본 연구는 마음챙김에 기초한 인지치료(Mindfulness Based Cognitive Therapy: MBCT) 프로그램이 수형자의 정신건강과 분노행동에 미치는 효과를 탐색하고자 수행되었다. 연구대상은 P 교도소 수형자 20명이었으며, MBCT 프로그램은 Segal 등(2002/2006)의 MBCT 프로그램을 수형자들의 정신건강 향상에 초점을 두어 수정보완한 것이었다. 회기당 120분(휴식 10분 포함), 8회기로 구성되었으며 주 4회씩 2주간 진행되었다. 프로그램의 효과검증을 위해 켄터키 마음챙김기술척도와 SCL-90-R(간이정신진단검사), 분노행동척도를 사용하였다. 그 결과 참여자들의 마음챙김수용이 사전에 비해 향상되었고, 간이정신진단 검사의 하위요인인 강박증, 불안, 적대감, 편집증, 정신증이 감소했으며, 분노행동의 분노직접표출이 감소하고 자기표현이 향상된 것으로 나타났다. 이 결과는 마음챙김에 기초한 인지치료가 수형자들의 정신건강의 향상과 분노행동 감소에 효과적으로 적용될 수 있음을 의미하고, 마음챙김훈련이 수형자들의 적응적인 수형생활과 사회적응 및 재범률 감소에 긍정적으로 활용될 수 있음을 시사한다.

The functional cardiovascular system is comprised of distinct mesoderm-derived lineages including endothelial cells, vascular smooth muscle cells and other mesenchymal cells. Recent studies in the human embryonic stem cell differentiation model have provided evidence indicating that these cell lineages are developed from the common progenitors such as hemangioblasts and cardiovascular progenitor cells. Also, the studies have suggested that these progenitors have a common primordial progenitor, which expresses KDR (human Flk-1, also known as VEGFR2, CD309). We demonstrate here that sustained activation of BMP4 (bone morphogenetic protein 4) in hESC line, CHA15 hESC results in KDR+ mesoderm specific differentiation. To determine whether the KDR+ population derived from hESCs enhances potential to differentiate along multipotential mesodermal lineages than undifferentiated hESCs, we analyzed the development of the mesodermal cell types in human embryonic stem cell differentiation cultures. In embryoid body (EB) differentiation culture conditions, we identified an increased expression of KDR+ population from BMP4-stimulated hESC-derived EBs. After induction with additional growth factors, the KDR+ population sorted from hESCs-derived EBs displays mesenchymal, endothelial and vascular smooth muscle potential in matrix-coated monolayer culture systems. The populations plated in monolayer cultures expressed increased levels of related markers and exhibit a stable/homologous phenotype in culture terms. In conclusion, we demonstrate that the KDR+ population is stably isolated from CHA15 hESC-derived EBs using BMP4 and growth factors, and sorted KDR+ population can be utilized to generate multipotential mesodermal progenitors in vitro, which can be further differentiated into cardiovascular specific cells.

Techniques to evaluate gene expression profiling, such as sufficiently sensitive cDNA microarrays or real-time quantitative PCR, are efficient methods for monitoring human pluripotent stem cell (hESC/iPSC) cultures. However, most of these high-throughput tests have a limited use due to high cost, extended turn-around time, and the involvement of highly specialized technical expertise. Hence, there is an urgency of rapid, cost-effective, robust, yet sensitive method development for routine screening of hESCs/hiPSCs. A critical requirement in hESC/hiPSC cultures is to maintain a uniform undifferentiated state and to determine their differentiation capacity by showing the expression of gene markers representing all three germ layers, including ectoderm, mesoderm, and endoderm. To quantify the modulation of gene expression in hESCs/hiPSC during their propagation, expansion, and differentiation via embryoid body (EB) formation, we developed a simple, rapid, inexpensive, and definitive multimarker, semiquantitative multiplex RT-PCR platform technology. Among the 9 gene primers tested, 5 were pluripotent markers comprising set 1, and 3 lineage-specific markers were combined as set 2, respectively. We found that these 2 sets were not only effective in determining the relative differentiation in hESCs/hiPSCs, but were easily reproducible. In this study, we used the hES/hiPS cell lines to standardize the technique. This multiplex RT-PCR assay is flexible and, by selecting appropriate reporter genes, can be designed for characterization of different hESC/hiPSC lines during routine maintenance and directed differentiation.

This is a case reporL of a ra re mi xed odontogenic tumo r, amelob lastic fibro-odontoma in the poste1'ior of r ight ma nd tlbe A 2-year - olcl ma le pa ti enL was referred to our department ['or large tumorous lesion 011 ri ght mandible Radiograph ic examination s howed la rge radi opaque and rad iolu cent lesion with impacted and unerupted tooth, #44‘ #45 , #46 #85 , AJ'Ler s urgi cal enuclea Li on & cu rettage of t he mass , the tumor was confirmed to ameloblastic fibro-odontoma. lt was composed with c1 enta l orga ns a ncl is la nd of odontogeni c epithelium embedded in a cell - ri ch mesenchymal s troma, AIso‘ we ca rri ed out an immunohi stochemical study. The resul ts s howed positive CK7 staining. and showed weekly positi ve for Bcl - 2‘ Ki - 67 s Laining, while CEA, CK8‘ CKl2, CKl6 showed l1egative, Follow-up studies have shown no tumot recurrence for 2 yea rs ,