간행물
대한구강악안면병리학회지 KCI 등재 The Korean Journal of Oral and Maxillofacial Pathology
- 발행기관 대한구강악안면병리학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1225-1577 (Print)2384-0900 (Online)
- 수록기간 1977 ~ 2024
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
제31권 제1호 (2007년 2월) 7건
1.
2007.02
구독 인증기관 무료, 개인회원 유료
Matrix metalloproLeases(MMPs) a re a family of zinc dependent enzymes with the capacity to degrade extracellular matrix prote ins. MMPs express ion correlates with cancer cell invasiveness and metas taslS The purpose of our sωdy was t。 determine the role 0 1' stroma l fïbrob lasts in ca ncer cell invasi on by examining the expression and activity of MMP-2 and -9 We used a YD- lOB squalllous ca rcinoma cell line that was est a blished in Yonsei Univer s ity ‘ College 0 1' Dentistry. We then appli ed two types of s lrolllal fibroblastsdel‘ ived from normal gingival tissue and cancer supporting ti ss ue Morphologic examination of fïbroblas ls was carried out by rnicroscopy and doubling time was measured by MTI assay . YD-lOB cells were cultured by lh ree-d imensional culture using collagen gel with two types of flbroblasts To examine the stromal - mesenchyma l inleraclion. we used a direct co-cul tu re system between YD-IOB cells and fibroblasts. Gelatin zymography was performed to exa llline MMP-2 a ncl 9 activit ies. We found that cancer-derived fibroblasts di splay stel late-shaped cells wi th many cyLopl asmic processes. whereas normal gingi val fibroblasts were spindle shaped. The c1oubling time of bOLh lïbroblasLs was not statistically different. Three-dimensional co-culture 0 1' ca ncer cells with ca n cer- c1erived fïbroblasts induced the formation 0 1' multi - Iayered atypical cells, as compared to culturing with normal gin gival f lbrobl asts Both three-c1 imens ional culture ti ss ues inclucecl the invasive gro￦th of cancer cells into the dermal eq uival enLs . Gelatin zymography s howecl that gelatinolytic activity of MMP-2 was activaterl in both co-culture models. However , MMP-9 ac li vity was not a lterecl in YD-lOB carcinoma cells In conclusion. enhanced MMP-2 activity was inc1 uced by boLh cance r- c1eri vecl a ncl norlllal gingival fibroblasts. suggesting that the potential to invacle by stromal fibroblasts was noL l imilecl to ca ncer- c1 eri ved lïbroblasts
4,000원
2.
2007.02
구독 인증기관 무료, 개인회원 유료
As a pa rt 0 1' the effort to develop a suitable scaffold for tissue-engineered bone regene ration, we modified calcium metaphosphate(CMP) ce ramic with 5 mol% Na20 or K20 to improve t he biodegradability and evaluated their effi ciency as a biodegr adable scaffold for ti ssue-engineered bone regeneration. The macroporous αiIP ceramic blocks incor porated with 5 mol% Na20 or K20 were prepa recl to have average pore size of 250 um in an inte rconnectecl framework structure The influ e nce of inco r pora tecl 5 mol% Na20 or K20 on cytotoxicity‘ cellular attachmont and t heir clifferentiation was evaluated by in vit ro analyzing sys tern. res pectively. The bioclegradability, histocompatibility, and osteogenic effect by cell-scaffolcl co nstruc ts were evalua ted by im plantation of them into subcutaneous pouches of SD-rats 0 1' SCID ITllce The incorporation of 5 mol% Na20 or K20 causecl clecrease of compressive strength without improving of biodegr adabili ty . The moclifi ed scaffolcls revea led no cy totoxic and excell ent biocompatability but osteogneic effect was recluced compa red to pure CMP ce ramic porous blocks . These res ul Ls s ugge::;t tha t the incorporation of 5 mol% Na20 0 1' K20 into pure CMP is not effective for improv ing effï ciency 0 1' scaffolcls fo1' tissue-engineered bone regeneration in terms of bioclegradabi li ty‘ physical s trength . a ncl osteogenic rege ne ra tive effect
4,000원
3.
2007.02
구독 인증기관 무료, 개인회원 유료
Effect of GW9662 on Proliferation and Osteoblastic Differentiation of Bone Marrow Derived Stem Cells
Previous ly we have s hown that fï brob last• growth factor-2 (FGF-2) and dexamethasone (Dex) in combination strongly stimulate both p l 이 i fe rati o n a nd differe nt iation of mesenchymal stem cells (MSCs) into osteoblasts and adipocytes, In the present s tudy we invesL igaLed whether inhibition 01' FGF-2 and Dex-induced adipogenic differentiation of bone marrow derived s Lem cells (BMSCs) by GW9662, an antagoni s t of proxisome proliferators-activated receptol γ (PPARy) which plays a key role in ad ipogenic differentiation , enhances proliferation and osteoblastic differentiation of BMSCs Proliferation 01' BMSCs t reated wi 네 FGF-2 a nd Dex was further increased by GW9662 up to 9,7, 10,6, and 7,2% at 3, 5, and 7 days of cul Lu re , Expansion of BMSCs with FGF-2, Dex and GW9662 followed by osteoblastic different iation showed that osteoblas tic differentiation 01' BMSCs was in creased by 37 % (p=O, 01) compared to those expanded with FGF-2 and Dex, ln contrast , ad i pogenic di fferenti a tion of FGF-2 and Dex-expanded BMSCs was substantially reduced to 14% (p=O, 036) by GW9662, Taken toget her , these resul ts demonstrate that FGF-2 and Dex in combination with GW9662 f ur t her stimu late proliferation 01' BMSCs and those cells expanded with these factors acquire enhanced potentiaIs to be dif ferentiated i n to osteoblas ts
4,000원
4.
