간행물
대한구강악안면병리학회지 KCI 등재 The Korean Journal of Oral and Maxillofacial Pathology
- 발행기관 대한구강악안면병리학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1225-1577 (Print)2384-0900 (Online)
- 수록기간 1977 ~ 2024
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
제30권 제6호 (2006년 12월) 6건
1.
2006.12
구독 인증기관 무료, 개인회원 유료
Combined epithelial odontogenic tumors are very rare and represent hybrid lesion comprising adenomatoid odontogenic tumor intermixed with calcifying epithelial odontogenic tumor. The authors present 3 cases of combined epithelial odontogenic tumor which contained diagnostic areas for both adenomatoid odontogenic tumor and calcifying epithelial odontogenic tumor. Their behaviour and histogenesis were discussed.
4,000원
2.
2006.12
구독 인증기관 무료, 개인회원 유료
The purpose of this study was to evaluate the role of c-fos and c-jun expression in the odontogenic cysts. For this study, 20 subjects of odontoenic dysts: 8 subjects of keratocysts and 12 subjects of periodontal cysts referred to the Dept. of Oral Pathology, School of Dentistry, Kyung Hee University, were used as experimental group, and 5 subjects of normal oral mucosa without any inflammatory changes. were used as control groups respectively. All the tissues of experimental and control group were fixed in neutral formalin solution and embedded in paraffin, serial tissue section were made 5㎛ in thickness and processed in the standard way for immunohistochemical method, using primary antibodies, for c-fos, c-jun was diluted at 1:100 each, followed by the super sensitive non-biotin horse radish peroxidase system with DAB as chromogen application, counter stained with Gill's hematoxylin stainmethod, mounted. And examined under the biologic microscope with the criteria of -(no epithelial stain), +(weak or focal epithelial stain), ++(moderate or focal intensive epithelial stain), +++(intense generalized epithelial staining) for the epithelial, and stromal tissues on each. Attained results as follows. In normal oral mucosa, it is noted that moderate positive responses in cytoplasm and nuclei to c-fos and c-jun protein on each.. In the responses to c-fos, c-jun protein, moderate positive responses in nuclei and cytoplasms of the epithelial lining in keratocysts and periodontal cysts, but more intense reaction is noted on the periodontal cyst compare to that on the keratocysts. In the responses to c-fos, c-jun, it is noted that more intense responses in odontogenic cyst compare to that in the oral mucosa. In the responses to c-fos and c-jun on submucoas of oral normal mucosa, focal epithelial stain was noted. and more intensive reaction was noted on the odontogenic cysts, most in periodontal cysts. This results suggests that c-fos and c-jun protein effected on the induction of development and growth of the cysts
4,000원
3.
2006.12
구독 인증기관 무료, 개인회원 유료
Gemcitabine (Gemzar, 2,2-difluorodeoxycytidine, or dFdC) is an analog of cytosine arabinoside with anti-tumor activity in a few human cancers (lung, ovary, pancreatic and breast cancers). However, the mechanism of apoptosis by this compound in carcinoma has not been fully elucidated. In the present study, we investigated that gemcitabine alone and combination with cisplatin or 5-FU are cancer toxicity using lung cancer cell line A549 by MTT, FACS analysis, and Western blot assay. Also, to confirm enhanced antitumor activity in vivo using an xenograft tumor model. The MTT assay showed higher cytotoxic effect in combination with gemcitabine-cisplatin or gemcitabine-5-FU than gemcitabine alone. FACS analysis showed that gemcitabine-cisplatin combination increased hypodiploid DNA to 70.84 %. The induction of apoptosis showed more increase in combination with gemcitabine-cisplatin or gemcitabine-5-FU than gemcitabine alone. The Western results showed higher expression of p53 and p21WAF/CIP1 protein in combination treatment with gemcitabine-cisplatin or gemcitabine-5-FU than gemcitabine treatment alone. But, Bcl-2 protein expression decreased. In vivo experiments showed that more decreased tumor size and more increased survival rate on combination with gemcitabine- cisplatin or gemcitabine-5-FU combination than gemcitabine alone. In conclusion, this study suggests that gemcitabine combined with cisplatin or 5-FU are the synergistic effect of anticancer therapy on lung cancer.
4,000원
4.
