In this study, rye, Hairy beaches, pastures long and weighing many organic grazing cover crops can repress the uniforms, and the tractor was designed and built for mounting repression device. And identify appropriate working conditions and job factors and job performance by analyzing the correlation between the test results are as follows. Level 40∼50HP tractor for applying cover crops repression device width 2,400㎜, battling roller diameter is 380㎜, battling back after work uniforms repress blade for 4×100㎜, 640㎏ gross weight was optimized. Optimization of field test the tractor speed shift 1(0.45㎧∼0.53㎧) and in the repression and crop height 40∼ 60㎜ uniforms, respectively. Appropriate working conditions identified by weight in the roller by type by speed test results, the weight is heavy, long repression and squeezed me, speed was increased, the lower the efficiency of repression.

In ruminants, Interferon-τ (IFN-τ) has the role of recognizing pregnancy signals produced by the embryo and it may have an important role during the luteolysis. Therefore, the purpose of the present study was to investigate the effect of IFN-τ on prostaglandin synthesis, cyclooxygenase-2 (COX-2) gene expression in vitro and secretion of progesterone (P4) in vivo. The epithelial and stromal cells isolated from bovine endometrium were cultured with different doses of IFN-τ (0, 0.02, 0.2 and 2 μg/ml). Human chorionic gonadotropin (hCG, 1.5 IU/ml) was used as a positive control. Prostaglandin E2 and F2α levels in the culture media were analyzed by enzyme immunoassays, and total RNA was extracted from the cells for RT-PCR. P4 concentrations in blood samples were assayed by chemiluminescent immunoassay system. In epithelial cells, COX-2 gene expression was increased in the presence of IFN-τ (p<0.05), but it was not significantly different in all groups of stromal cells except 2 μg/ml IFN-τ group (p<0.05). Although IFN-τ did not affect PGE2 and PGF2α production in epithelial cells, it decreased PGE2 and PGF2α production significantly in stromal cells (p<0.05). In vivo experiment, the P4 concentrations in blood sample was significantly increased after injection of 1 μg/ml IFN-τ. These results indicate that PG production was mediated by COX-2 expression in the stromal cells but it did not affect in the epithelial cells, and suggest that treatment of IFN-τ was to improve the implantation environment of uterine by maintenance of high P4 concentration. * This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ907008)” Rural Development Administration, Republic of Korea.