간행물
대한구강악안면병리학회지 KCI 등재 The Korean Journal of Oral and Maxillofacial Pathology
- 발행기관 대한구강악안면병리학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1225-1577 (Print)2384-0900 (Online)
- 수록기간 1977 ~ 2024
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
제27권 제1호 (2003년 3월) 6건
1.
2003.03
구독 인증기관 무료, 개인회원 유료
It has been said that amino acid transporters play an important role in supplying nutrition to normal and cancer cells and for cell proliferation. System L is a major nutrient transport system responsible for the Na+-independent transport of large neutral amino acids including several essential amino acids. In malignant tumors, a system L transporter L-type amino acid transporter 1(LAT1) is up-regulated to support tumor cell growth. In the present study, we have examined the function of LAT1 and its expression in the KB human oral epidermoid carcinoma cells. RT-PCR, western blot analysis and immunohistochemical analysis have revealed that KB cells express LAT1 in the plasma membrane together with its associating protein 4F2hc, whereas KB cells do not express the other system L isoform LAT2. The uptake of L-[14C]leucine by KB cells is Na+-independent and almost completely inhibited by system L selective inhibitor BCH. The profiles of the inhibition of L-[14C]leucine uptake by amino acids in the KB cells are comparable with those for the LAT1 expressed in Xenopus oocytes. The majority of L-[14C]leucine uptake is, therefore, mediated by LAT1 in the KB cells. These results suggest that the uptakes of neutral amino acids including several essential amino acids in the KB oral epidermoid carcinoma cells mediated by LAT1. In addition, specific inhibition of LAT1 by such agents as BCH in human oral squamous cell carcinomas will be a new rationale for anti-cancer therapy.
4,500원
2.
2003.03
구독 인증기관 무료, 개인회원 유료
Odontogenic keratocysts(OKCs) are frequently associated with erupted or impacted tooth. In such instances, the radiographic features simulate those of a dentigerous cyst. The purpose of this study was to evaluate a comparative immunohistochemical expression of Ki-67 as a proliferative marker and apoptotic signals in the OKC associated with or without impacted tooth. In addition, we have also been investigated with regard to the proliferative activity and apoptosis comparing the unilocular and multilocular varieties of the OKC. The material for this study consisted of thirty-two cases of OKCs (OKC with impacted tooth, n=16；OKC without impacted tooth, n=16) and ten cases of dentigerous cysts as a comparison. The results revealed that the proliferative activity and apoptosis of OKCs with impacted tooth was higher than those of dentigerous cysts. However, there was no correlation between Ki-67 immunoreactivity or apoptosis and association with or without impacted tooth in 32 cases of OKCs. In addition, this present study showed that there was no correlation between the unilocular and multilocular varieties of the OKCs in proliferative activity and apoptosis.
4,000원
3.
2003.03
구독 인증기관 무료, 개인회원 유료
The imbalance between epithelial cell growth and inhibitory factors may cause human epithelial cancer. The dysregulation of growth inhibitory effect of TGF-β1 has been recognized in a variety of carcinomas. This study aimed to investigate the expression of TGF-β1 type II receptors(TβR-II) in the carcinogenesis of oral squamous cell carcinoma(OSCC). Six cell lines established from OSCC in the department of Oral Pathology, Yonsei University College of Dentistry were used. DNA was extracted from harvested cells by phenol-chloroform method. Polymerase chain reaction (PCR) was done with each primer of exon 3, 4, 5, 6 of TβR-II gene. PCR products were inserted to cloning vector (pGEM-T easy vector) and then analyzed to automatic DNA sequencing analyzer. Reverse transcriptase-PCR (RT-PCR) was performed to confirm the mRNA expression of TβR-II gene. Western blotting was performed to detect the expression of the TβR-II protein. As results, a frameshift within a polyadenine region of exon 3 was found in YD-8 cell line. In YD-17 cell line, a missense mutation at codon 238 of exon 4 was found, suggesting the alteration of amino acid from asparagine to aspartic acid. TβR-II mRNA was detected in all cancer cell lines, but it was slightly decreased as compared to that of normal oral mucosal cells. In Western blotting, no TβR-Ⅱ protein was detected in all OSCC cell lines. These results suggested that the altered regulation of TGF-β1 function might play a role in the development of OSCC.
4,200원
4.
