산림청 희귀식물과 환경부 멸종위기식물로 지정된 섬현삼의 보전전략 수립을 위한 기초자료로 활용하고자 자생지 분포와 식생현황을 조사하였다. 현지조사를 통해 섬현삼은 북면 현포리, 천부리와 울릉읍 저동리를 중심으로 주로 해안가와 일주도로를 따라 분포하며 특히, 기존의 분포지역과 지역적으로 구분되는 서면에서 신규 자생지를 확인하였다. 각 지역별로 조사된 자생지는 해발 6∼96m의 범위에 분포하며 경사는 3∼40˚로 석축, 전석지, 바위틈에 분포하였다. 조사한 10개 자생지에서 총 2,002개체를 확인하였으며 북면 천부리에서 765개체(38.2%)로 가장 많았고 신규 자생지인 서면은 10개체(0.4%)로 가장 적은 개체가 분포하였다. 섬현삼의 전체적인 개체변화는 큰 변화의 폭이 없었으나 성숙개체(40.0%)보다 1∼2년생 미성숙개체(60.0%)의 비율이 높아 추후 환경변화 및 위협요인으로 인한 급격한 감소가 우려된다. 이에 섬현삼 현지내·외 보전전략으로 경쟁식물에 의한 도태 및 피압과 같은 위협요인의 제거, 정부기관 및 자자체의 행정적 관리, 국립수목원을 비롯한 연구기관의 적극적인 활동 등 보전방안을 수립하였으며 추후 섬현삼 현지외 보전·확대에 필요한 기초자료가 될 것으로 판단된다.

The objective of this study is to estimate the effect of adding TES to LEY and FGE freezing extender for the sperm viability, acrosomal morphology and DNA fragmentation from miniature pig sperm, we evaluated sperm characteristics in TFGE, TLE and LEY with various thawing condition ( for 20 sec, 45 sec and for 5 sec, respectively), and in different concentration of glycerol at 1%, 1.5%, 3%. The sperm viability and normal acrosome intact(NAI) in TFGE (Viability : , NAI : ), TLE (, ) extender significantly(p<0.05) increased than that in LEY (, ) extender thawed at for 5 sec. According to the results from glycerol concentration, the viability and NAI of miniature pig sperm in 1.5% glycerol TLE (, ) was highest among the experimental groups. In accordance with this, DNA fragmentation rates was the lowest in TLE () while that in LEY () is the highest. Therefore, these results suggest that TLE extender method for freezing- thawing of miniature pig sperm increased the viability after thawing.

Matrix metalloproteinases (MMP) play important roles in extracellular matrix (ECM) remodeling during ovarian follicular development, oocytes development and ovulation. In an attempt to investigate the effect of MMP activation in development cumulus-oocytes complexes, we examined the localization and expression of MMP, and monitored MMP expression profile. Cumulus-oocytes complexes were collected and matured in vitro for 24 hr, 36 hr and 48 hr. A mRNA expression of MMP-2, MMP-9, TIMP-2 and TIMP-3 was detected in all culture medium regardless of CC, OC and COCs. Activity of MMP-2 in the OC progressively was increased from 24 hr to 48 hr. But MMP-9 was not detected in all culture medium. The localization of MMP-2 was also measured by immunohistochemistry analysis. The MMP-2 and TIMP-2 was detected in cumulus cell and oocyte zone pellucida. Expression of MMP-2 protein in the COCs was progressively increased from 24 hr to 48 hr. However, MMP-9 protein was progressively decreased from 24 hr to 48 hr. And TIMP-2 protein was most highly expressed in the COCs 36 hr. Expression of TIMP-3 protein in the COCs was progressively increased from 24 hr to 48 hr. In conclusion, these results suggest that MMP-2 plays a role in maintaining normal maturation and development by controlling the ECM inhibitor concentration on cumulus cell and oocytes.

Artemisia scoparia (A. scoparia), perennial herb is indigenous to Korea and has been traditionally used in liver damage. We investigated the effect of the essential oil obtained from A. scoparia on apoptosis of KB cells. Cytotoxicity and cellular DNA content were analyzed by MTT assay and flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. We found that the essential oil induced the apoptosis of the KB cells by concentrations of 0.4 to 0.2 mg/ml which was verified by DNA fragmentation, apoptotic bodies, and the sub-G0/G1 ratio. The essential oil also transient caspase-9 and caspase-3 activity and cleavage of PARP in KB cells for 24 h. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. In conclusion, we demonstrated that the essential oil of A. scoparia induces apoptosis in KB cells

Relaxin과 insulin이 돼지 난포 과립막세포의 스테로이드 호르몬 분비에 미치는 영향을 연구하기위하여 체외에서 황체화된 과립막세포에서 prosesterone과 의 생산을 조사하였다. 돼지난포 과립막세포를 혈청 존재하에 배양접시에 부착 후 48시간 동안 체외배양하고 무혈청 배지에서 24시간 배양하였다. Relaxin과 insulin의 용량의존성을 확인하기 위하여 다양한 농도 (10, 100, 1,000 ng/ml)를 각각 무혈청 배지에 첨가하였다.

SUMMARY I. 서 론 II. 재료 및 방법 III. 결 과 IV. 고 찰 V. 적 요 VI. 인용문헌