2006.12
구독 인증기관 무료, 개인회원 유료
Extensive oral mucosa loss from a variety of conditions is associated with significant functional morbidity and mortality. Although it is known that keratinocytes are a rich source of wound healing promoting factors such as transforming growth factor-β1(TGF-β1), it is not clear whether differentiated keratinocytes in a multi-layer form release this multi-functional growth factor. This study examined the hypothesis that keratinocytes in mono- and multi-layer forms expressed different levels of TGF-β1. When NHOK reached confluency in serum free medium(KBM), in test medium containing 1.2 mM Ca++ KBM NHOK were allowed to form multi-layers and differentiate. The purpose of this study were to investigate the mRNA level of TGF-β1, FGF-2, and TIMP-1 by RT-PCR analysis and also to evaluate the expression of TGF-β1 and involucrin in keratinocytes at different times of the onset of differentiation. The numbers and sizes of these nodules were increased as the process of keratinocyte differentiation proceed. Cultured NHOK in preconfluency under KBM medium expressed a significantly higher level of TGF-β1 relative to those grown in multi-layer forms, while the level of TGF-β1 mRNA gradually reduced to its lowest level at 7 days of growing cells in test medium. Cultured NHOK in preconfluency of KBM medium expressed a lower level of FGF-2 and TIMP-1 relative to those grown in multi-layer forms, while the level of FGF-2 and TIMP-1 mRNA showed the highest level at 3 days at gradually reduced to its lowest level at 7 days of growing cells in test medium. As a differentiation marker for keratinocytes at different time points, the highest level of involucrin mRNA expression was found at the later stage of cell differentiation. It suggested that the expression of TGF-β1 mRNA be consistent with the expression of FGF-2 and TIMP-1 mRNA in NHOK grown in high calcium medium during the terminal differentiation. But differentiated NHOK expressing higher involucrin mRNA could show constant espression of TGF-β1, FGF-2 and TIMP-1.
4,000원
5.
2006.12
구독 인증기관 무료, 개인회원 유료
The purpose of this study was to observe the histopathologic tissue reaction in vital bone in applying the various treated implants. For this purpose, twelve New Zealand Albino rats, weighing 3.3 to 4 kg were used as experimental animals. All the experimental groups divided into four groups; Machined surface as control, RBM(resorbable blast media), Hydroxyapatite-sand and Porous coating groups. All the experimental implants were examined under the scannning electron microscope. All the experimental rabbits were implanted in the tibial metaphyses of rabbits under the general anesthesia with Ketamine HCl(2.5ML /kg.body wt.) injections. For prevention of infection after implant, prophylactic erythromycine injections, 250mg/body wt.(Aldrich Co. USA) were performed on each. On the sixth week after implant, all the experimental rabbits were sacrificed with over dose of Sedaject(Samwoo Pharm .Co. Korea). All the tissues containing each experimental materials were fixed in ethyle alcohol, and embedded in spurr resin(Polytechnic Co. USA) as usual manner. sectioned in 12 um thickness, grinded , stained with the Vulenueva's osteochrome stain methed and examined histopatholgically. For measuring the distances between the implant and bone without any connective tissue interface, all the distances were calculated the length of the implant direct contact to bones. using the view analyzer program( Korea Optical Co.) and the statistical analysis were performed using the one-way ANOVA test. The statistical differences were considered significant below 5% level. Following results were obtained. On the scanning electron microscopic examination, dull cracked continuous linear indentations were revealed on the machined surface implant, irregular sharp indentation on the resorbabale blast, irregular thin exophytic or indentated leaflets on the hydroxyapatite-sand implant, and long ovoid globular particles were revealed on the porous coating implant surface respectively. On the histopathologic examination, complete osseointegation was noted between the implant and cortex bone on the collar and the apex lesion and in parts, small newley formed bone spicules attached to the screws in marrow tissues with compatibility in all experimental groups, but on the aspect of the tissue compatibility to the various implant materials, the superiority of the materials could not identified. The ratio of drect contact between the bone and implant, the HA sand gorup was the most superior among the gorups and followed by the machine surface, but on RBM and porous coating groups were inferior compared to that on the experimental groups. With these results, the superiority of tissue compatibility among the experimental implant group could not be identified
4,000원
6.
2006.12
구독 인증기관 무료, 개인회원 유료
The production of insoluble glucan was affected by the concentration of ions and buffers in a mouth. In this study, the effects of ions and buffers on the mRNA expression of gtfB and gtfC gene of Streptococcus mutans which is an important causative agent of dental caries were investigated by Fluorescent in situ hybridization(FISH). The mRNA of gtfB and gtfC gene was normally expressed in the BHI broth containing 1 % sucrose. The gtfB and gtfC mRNA expression was increased at 0.25 mM and 4 mM of CaCl2, respectively. While the mRNA expression of two genes was inhibited at each concentration of KCl and MgCl2 when compared to the control. As for buffers, the gtfB and gtfC mRNA expression was lower than the control by the addition of sodium bicarbonate. The addition of sodium phosphate decreased the gtfB mRNA expression except for 100 mM in which the expression was increased, and the gtfC mRNA expression was similar to or lower than the control at each concentraion of sodium phosphate. The mRNA expression of the gtfB and gtfC was decreased at each concentration of potassium phosphate when compared to the control.
4,000원
