The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

The development of Information Communication Technologies (ICTs) has dramatically changed the way of hotel booking. The increasing number of online consumers provides abundant data for demand forcasting in revenue management. The current methods e.g. historical data analysis, normally focus on studying consumers’ behaviors and preferences in the hotel but may not be able to integrate that out of the hotel such as dining, travelling, shopping and entertaining, which will bring crucial references to the co-relationship between these consumption and the way of hotel booking. This research adopts a persona approach to infer consumers’ preference probability by using a variety of real-time data. Quantitative methods are employed. In order to predict the booking needs accurately, this research establish a Bayesian network on the online platform for life service that can be associated with various consumer behavior data. The results indicate that in the environment of uncertain demand, the algorithm is effective and applicable, which will help directors of hotel revenue management in accurate price planning and decision making.

In Korea, a serious amphibian disease caused by the fungus Batrachochytrium dendrobatidis (Bd) has been reported from historical samples collected in the 1900s. In this study, we continue to evaluate the current prevalence of chytridiomycosis in the Korean Peninsula and we include imported frogs from America to our analysis. Non-invasive skin swabs were taken from 275 apparently healthy frogs, and Bd was detected in five free living frogs by the nested PCR protocol consisting of two species: Bombina orientalis and Rana catesbeiana, from Gyeongnam and Cheonbuk provinces. These frogs comprised about 2% of the total number of free living samples. This study might be useful for understanding amphibian chytridiomycosis in Korea.

This paper proposes combination of a cognitive agent architecture named Soar (State, operator, and result) and ROS (Robot Operating System), which can be a basic framework for a robot agent to interact and cope with its environment more intelligently and appropriately. The proposed Soar-ROS human-robot interaction (HRI) agent understands a set of human’s commands by voice recognition and chooses to properly react to the command according to the symbol detected by image recognition, implemented on a humanoid robot. The robotic agent is allowed to refuse to follow an inappropriate command like “go” after it has seen the symbol ‘X’ which represents that an abnormal or immoral situation has occurred. This simple but meaningful HRI task is successfully experimented on the proposed Soar-ROS platform with a small humanoid robot, which implies that extending the present hybrid platform to artificial moral agent is possible.