본 연구에서 Nematus tibialis를 국내에서 처음으로 보고한다. 이 종의 사진과 진단, 색 변이, 발육 단계와 분포 등을 제공하였다.

Background: Forward head posture (FHP) is a postural alignment of the cervical vertebrae that leads to increased gravitational load on cervical segmental motions. The overhead arm lift test assesses the ability to actively dissociate and control low cervical flexion and move the shoulders through overhead flexion. Objects: The purpose of this study was to explore muscle activities in the upper trapezius (UT), serratus anterior (SA), sternocleidomastoid (SCM), and lower trapezius (LT) alongside changes in head position during the overhead arm lift test in individuals with FHP. Methods: Fifteen subjects with forward head posture and fifteen subjects with normal subjcects were enrolled in this study. The patients performed the overhead arm lift test, and muscle activities of the UT, SCM, SA, and LT were measured using surface electromyography and by evaluating changes in head position. Independent t-tests were used to detect significant differences between the two groups and Cohen’s d was calculated to measure the size of the mean difference between the groups. Results: The FHP group demonstrated significantly increased muscle activity of the UT (32.46±7.64), SCM (12.79±4.01), and LT (45.65±10.52) and significantly decreased activity in the SA (26.65±6.15) than the normal group. The change in head position was significantly higher in the FHP group (6.66±2.08) than the normal group. Effect sizes for all parameters assessed were large between the two groups. Conclusion: The subjects with excessive FHP displayed were unable to fix their heads in position during the overhead arm lift test. The overhead arm lift test can thus be used in clinical settings to confirm control of the neck in these subjects.

본 연구를 통해 Spathius속의 Spathius depressithorax Belokobylskij, 1998 <납작가슴자루배고치벌 (신칭)>를 우리나라에서는 처음으로 보 고한다. 본 종의 식별형질과 성충을 검경하고 도해하였으며, 한국산 Spathius속의 목록을 제시한다.

본 연구를 통해 Centistes Haliday (벌목: 고치벌과: 잎벌레고치벌아과)에 속하는 7종 C. (Ancylocentrus) ater (Nees), C. (A) mucri Belokobylskij, C. (A.) paupella (Shenefelt), C. (A.) rufus Chen and Achterberg, C. (Centistes) pumilio Belokobylskij, C. (C.) tsherskii Belokobylskij, C. (Syrrhizus) delusorius (Förster)을 한국에 처음으로 보고한다. 또한 각 종에 대한 식별형질과 숙주 정보, 사진 등의 정보를 포함하 였다.

본 연구를 통해 2종의 Asiacentistes Belokobylskij(아시아넓은칼고치벌속: 신칭)(벌목: 고치벌과)를 보고한다. 그 중 Asiacentistes sinica Chen and Belokobylskij(긴칼집아시아넓은칼고치벌: 신칭)는 한국 미기록종으로 보고한다. 식별형질과 동아시아내 분포 정보를 포함하였다

본 연구를 통해 2종의 큰턱고치벌속(벌목, 고치벌과)을 보고한다. 수원큰턱나무좀고치벌 Cosmophorus (Cosmophorus) klugii Ratzeburg 1종 은 한국미기록종으로 보고한다. 식별형질과 분포 등의 정보를 포함하였다.

본 연구를 통해 한국산 Syntretus속의 Exosyntretus아속(벌목, 고치벌과)에 속하는 2종을 보고한다. 그 중 Syntretus (Exosyntretus) nevelskoii Belokobylskij은 국내 미기록종으로 보고한다. 전세계 기록종 목록, 식별형질과 분포 등의 정보를 포함하였다.

The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est2R) was 2,120 bp in length, encoding a protein of 516 amino acid residues with a calculated molecular weight of 57,286 Da. The molecular weight of the enzyme was estimated to be 57,000 Da by SDS-PAGE. Est2R shared 35.6% amino acid identity with esterase (CAH19079) of uncultured prokaryote. The Est2R was most active at 20-40°C, and showed optimum at 30°C and pH 8.0. The most activity of Est2R for the different chain length of p-nitrophenyl ester group as substrate was p-nitrophenyl acetate. Moreover, the enzyme was found to be most active without organic solvent, followed by 98% active with ethanol, and the enzyme activity was highly affected by the acetonitrile. The enzyme was significantly inhibited by Zn2+ but stimulated by Ca2+. So, novel esterase gene est2R is likely to obtain from cow rumen metagenome and supposed to use for industrial purpose.

A carboxymethyl cellulase gene, cel5B, was cloned, sequenced, and expressed in Escherichia coli. pRCS20 in E. coli was identified from metagenomic cosmid library of cow rumen for cellulase activity on a carboxymethyl cellulose agar plates. Cosmid clone (RCS20) was partially digested with Sau3AI, ligated into BamHI site of pBluescript II SK+ vector, and transformed into E. coli DH5α. The insert DNA of 1.3 kb was obtained, designated cel5B, which has the activity of hydrolyzation of CMC. The cel5B gene had an open reading frame (ORF) of 1,059 bp encoding 352 amino acids with a signal peptide of 48 amino acids and the conserved region, VIYEIYNEPL, belongs to the glycosyl hydrolase family 5. The molecular mass of Cel5B protein expressed from E. coli DH5α exhibited to be about 34 kDa by CMC-SDS-PAGE. The optimal pH was 8.0, and the optimal temperature was about 50℃ for its enzymatic activity.

This study was conducted to investigate the physiological activities of the water and methanol extracts from Auricularia auricula-judae(black and brown) and Auricularia polytricha. Largely, anti-oxidant, anti-tumorigenic, anti-inflammatory, anti-diabetic activities were carried out. DPPH scavenging activity was performed on the anti-oxidant test. MTT assay and nitrite scavenging acitivity were performed on the anti-tumorigenic test. The cancer cell were used to HeLa, A549, HT-29 and MCF-7 and normal cell was used as control. lastly, in the anti-inflammatory and anti-diabetic activities, NO assay and α-amyloglucosidase inhibition were performed, repectively.