Ramie (Boehmeria nivea L. Gaudich.) is a hardy perennial herbaceous plant of the Urticaceae family and has been grown as a fiber crop in several countries including Korea for many centuries. Ramie leaves also have been traditionally used as a major ingredient of a type of rice cake called ‘Song-pyun’ in the Southwest area of Korea, especially Yeong-Gwang province. Despite its economic importance, the molecular genetics of ramie have not been studied in detail yet. Genetic resources of ramie were widely collected from domestic local sites by Bioenergy Crop Research Center (RDA) and Yeong-Gwang Agricultural Technology Center. For the systematic and efficient management of the genetic resources, we developed microsatellite molecular markers of ramie. To do this, we generated microsatellite-enriched genomic DNA libraries using magnetic bead hybridization selection method. 216 contigs containing microsatellite repeat motif were generated using nucleotide sequences of 376 clones from the libraries. Primer sets were designed from the flanking sequences of the repeat motif. Finally, we selected 26 microsatellite markers, possibly showing polymorphism among the genetic resources. Results on the genotype analysis of the ramie genetic resources using the microsatellite markers will be presented.

Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival in such an environment. The key component of innate immune system, C3 is central protein of all activation pathways of the complement system, leading to inflammatory reactions, such as opsonisation, chemotaxis, and cell lysis of pathogens. Although, innate mechanisms is essential for survival in the early stage of development, little is known about defence mechanisms. In this study, the alignment analysis showed that amino acid sequence of C3 from olive flounder liver EST homologous to other known C3 sequences with 73-99% identity. Also, we examined the tissue distribution of olive flounder C3 and analyzed expression pattern from the fertilized egg until 28 days post hatching. As a result, olive flounder C3 mRNA was expressed only in the liver and the mRNA level more increased as developmental proceed during the early stage. These results may suggest that olive flounder C3 plays an important function in the early immune response of olive flounder larvae.

Development of transgenic plant with desirable traits to cultivated plant is one of the important procedures in plant molecular breeding. However, applicable assessment of transgenic plant in laboratorial scale is not much except cultivating transgenic plant for a whole life in field condition. Here, we analyzed chlorophyll fluorescence in three transgenic soybean lines with AtMYB44 transcription factor for assessment of photosynthetic activity under abiotic stresses such as drought. Soybean varieties used in this study were ‘Bert’ and ‘Bert’ derived three transgenic soybeans, ‘AtMYB44 CM35101’, ‘AtMYB44 CM2471’, and ‘AtMYB44 CM4481’. Analyzed five different chlorophyll fluorescence variables are maximum PSII quantum yield (QY_max), steady state PSII quantum yield (QY_Lss), steady state non-photochemical quenching (NPQ_Lss), coefficient of photochemical quenching in steady-state (Qp_Lss), and fluorescence declineratio in steady-state (Rfd_Lss). To determine main chlorophyll fluorescence variable affected by abiotic stress, principal component analysis (PCA) was conducted with five chlorophyll fluorescence variables measured from four varieties. QY_Lss and NPQ_Lss were main chlorophyll fluorescence variables to evaluate abiotic stress, particularly in drought stress. In comparison with transgenic soybean lines based on chlorophyll fluorescence variables, ‘AtMYB44 CM2471’ and ‘AtMYB44 CM4481’ are more tolerant to drought than the others. Interestingly, three transgenic soybean lines which have a same AtMYB44 gene with different regions of chromosome revealed the quite different responses of chlorophyll fluorescence to drought treatment.

Shinnamjag is a round, smooth, white-skinned, and fleshed potato cultivar with erect plant shape and early maturity. It has excellent table quality. Its yields are high, especially with prominent marketable yield compared to Superior, a leading potato cul

The optimized in vitro culture system was investigated for improvement of regeneration efficiencies by observing the responses of scutella-derived callus of Korean rice (Oryza sativa L.). Large variations of callus induction (43.9-93.9%) and shoot regeneration (0-88.7%) were observed among the rice cultivars depending on medium. However, shoot regeneration was significantly improved by selected utilization of basal medium, growth regulators, and carbon sources. N6 basal medium was more efficient for embryogenic callus induction than MS or LS basal medium, while MS was superior to N6 for shoot regeneration. The calli of highly regenerative cultivars grew faster and showed higher rates of green tissue formation (GT) and shoot regeneration (SR) and lower rate of callus browning (CB) than those of recalcitrant cultivars. Although a higher level of kinetin stimulated the GT and SR in highly regenerative cultivars, 10~textrmmgL-1 kinetin generally suppressed the GT and SR, while CB was accelerated compared to 2~textrmmgL-1 kinetin. Additional benefits of sorbitol combined with maltose (or sucrose) under 5~textrmmgL-1 kinetin were certainly confirmed on regeneration efficiencies compared to sucrose alone as carbon source and osmotic regulator. This combination showed high rate of GT and SR with multiple shoots while low rate of CB. With MSRK5SM-Pr medium (5~textrmmgL-1 kinetin, 3% sorbitol, 2% maltose, 500~textrmmgL-1 proline), the regeneration efficiencies of total 17 out of 24 cultivars were practically improved 160% on average compared to MSRK2S (2~textrmmgL-1 kinetin, 3% sucrose) control medium. Especially, the medium was most effective to the cultivars showing a medium level of regenerability such as Daesanbyeo and Dongjinbyeo and Suwon477, enhancing efficiencies more than 300-600% compared to MSRK2S medium.

Most of recommended potato varieties in Korea are white skin colored. Purple skinned potatoes have been recognized as being effective in the prevention of gastric disease. A new variety “Jaseo” has dark purple skin and white flesh colors, and attractive r

‘Jowon’ is a new early-maturing potato variety for table use. It has spreading growth habit with narrow oval leaves. Its tubers are attractively round with white skin and light yellow flesh color. The yielding ability of Jowon is similar to Superior. Jowo

다량의 대기오염물(SO2, HF)이 배출되고 있는 공업단지 주변에서 재배되고 있는 수도의 생육에 이들 대기오염물이 미치는 영향을 구명하기 위하여 수도의 각종 형질, 수량, 대기중의 오염물 농도, 엽내유황 및 불소함량과 엽피해율을 조사하여 시험한 결과는 다음과 같다. 1. 엽내유황함량과 대기중 아황산가스 농도간에는 높은 정의 상관이 인정되었다. 2. 엽피해율 조사치는 대기오염에 의한 수량감소의 가장 중요한 지표로 이용 할 수 있다. 3. 본 조사지역에서는 아황산가스보다 불화수소의 배출량이 적지만 엽피해의 발생에는 불소의 관련성이 아황산가스보다 높았다. 4 수량형질의 수량에 대한 기여정도는 주당수수가 제일 컸으며, 주당수수는 엽내 불소함량과 깊은 관련성이 인정되었다. 5. 엽내 유황함량은 수량 및 수량형질과 상관성이 인정되지 않았다.