2003.03
구독 인증기관 무료, 개인회원 유료
Young Joon Lee, Sang Shin Lee, Byoung Geol Park, Yeon Sook Kim, Suk Keun Lee, Hyock Soo Moon, Je Geun Chi
The primary growth center (MdPGC) of human fetal mandible was conspicuously distinguished in the soft X-ray view of fetal mandibles.1) As the peripheral adaptive growth of mandible advanced during the postnatal period, the MdPGC became overshadowed by condensed cortical bone. However, in the well-processed radiograms of adult mandible a condensed radiopaque image, measuring 0.5-1.0 cm in diameter, can be observed below the apex of first premolar. In this study we aimed to trace a sclerotic sequela of mandibular primary growth center during postnatal period. Panoramic radiograms of two hundreds adults and soft X-ray views of thirty dry mandible were analyzed by statistical methods. The adult MdPGC was clearly distinguishable from the mental foramen. The area of MdPGC was seldom changed in the older persons, even in the edentulous mandibles. Additionally, the benign lesions of odontogenic cysts and tumors hardly destroyed the original structure of MdPGC, while the malignant tumors of squamous cell carcinoma and metastatic cancer rapidly destroyed and resolved the radiopaque area of the MdPGC.
4,000원
5.
2003.03
구독 인증기관 무료, 개인회원 유료
Normal development of human fetal teeth during prenatal period play an important role in analyzing abnormal teeth formation and examining pathologic approach to abnormal teeth formation. The purposes of this study were to describe the normal development of dental hard tissues in fetal teeth compared to abnormal teeth formation through review and literature. We will apply these study to examine the pathologic alterations of human teeth in the future.
3,000원
6.
2003.03
구독 인증기관 무료, 개인회원 유료
Hydroxyapatite(HA) has been widely used as bone substitutes to rehabilitate bone loss area by new bone formation. But there were some problem of bone formation around HAs due to a little space between HAs embedded in bone loss area. The purpose of this study was to observe morphologically new bone formation around HAs mixed with PLGA block (5.5㎜ in diameter, 4 mm in depth) in Newzealand white rabbit tibia. Before 1 week of sacrification, Alizarin red was injected intraperitoneally into rabbit. At 3 day, 1, 2, 4, 10, 18, 32 weeks, bones with HA as control group(CG)and HA mixed with PLGA block as experimental group (EG)were fixed with 10 % neutral formalin, dehydrated, and embedded with Spurr low viscosity resin. After the specimens were cut by 500 ㎛ with slow diamond wheel saw, these were coated with carbon and examined by REM, LSM and qualifative analysis of calcium and phosphorous deposition were done with EPMA. The obtained results were as follows. 1. Both group showed scattered HA around compact bone under REM and little AZ labelled bone under LSM at 3 days. 2. Both group showed active AZ labelled bone, while EG showed higher Ca(calcium) and P(phosphate) deposition than that of CG at 1 wk. 3. There was decreased AZ labelled bone of both group under LSM. REM of EG showed HA associated with new bone from compact bone, while EPMA features showed similar to Ca and P deposition at 1 wk and EG showed higher than that of CG. 4. REM features of both group showed resorbed HA associated with new bone. There was decreased AZ labelled bone of both group under LSM. EPMA features showed higher Ca and P deposition at 4 wks than that of 2 wks. 5. New bone of both group was well demarcated from compact bone under REM at 10 wks. LSM features showed various AZ labelled bone, but weak AZ labelled than that of CG. 6. LSM features of 14 wks showed discontinuous AZ labelled on osteon formation. EPMA of both group showed increased Ca deposition, while there was higher Ca deposition of EG and similar P deposition to CG. 7. REM featureless of 18 wks in EG showed similar gray color to compact bone. LSM features showed osteon formation with little concentric lamellars. EPMA of both group showed increased Ca deposition, while there was higher Ca and P deposition of EG. 8. New bone was ill demarcated from compact bone and increased otseon formation of REM features at 32 wks. There was little AZ labelled bone. EPMA features showed higher Ca and P deposition of EG than that of compact bone. From the aboving results, there was early active AZ labelled bone of both group within 1-2 weeks and since 18 weeks new bone with active osteon formation was poorly demarcated from compact bone. Calcium deposition of EG was early increased than that of CG deposition since 4 weeks after experiments. It was suggested that EG showed active and rapid new bone formation and similar bone mineralization of compact bone
5,400원
