Tube, an intracellular protein of the Toll-pathway, forms a complex with Pelle and MyD88, and regulates a signal transduction to activate NF-κB in Drosophila. To understand the antimicrobial function of TmTube, the induction patterns of TmTube were investigated at 3, 6, 9, 12, and 24 h-post injection of pathogens into 10th to 12th instar larvae. In addition, we investigated the effects of TmTube RNAi on larval mortality and tissue specific AMP expression in response to microbial challenge. Our results will provide a basic information to elucidate the immunological function of TmTube

The baculovirus expression system (BES) utilize the p10 or polyhedrin promoter, a very late promoter that exhibits strong transcriptional activity primarily at the end of viral infection, to produce useful recombinant proteins. The burst sequence of the very late promoter is essential for strong transcription, and VLF-1 is a transcription factor that binds specifically to the burst sequence, and it has been reported that it can regulate the amount and timing of expression of protein by the very late promoter. Recently, a VLF-1 constitutively expressing cell line was constructed to increase the production of the target protein, but the effect was minimal. In this study, to find the optimal VLF-1 expression conditions to increase target protein production efficiency, we controlled the expression of VLF-1 through various promoters and evaluated the target protein expression efficiency by the p10 promoter accordingly.

Hand, foot, and mouth disease (HFMD) is a highly contagious disease with no specific treatment. Since it is common in immunocompromised children under the age of 5, there is a need to develop a safe vaccine. Virus-like particles (VLPs) are similar structures to viruses with the lack of genetic material which makes them impossible to replicate and infect, and therefore have a high level of biological safety and are considered to have high value as vaccines. In this study, the insect virus expression system that is widely used for vaccine and drug production due to its high post-translational modification efficiency, was used to produce VLPs for Coxsackievirus type A6 and A10, which are recently reported to be the main causes of HFMD. For this purpose, the selection of promoters that can control the timing and intensity of expression of 3CD protein, which is essential for VLPs assembly but has been reported to be cytotoxic, was conducted to construct an optimal expression form for HFMD-VLP.

In Korea, most temporary storage facilities for spent nuclear fuel are nearing saturation. As an alternative to this, the 2nd basic plan for high-level radioactive waste management specified the operation plan of dry interim storage facility. Meanwhile, the NSSC No. 2021-19 stipulates that it is necessary to evaluate the possibility and potential effect of accident before operating interim storage facility. Therefore, this study analyzed the categories of accident scenarios that may occur in dry storage facility as part of prior research on this. We investigated the case of categorization of dry storage facility accident scenarios of IAEA, NRC, KAREI, and KINS. The IAEA presented accident scenarios that could occur in on-site dry storage facility operated with silo and cask method. NRC has classified accident scenarios in dry storage facility and estimated the probability of accidents for each. KAERI and KINS selected major accident scenarios and analyzed the processes for each, in preparation for the introduction of dry storage facility in Korea in the future. Overall, a total of 10 accident scenarios were considered, and the scenarios considered by each institution were different. Among 10 scenarios, cask drop and aircraft collision were included in the categorization of most institutions. The results of this study can be used as basic data for cataloging accidents subject to safety evaluation when introducing dry interim storage facility in Korea in the future.

아메리카동애등에 성충은 음식물 폐자원 등 유기물이 있는 곳에 알을 낳는 습성이 있다. 대부분의 농가는 음식 물폐자원을 가공한 단미사료(습식사료)를 유인배지로 활용하여 그 위에 플로랄폼(오아시스)를 놓고 알을 받는 다. 그러나 플로랄폼은 재사용이 불가하고 생분해되지 않는 환경폐기물로서 처리가 곤란하며 포름알데하이드, 카본 블랙 등의 발암물질을 함유한 것으로도 알려져 있다. 이에 본 연구는 먹이원 자체를 활용하여 폐기물이 발생하지 않는 친환경 산란받이를 개발하였으며 일회용으로 사용되는 플로랄폼을 대체하였다. 먹이원으로 활 용할 수 있는 습식사료와 건식사료를 주재료로 하여 제작하며, 습식사료(수분60~80%)와 건식사료(1~10%)를 1:0.5~1 비율로 혼합한 사료 혼합물과 보조첨가제와 물을 포함하여 제작한다. 친환경 산란받이는 기존 플로랄폼 대비 산란율이 34% 증가하였으며 구매비용 또한 75% 절감하였다.

표면발현(surface-display system)은 세포 또는 바이러스 표면에 목적 단백질을 고정하여 발현시킴으로써 목적 단백질에 대하여 독립적인 공간 구조 및 생물학적 활성을 부여하는 단백질 공학 기술이다. 또한 이를 이용하여 높은 중화항체 유도 및 대량생산이 가능한 삼량체의 형태로 항원 단백질의 발현 또한 가능하다. BES(baculovirus expression system)에서의 표면발현 기술은 번역 후 수정과정 및 복잡한 구조의 다양한 단백질의 발현이 가능하기 에 다른 숙주 기반 시스템보다 효율적이라고 보고되고 있다. 그러나 목적 단백질 외의 다른 표면 단백질과 발현 공간에서의 경쟁으로 목적 단백질의 낮은 생산량이 큰 문제점으로 지적되고 있다. 따라서, 이러한 BES에서 표면 발현의 생산 효율을 증대시키기 위하여, 동일한 표면 공간에 대한 단백질 간의 발현 경쟁에 대해 실험적으로 확인 후, 그를 해결하기 위하여 표면발현에 최적인 목적 단백질 발현을 위한 프로모터 선발 실험을 수행하였다. 이를 통해 BES에서 표면발현에 의한 목적 단백질의 생산 효율을 증대시킬 수 있음을 확인하였다.

In Korea, the construction of dry storage facilities for spent nuclear fuel is being promoted through the 2nd basic plan for high-level radioactive waste management. When operating dry storage facilities, exposure dose assessment for workers should be performed, and for this, exposure scenarios based on work procedures should be derived prior. However, the dry storage method has not yet been sufficiently established in Korea, so the work procedure has not been established. Therefore, research is needed to apply it domestically based on the analysis of spent nuclear fuel management methods in major overseas leading countries. In this study, the procedure for receiving and storing spent nuclear fuel in a concrete overpack-based storage facility was analyzed. Among the various spent nuclear fuel management systems, the metal overpack-based HI-STAR 100 system and the concrete overpackbased HI-STORM 100 system are quite common methods in the United States. Therefore, in this study, work procedures were analyzed based on each final safety analysis report. First, the HI-STAR 100 overpack enters the facility and is placed in the transfer area. Remove the impact limiter of the overpack and install the alignment device on the top of the overpack. Place the HI-TRAC, an on-site transfer device, on top of the alignment unit and remove the lids of the two devices to insert the canister into the HI-TRAC. When the canister transfer is complete, reseat the lid to seal it, and disconnect the HI-TRAC from the HI-STAR 100. Raise the canister-loaded HI-TRAC over the alignment device on the top of the HI-STORM 100 overpack and remove the lids of the two devices that are in contact. Insert the canister into the HI-STORM 100 and reseat the lid. The HI-STORM 100 loaded with spent nuclear fuel is transferred to the designated storage area. In this study, the procedure for receiving and storing spent nuclear fuel in a concrete overpack-based storage facility was analyzed. The main procedure was the transfer of canisters between overpacks, and it was confirmed that HI-TRAC was used in the work procedure. The results of this study can be used as basic data for evaluating the exposure dose of operating workers for the construction of dry storage facilities in Korea.