본 연구에서는 식품공전에 제시되어 있는 microwave digestion 전처리방법과 유도결합 플라즈마 방출분광기을 이용하여 식품 중의 9종의 무기질(Na, Ca, K, P, Mg, Fe, Cu, Mn 및 Zn)을 측정하는 분석법에 대한 직선성, 정밀성 및 정확성 등의 분석법 유효성 검증을 실시하였다. 본 연구에 사용된 표준시료는 Certificated reference material1849a 조제분유로 미국 national institute of standards & technology에서 구입하였다. 직선성은 표준품을 사용한 표준검정곡선 측정농도범위에서 상관계수 0.9999 이상의 양호한 결과를 나타내었다. 검출한계는 0.1005 mg/kg, 정량 한계는 0.3351 mg/kg 으로 각각 나타났다. 또한 정밀도는 상대적표준편차(relative standard deviation)가 일내(withinday, n=3), 반복측정의 경우 0.09~4.80%, 일간(between-day, n=12)의 경우 1.19~18.19%로 양호한 결과를 나타내었으며 정확성은 회수율 90.35-110.63%로 매우 양호한 결과를 나타내었다. 따라서 microwave 전처리방법과 유도결합 플라즈마 방출분광기 측정법은 식품 중 9종의 무기질을 측정하는데 매우 유용할 것으로 사료되며 국민 건강 증진을 위한 식품성분표 데이터베이스 구축, 유통식품의 품질평가 등 공익적 분석사업에 이용될 수 있을 것으로 생각된다.

Recurrence-metastasis status of squamous cell carcinoma of tongue is a challenging oncologic problem. This study examined the expression of E-cadherin/β-catenin cell adhesion complex in squamous cell carcinoma of the tongue through an immunohistochemical study. Twenty samples from 15 patients with squamous cell carcinoma of the tongue, who were treated at the Department of Oral and Maxillofacial Surgery, consisted of primary or recurrent tumors along with matched metastatic lymph nodes were retrieved for immunohistochemical staining and grouped based on recurrence-metastasis status.Differences in stain localization were noted in E-cadherin, β–catenin and phospho β–catenin staining between different tumor groups based on the recurrence-metastasis status. The number of phospho β-catenin stain positive cells was found to have a significant role in survival. E-cadherin confirms its role as a powerful individual differentiation indicator and the role of β-catenin specially the phospho type elicts interest

A blood test is a laboratory analysis performed on a blood sample that is usually extracted from a vein in the arm using a needle, or via fingerprick. They are used to determine physiological and biochemical states, such as disease, mineral contents, drug effectiveness, and organ function. Although the term blood test is used, most routine tests (except for most haematology) are done on plasma or serum, instead of blood cells. Main advantage of using saliva in diagnostics is easy and non invasive sample taking compared to peripheral blood. According to the study published, saliva contains more than 20 percent of the proteins found in blood. The purpose of present study is to compare biochemical enzymes in saliva and in blood serum and to evaluate the usefulness of saliva specimens instead of blood in dental clinic. The saliva from 215 healthy over 50 years of aged people lived in Dong-gu district, Gwangu city was collected and the analysis was performed by six enzyme-linked immunosorbent assays (ELISA). ELISA results were compared with blood chemistry results. The values or patterns on Alanine Aminotransperase (ALT), Aspartate Aminotransperase (AST), Cholesterol and Triglyceride in saliva were not correlated with those in blood serum. However, Albumine and γ-glutamyl transpeptidase (γ-GTP) were followed the positive relationship with blood chemistry. These result showed that detection and identification of Albumine and γ-GTP level could be established by saliva ELISA analysis, so that ELISA assay on saliva could be useful alternative to serum testing.