We studied the infection rate of and various metacercariocidal approaches to controlling Gymnophalloides seoi for prevention of human infection in cultured and natural oysters in Korea. The selected survey areas were Aphae-do (Shinan-gun, Jeollanam-do), which is an endemic area for G. seoi, and Tongyeong (Geonsangnam-do), which is the main production area of oysters in Korea. In the Tongyeong area, the metacercariae of G. seoi were not detected in cultured oysters (0/201) or wild oysters (0/134). Seventy-two G. seoi metacercariae were observed in 33 of 265 natural oysters collected from Aphae-do; however, metacercariae were not detected in the cultured oysters (0/1101) purchased from the Daejeon Fish Market. To investigate the viability of G. seoi metacercariae, various metacercariocidal treatments were used with 3.5% saline and oyster juice used as positive controls. The metacercariae survived for 75.4 h in 3.5% saline and 112.6 h in oyster juice. After the metacercariocidal treatment, G. seoi metacercariae were survived for 13.29 min in tap water, < 20 sec in 4.3% vinegar, no effect in a rinse of the whole oyster body in 70°C water for 1 sec, but 1 sec in a rinse of the whole oyster body in 90°C water for 1 sec. The greatest metacercariocidal effect on G. seoi was from rinsing oysters in 90°C water followed by those from treatment with 20% ethyl alcohol, 4.3% vinegar, and tap water. However, we suggest that the most actual prevention to G. seoi human infection is rinsing the oysters with tap water for at least 30 min.

This study used SCS-CN method to estimate the real recharge of the study area which is one of the most reasonable techniques to estimate groundwater recharge when there is no available runoff data in a watershed. From the results of the real recharge analysis for the study area using SCS-CN method, it was analyzed that the year 1994 when the drought was severe showed the lowest recharge of 106.3mm with recharge rate of 12.4%, and the highest recharge of 285.6mm with recharge rate of 21.8% occurred in 1990. Yearly average recharge of 213.2mm was obtained, and the average recharge rate was 16.9%/year. KOG-FLOW model which has powerful post process functions consists of setting environments for input parameters in Korean language, and help function is added to each input data. Detailed information for each parameter is displayed when the icon is placed on the input parameters, and geologic boundaries or initial head data for each layer can be set easily on work sheet. The relative errors (R. E.) for each model’s observed values and calculated values are 0.156~0.432 in case of KOG-FLOW, and 0.451~1.175 in case of WINFLOW, therefore it is known that KOG-FLOW model developed in this study produced results compared to observed head values.