This study aimed to improve and refine climate exposure indicators developed in a previous study by incorporating and analyzing two years of Italian ryegrass field trial data from the Jinju and Jangheung regions. The objective was to evaluate the relationships between productivity and climatic factors. The field trial results demonstrated that plant height, fresh yield, and dry matter yield of Italian ryegrass was significantly higher in 2018–2019 than in 2017–2018. Precipitation and temperature during the winter and spring seasons of 2018–2019 were also greater than those recorded in 2017–2018. Correlation analyses revealed significant positive associations between productivity and precipitation in Oct, Dec, Feb, and Mar. Productivity was also positively correlated with mean temperature and mean minimum temperature in Oct, Dec, Jan, Feb, Mar, and Apr, as well as with growing degree days in Oct, Dec, Jan, Feb, and Mar. The climate exposure indicators derived in this study are expected to serve as valuable tools for predicting Italian ryegrass productivity and assessing vulnerability to climate impacts. Nevertheless, considering the complex interactions between climatic factors and field conditions, further refinement through additional experiments and analyses remains necessary.

Rye, whole-crop barley and Italian Ryegrass are major winter forage species in Korea, and yield monitoring of winter forage species is important to improve forage productivity by precision management of forage. Forage monitoring using Unmanned Aerial Vehicle (UAV) has offered cost effective and real-time applications for site-specific data collection. To monitor forage crop by multispectral camera with UAV, we tested four types of vegetation index (Normalized Difference Vegetation Index; NDVI, Green Normalized Difference Vegetation Index; GNDVI, Normalized Green Red Difference Index; NGRDI and Normalized Difference Red Edge Index; NDREI). Field measurements were conducted on paddy field at Naju City, Jeollanam-do, Korea between February to April 2019. Aerial photos were obtained by an UAV system and NDVI, GNDVI, NGRDI and NDREI were calculated from aerial photos. About rye, whole-crop barley and Italian Ryegrass, regression analysis showed that the correlation coefficients between dry matter and NDVI were 0.91∼0.92, GNDVI were 0.92∼0.94, NGRDI were 0.71∼0.85 and NDREI were 0.84∼0.91. Therefore, GNDVI were the best effective vegetation index to predict dry matter of rye, wholecrop barley and Italian Ryegrass by UAV system.

This study is conducted to screen compounds affecting ruminal fermentation under in vitro incubation. Saponin, chitosan, metformin, zinc acetate, zinc chloride, zinc oxide, zinc sulfate, ascorbic acid, oil-coated ascorbic acid, nicotinic acid, eastern herb cocktail, charcoal extract and garlic sources (lyophilized and extract) were added to rumen culture fluid at 1.25% of substrate (100% timothy) volume at 24 h incubation. pH, total gas, ammonia, VFAs were measured. Ascorbic acid increased total gas production indicating fermentation level. All zinc compounds significantly decreased (p<0.05) although zinc sulfate increased propionate of volatile fatty acid (p<0.05). In following experiment, ascorbic acid, oil-coated ascorbic acid, garlic lyophilized, herb cocktail and zinc sulfate were added to rumen culture fluid at 2.5% of substrate with 3, 6, 12 and 24 h incubation. Zinc sulfate decreased both ruminal fermentation and VFAs production but ascorbic acid enhanced total gas production. Ascorbic acid increased fermentation regardless of supplement concentration although excessive zinc sulfate decreased fermentation. These results suggest that optimal level of trace compounds might affect ruminal fermentation in ruminant.

본 연구는 홀스타인, 한국재래산양 및 한우유로부터 glycomacropeptide(GMP)를 분리하였으며, 각 GMP의 trypsin 가수분해물의 혈소판응집 억제 효과를 in vitro 상에서 알아보았다. 홀스타인, 한국재래산양 및 한우의 GMP는 분자량이 모두 약 20 KDa이었으며, sialic acid 함량은 각각 36.86±2.36, 37.98±1.27 및 31.19±1.87㎍/mg이었다. 또한 모든 개체의 GMP에서 tyrosine이 검출되었다. 홀스타인, 한국재래산양 및 한우 GMP의 trypsin 가수분해물에 의한 혈소판 응집 억제율은 반응 30초에 4.02, 5.51 및 12.77%로 각각 나타나 시간이 경과할수록 감소하는 경향을 보였다. 혈소판의 현미경 관찰 결과 가수분해물 첨가 후 혈소판 수가 증가하였으나, 첨가 후 30초가 경과한 시점부터 혈소판 수가 감소하기 시작하여 120초 후에는 관찰 할 수 없었다. 본 실험 결과 bovine 및 caprine GMP의 trypsin 가수분해물에서 혈소판 응집을 억제할 수 있는 small peptide가 있는 것으로 생각되며, 향후 이러한 연구는 심근경색증 및 뇌혈전증을 예방할 수 있는 생리활성 물질로 이용될 수 있을 것이라 생각된다.