Background: The Nordic hamstring exercise is a closed-chain exercise that effectively activates all lower extremity muscles, but there is a lack of consideration of ankle joint which affects exercise effectiveness. One of the major advantages of the Nordic exercise have been known as strengthening lower extremity extensors. This manifests the exercise increases activities of the ankle plantar flexors as well as hip extensors. Objectives: To investigated the impacts of changes in ankle position on the lower extremity musculature and subjective difficulties during the Nordic hip extension exercise. Design: Cross-sectional study. Methods: Twenty the healthy male participants participated in this study. Participants perform three variations of the Nordic exercises including three ankle positions. Each Nordic exercise was defined as ankle position (neutral, dorsiflexion and plantar flexion). During the Nordic exercise, erector spine (ES), gluteus maximus (GM), biceps femoris (BF), gastrocnemius (GCM) activities and subjective difficulties (Borg RPE score) were measured. Results: Muscular activities of the biceps femoris and gastrocnemius were significantly different among the three ankle conditions, which showed controversial results (P<.05). The biceps femoris activity was significantly higher with the dorsiflexion condition, compared to the others (P<.05). On the other hand, activity of the gastrocnemius was significantly higher with the plantarflexion position compared to the others (P<.05). Conclusion: Present study suggests the ankle positional changes could affect lower muscular activities during the Nordic exercise. Although it is difficult to affirm the causes due to limited data, selective activation of the biceps femoris might accomplish with ankle dorsi flexion and that of the gastrocnemius might accomplish with the ankle plantarflexion during the Nordic exercises.

본 연구에서는 flavonoid apigenin과 baicalein의 세포 실험으로 콜라겐 회복효과를 확인하고 전사체 비교 분석을 통하여 collagen 회복 효과를 뒷받침할 수 있는 결과를 제안하고자 한다. 연구 결과 apigenin과 baicalein은 HS68 세포주에서 UV에 의해 손상된 I형 collagen 회복에 효과를 보였으며, 두 물질 모두에서 ECM 관련 유전자들의 발현 변화를 확인하였다. ECM을 조절하는 공통적인 메커니즘을 보일 뿐 아니라 각각 다른 범주의 유전자들을 변화시키는 결과도 보여서, 세포에 다양한 영향을 미치는 것으로 예측할 수 있었다.

Background: Although several studies have reported functional improvements after forward walking training on a treadmill and after backward walking training on a treadmill, there is a lack of immediate effects data, investigating spatiotemporal parameter, neuromuscular recruitment. Objectives: To compare the immediate effects between forward and backward walking on treadmill training, present study measured muscle activities of the lower extremity, gait parameters, and dynamic balance. Design: Cross-sectional study. Methods: The twenty-four asymptomatic young male subjects were participated in this study. Subjects have performed 15-min of forward and backward walking on treadmill. Before and after treadmill walking, the gait parameters were measured with the GAITRite. The dynamic balance abilities were assessed with the Y-balance test. Muscular activities were collected from the tibialis anterior, gastrocnemius, biceps femoris, and rectus femoris during forward and backward walking. Results: Muscular activities of the biceps femoris and tibialis anterior were significantly different between forward and backward walking conditions, which were higher with the backward walking (P<.05). Compared to the pre-measurement, the normalized dynamic balance composite score of the post-measurement was significantly higher with the backward walking (P<.05). Regardless of the treadmill training method, there were no significant differences between pre and post measurement, both the stride length and step length (P>.05). Conclusion: The performing backward walking training has positive effects for dynamic balance.

Canine parvovirus (CPV) remains a leading infectious cause of death in canines, especially in young puppies. Though vaccination is being carried out regularly, immunization failures occur, and puppies may be exposed to infection. Virus-like particles (VLPs) act like a subunit vaccine, mimicking the structure of authentic viruses. Therefore, VLPs have the potential to be used as vaccine candidates. Since Viral Protein 2 (VP2), a major structural protein of CPV, is the crucial antigen for CPV, the purpose of this study was to produce a recombinant VP2 of new canine parvovirus-2a using the baculovirus expression system in SF9 insect cells. The results revealed that recombinant VP2 assembles to form VLPs with antigenic properties similar to those of natural CPV, the recombinant VLP can produce a hemagglutination assay (HA) titer (1:210) in SF9 cells. Expression of the recombinant 6-His-tagged VP2 in SF9 cells was confirmed by western blotting. These findings suggest that the recombinant VP2 expressed in this study could be used as an efficient subunit vaccine against CPV infection.

Porcine parvovirus 2 (PPV2) was recently detected in the Republic of Korea. This paper reports two near-complete genome sequences of PPV2 identified for the first time in the lung tissue of aborted pig fetuses.

Viral protein 2 (VP2), which is the structural protein of parvovirus, can produce virus-like particles (VLPs) by a self-assembly process in vitro, making VLPs attractive vaccine candidates. VP2 of canine parvovirus (CPV) is responsible for neutralizing antibodies in immunized animals. In this study, VP2 protein of canine parvovirus-2c was expressed using a baculovirus expression system and assembled into parvovirus-like particles in insect cells. The results show that VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and a high hemagglutination (HA) titer (1:27). The recombinant 6-His-tagged VP2 protein with a molecular mass of about 65 kDa was detected by anti-His antibody and anti-PPV serum. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of CPV and in the vaccination against CPV.

Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

본 연구는 흰점박이꽃무지의 안정적인 생산과 저장법 확립을 위해 수행되었다. 이를 위해 일정 기간과 온도 조건에서 흰점박이꽃무지 유충의 저장 특성을 조사하였다. 저장 시험에 사용된 유충의 평균무게는 2.1g인 개체를 사용하여 다양한 온도조건(5℃, 10℃, 15℃, 20℃, 및 25℃)에서 일정한 용기에 각각 400마리씩 넣은 후 15주간 저장처리하였다. 조사결과 각 처리구별로 초기 저장 무게에 비해 각각 47.4%, 59.4%, 65.8%, 62.6%, 및 92.8%로 변화하였으며, 생존율은 82.8%, 94.3%, 94.8%, 91.3%, 및 94.6% 이었다. 노숙된 3령 유충(개체평균 무게 2.5g)과 성장 중인 유충(개체평균무게1.8g)을 각각 500개체씩 넣은 후 10℃에서 5개월 저장 후 체중변화와 생존율을 측정한 결과 체중은 각각 86.3%, 75.2%, 생존율은 94.6%, 96.2%이었다. 저온저장시 성장 중인 유충에 비해 노숙된 유충의 체중감소율이 적게 나타났다.