was correlated with migration and adhesion assay. It suggested that these markers might be specific marker for SGT cell line and would be contributed to treatment and prognosis of human salivary gland adenocarcinoma
The origin of squamous cell components in salivary gland tumor has been not yet clarified in detail. The squamous cell differentiation from adenocarcinoma has been reported in various carcinoma by HPV transfection in vitro. The adenocarcinoma cells adjacent to the squamous cell carcinoma components were positive for HPV. This is thought to indicate that after adenocarcinoma cells are transfected with HPV, they undergo morphological changes, and that squamous cell differentiation follows. The purpose of this study were to examine the effects of HPV-16 E6/E7 gene transfection into SGT cell line from human salivary gland adenocarcinoma, and to study the relation between the E6/E7 gene and squamous differentiation. Plasmid pBR322 containing HPV-16 was transfected into cultured SGT cell line using lipofectin method. Hygromycin was used as a selection marker. The presence of HPV E6/E7, transglutaminase 1, and involucrin mRNAs and protein in E6/E7 gene transfected cells was investigated by RT-PCR and immunoslot blot method. The apoptosis index was analysed by flow cytometry. The growth rate of E6/E7 gene transfected cells was reduced. E6/E7 transfected SGT cells increased apoptosis index. Involucrin and TGase I mRNAs by the squamous cell differentiation was most conspicuous in the E6/E7 gene transfected cell compared with non transfected cells. Squamous cell differentiation demonstrated in the transfectedSGT cell line, which expressed E6/E7 fusion gene mRNA.E6/E7 gene transfected cells showed squamous cell differentiation, expressing involucrin and TGase 1 protein by immunoslot blotting. The transfected SGT cell which expressed E6/E7 gene mRNA showed the squamous cell differentiation particularly clearly, and apoptosis was also demonstrated. It suggested that E6/E7 gene transfection into human salivary gland adenocarcinoma cells might induce clear squamous cell differentiation and contribute to study the pathogenesis of human salivary gland adenocarcinoma.
Benign fibrous histiocytomas that is also known asDermatofibroma,Fibrous dermatofibroma, andFibrous histiocytoma are benign skin growths. They are composed of disordered collagen laid down by fibroblasts. In rare cases, basal cell carcinoma may develop in that. Benign fibrous histiocytomas of bone are unusual neoplasms that often are confused with metaphyseal fibrous defects. It is an uncommon neoplasm of the Head and Neck region. It is a rare and usually painless oral tumor. Several cases were reported in mandible, but few in maxilla, especially in maxillary gingiva. We are reporting a case of Maxillay gingival.