Photodynamic therapy(PDT) 는 특정 광 감응 물질 에 광 조사를 수행하여 세 포내애서 활성 산소종(ROS) 의 증가를 통한 암세 포의 사띨을 유도하는 방법이다 광 감응 물질은 암세 포에 선택 적으로 흡수되어 특정 파장의 빛을 홉수하여 다량의 ROS를 발생하여 암세 포의 사멸을 유도한다， 그러나 PDT 수행 시 ROS의 세 포내 작용에 따른 사띨 기전 이 영확히 알려지 지 않았고， 비 선 택 성으로 인한 정 상 세포애서의 피해 도 유발될 수 있다고 보고되었다 본 연 구애서 는 굉 감웅제인 He ma to por p h y rin을 이 용하여 구깅암 세포주인 He p2에서 광조사를 통한 세포 사멸 에 관한 연 구를 수행하였다 Confocal mi crosco py를 통한 분석에서 Hemato po r phyrin은 홉수 시 간에 따라 세포막에서 세 포질 핵 으로의 위치함을 관찰 할 수 있었고. 전기 영 동에 따른 DNA 분절 분석에서 24 시간이상 홉수된 상태 에서 ladder 패턴을 보임으로써 세 포 자띨사에 이 르는 만웅을 보였다 DCF - DA에 의힌 세 포내 ROS 분석 을 수행힌 결과 Hema to po rphyrin의 흡수 시간이 증가할수록 세포 내부에서의 ROS 발생이 증가함을 획 인 할 수 있었 다 이와 같은 결과에 따르면 Hematoporphyrin을 이용한 PDT에서 h ematoporphy ri n의 홉수 시 간에 따라 세 포 자띨사가 유도되었고. 특히 Hematoporphyrin은 흡수 시 간이 증가하여 세 포 내 부까지 충분히 Hematoporphyri n 이 작용된 경우에 서 ‘ ROS 발생애 따른 미 토콘드리아 의존적 경 로를 통해 세 포 자멸사가 유도되 었음을 확인 할 수 있었다-

Department of Oral Pathology, Dental Science Research Institute, College of Den디'suy， Chonnam National University It is well known that the expansion of radicular and dentigerous cyst is related to the change of osmotic pressure, while the prolifera디on ofthe 비피19 epithelium in odontogenic keratocyst. When the dentigerous cyst and odontogenic keratocyst has secondary infection, they present the loss of unique sπuctures and epithelial hyperplasia. There is a question whether inflammatory reaction affects cystic formation, expansion and their treatment. Present study is to evaluate the relationship between inflammation and epithelial hyperplasia using immunohistochemial study. Followings are result; 1. 까1e age of patients ranged from 10 to 73 years (mean age, 39 years) in radicular 이5t， 10 to 71 years (mean age, 35 years) in dentigerous cyst and 10 to 54 years (mean age, 23.4 years) in odontogenic keratocyst. The sex distribution of patient distributed 32 cases for male and 16 cases for female in radicular cyst, 13 and 10 in dentigerous cyst and equally 5 and 5 in odontogenic keratocyst. 2. Inflammatory cyst in the present study distributed 44/48 cases (9 1.7%) for radicular cyst, 15/ 23 cases for dentigerous cyst and 1/ 10 case for odontogenic keratocyst. 3. Evaluation of inflammatory reaction and antigen expression, ki-67 , cox-2 and glut-1 expression increase in the inflammatory radicular and dentigerous cyst. 4. In odontoge띠c keratocyst, expression of antigens increase regardless of inflammation. Above results showed that inflammation stimulate the proliferation of lining epithelium leading to cystic expansion.

Eugenol (4-allyl-2-methoxyphenol) is a phenol derivative and generally used in dental treatment. A few investigator reported that eugen이-induced C)πoto잉city by apopto디c pathway, but it is not yet well understood In the present study, to investigate the eugenol-induced cytoto잉city by apoptosis, we have examined the apoptotic molecules and pathway in primary human gingival fibroblast (HGF) and human salivary gland cells (HSG). To identify apoptotic cell death, 3-(4,5-dimethylthiazol-2-yl)-2 ,5-diphenyl tetrazolium bromide (MTT) reduction assay with or without N-acetylcysteine (NAC), and the morphological study by propidium iodide (pI) staining were screened. And to investigate the apoptotic pathway, reverse transcriptase-polymerase chain reaction (RT-PCR) for apoptotic molecules and caspase aαivity assay were performed. Both M1T reduction assay and an addition of NAC showed that eugenol act as a pro-oxidant led to cell death. With the morphological study, both cells showed apoptotic change by nuclear fragmentation and/or chromatin condensations. With the apoptotic machinery study, the Bax and Bcl-2 mRNA expression were not detected in HGF. But, for HSG, the increased expression of Bax with decreased of Bcl-2 was observed. And the expression of Apaf-l was not detected or nα significantly increased in HGF and HSG, respectively. With measure of caspase activity, there was no change of caspase activities in HGF. But, for HSG, there was decrease of caspase 9 activity and increased caspase 3 activity. We suggest eugenol-treated HGF underwent apoptosis independent of Bcl family and caspase. However, for eugenol-πeated HSG, apoptosis occurred via Bcl famiIy and caspase pathway.