2007.02
구독 인증기관 무료, 개인회원 유료
Methic illin - resistant StaphyJococcus aureus(MRSA) is not only a common cause of nosocomial infections worldwide but also leading to seri ous illnesses with high rates of mortali ty , This study was performed to determine resistance patterns and molecul ar biological ca racteri slics on MRSA isolated from clinical specimen, The purpose is to provide valua ble in formation related to t he purs uit 0 (' in('ec tion trace and infe<:tion control The test of susceptibility to 17 kinds antibiotics showed that these bacterias were susceptible to glycope ptides s uch as vancomycin, teicoplanin by 100%, rifarnpin by 65, 8%, They we re a lso 100% resistance to peni cillin , cefotaxim, gentamycin and aminoglycoside drugs , and showed 68, 56% multidrug res is ta nce to a bove 13 a ntibi oti cs incl uding peni cillin, 1n the analysis of MRSA using PCR, mecA was detected in every MRSA at 35 s t ra i ns a nd l'emA in 94 1%, ln a test , to de tect toxic genes, six genes, such as sea, seb(8, 6% res pectively)‘ sec(51, 4%) ‘ seg, se i(65 , 7% res pectively) and seh(2 , 9%) were found Finally , in resisrance chract eristics, toxic genes we re isola ted c1 ifferently acco rcling to specimen but there was no c1ifference res istance pattern ancl toxic genes The c1ata from t his s t ucly contribule to a more prec ise knowledge about the resistance pa ttern of MRSA in a clinical sett ing in k orea ancl regarcl exa mi na ti on of resis ta nce pattern accorcling to molecular biological methocl are expectecl to provide bas ic informa ti on on MRSA di ssemination and infection contro
4,000원
5.
2007.02
구독 인증기관 무료, 개인회원 유료
Human saliva conta ins a la rge number of proteins and peptides whose composition may alter as a conseq uence of disease. '1'0 date‘ however. the proteins and peptides that routinely populate t his oral fluid are largely unknown, '1'0 provid e a ca ta logue 이， sali va protei ns. we have surveyed the unstimulated human whole saliva by using shotgun proteomics. F'or the shotgun a pproach‘ whole sali va proteins were digested into peptides with ChemDigestD and the res ulting pe ptide fragments were sepa rated by RP- HPLC, followed by each fraction was tryptic di gestion ChemDiges tD- Trypsin diges ted pe pt ides were analyzed by tandem mass spectrometry (MS!MS) using a nano-LC equi pped quadru po le-time of fli ght rnass spect rometer, and the obtained spectra were searched against human protein sequence da tabase us ing MASCOT Shotgun proteomics a llowed a total of 291 human proteins to be confidently assigned. The largest gro u p (17 , 2%) of the identifi ed proteins sorted into functional categories was included in the s ignal transducti on function except for the hypothet ical or unknown functio n, This work provides a valuable starting point for the ana lys is of human sa l i va ry protei ns a nd theil‘ biological functions and candidates from human whole saliva that may prove to be of diagn ost ic and t herapeutic s ignif‘ Ica nce
4,300원
6.
2007.02
구독 인증기관 무료, 개인회원 유료
This is a case reporL of a ra re mi xed odontogenic tumo r, amelob lastic fibro-odontoma in the poste1'ior of r ight ma nd tlbe A 2-year - olcl ma le pa ti enL was referred to our department ['or large tumorous lesion 011 ri ght mandible Radiograph ic examination s howed la rge radi opaque and rad iolu cent lesion with impacted and unerupted tooth, #44‘ #45 , #46 #85 , AJ'Ler s urgi cal enuclea Li on & cu rettage of t he mass , the tumor was confirmed to ameloblastic fibro-odontoma. lt was composed with c1 enta l orga ns a ncl is la nd of odontogeni c epithelium embedded in a cell - ri ch mesenchymal s troma, AIso‘ we ca rri ed out an immunohi stochemical study. The resul ts s howed positive CK7 staining. and showed weekly positi ve for Bcl - 2‘ Ki - 67 s Laining, while CEA, CK8‘ CKl2, CKl6 showed l1egative, Follow-up studies have shown no tumot recurrence for 2 yea rs ,
4,000원
7.
2007.02
구독 인증기관 무료, 개인회원 유료
Cherubi sm is a ra re autosoma l dominant inherited condi tion caused by mutations in the c-Abl-binding protein SH3BP2. 1t is characteri zecl by mul t iple cystic giant cell lesions of the jaw appearing in early childhood with stabi li zation and rcmi ssion after pubcr ty, In thc present study, genomic DNA was purified f rom a blood sample obtained from the patient a ncl pa rents a ncl used f'or di rect sequence analysis of the SH3BP2 gene, 1n addit ion, a sample of the lesion was used f0 1" hi stologic ancl immunoh is toche mical purposes, Histology revealed a proliferation of spindle s haped fibroblas t ic cells and irregu la ry dis persed multinucleated giant cell s , The multinucleated giant cells proved posit ive for CD68 and TRAP, Ge nomic DNA sequencing f'ou ncl a missense mutation Pro418Arg in exon 9 of the SH3BP2 gene of the patient and the mother, Theref'ore, the mul t inucleated giant cells are basically osteoclastic in nature, Additionally, as the PI'o418Arg mutation had been repol' ted as caus ing cherubism, it represents a mutational hotspot,
4,000원
