During an investigation of indigenous prokaryotic species in the Republic of Korea, 17 bacterial strains belonging to the phylum Bacteroidota and 4 strains belonging to the phylum Bacillota were isolated from freshwater environments. These isolated bacterial strains formed a robust phylogenetic clade with type strains of the closest related bacterial species, with 16S rRNA gene sequences sharing similarities of higher than 98.7%. To date, there have been no official report of these 21 isolates in the Republic of Korea. At the genus level, these unreported species were affiliated with Mucilaginibacter and Pedobacter of class Sphingobacteriia, Flavobacterium and Gillisia of class Flavobacteriia, Hymenobacter of class Cytophagia, and Paenibacillus and Planococcus of class Bacilli. These species were further examined by performing Gram staining, analyzing their colonies and cell morphologies, and determining their basic biochemical characteristics and phylogenetic positions. Detailed descriptions of the 21 previously unreported species are provided.

목적: 본 설문조사는 2010년 대한민국 안경원에서 판매된 콘택트렌즈의 종류에 대한 추세를 조사하고자 하였다. 방법: 1,000개의 설문지를 2010년 8월부터 9월 사이에 지역별 안배를 하여 전국이 안경원에 우편으로 발송하였다. 각 설문지는 각 안경원에서 설문지를 받은 후 판매된 처음 10건의 콘택트렌즈에 대한 정보를 적어 반송하도록 하였다. 결과: 설문조사가 진행된 2달 동안, 9.1%의 설문지가 회수되었으며, 총 872건의 콘택트렌즈 처방에 대한 정보를 수집하였다. 콘택트렌즈를 처방받은 고객의 평균 나이는 26.2±7.0세였으며, 76%가 여성고객으로 조사되었다. 소프트렌즈 처방이 91%였으며, 하드렌즈 처방은 9%로 나타났다. 소프트렌즈 처방중에서 일회용착용렌즈가 차지하는 비율은 60%였으며, 하이드로겔 매일착용은 17%, 그리고 15%가 실리콘하이드로겔로 판매/처방이 되었으며, 연속착용렌즈는 8%로 나타났다. 디자인별로는 75%의 소프트 렌즈가 일반 구면렌즈였으며, 토릭렌즈는 10%로 조사되었다. 미용렌즈는 15%를 차지하였다. 교체주기로써는 대부분의 소프트렌즈가 매일교체(35%) 혹은 3~6개월주기 교체(55%)로 조사되었다. 결론: 본 설문조사는 토릭렌즈의 처방은 다소 적었으며, 대신 미용렌즈의 판매/처방이 높은것으로 나타났다. 또한 멀티포컬 콘택트렌즈의 처방의 거의 없는것으로 조사되었다.

Dual-color fluorescence in situ hybridization karyotype analysis was created using repetitive sequences including two types of rDNA repeats (45S and 5S rDNAs) and Arabidopsis-type telomere sequence repeats. The somatic metaphase cells of Carthamus tinctorius were observed as diploids (2n=2x=24). A symmetrical or slightly asymmetrical karyotype with seven pairs of metacentric and five pairs of submetacentric chromosomes was observed. The lengths of the somatic metaphase chromosomes ranged from 4.18 to 6.53 ㎛, with a total length of 60.71 ㎛. One locus of 45S rDNA was located on the pericentromeric regions of three pairs of chromosomes and the other pair was situated on the terminal regions of the short arms of a single pair of chromosomes. One locus of 5S rDNA was detected on the interstitial regions of the short arms of two pairs of chromosomes. Arabidopsis-type telomeric repeats were detected on the terminal regions of all pairs of chromosomes. Co-localization of loci between telomeric repeats and 45S rDNA was observed in a single pair of chromosomes. The results provide additional information for the existing physical mapping project of C. tinctorius and will also serve as a benchmark to a more intricate cytogenetic investigation of C. tinctorius and its related species.