,  , Pseudanostirus ecarinatus (Stepanov, 1930) is recognized for the first time in Korea. A redescription and illustrations of the species are presented based on a single male specimen collected from Mt. Bangtae Gangwon-do. We also provide diagnostic characteristics of the genus, which clearly separate it from other closely related genera, Calambus Thomson, 1859 and Anostrius Thomson, 1859.

We reviewed the Korean species belonging to the Tribe Dendometrini. Of the previously recorded five species in Korea, Athousius humeralis (Miwa, 1927) and Limoniscus rufipennis (Lewis, 1894) are confirmed as the misidentification of Ampedus basalis (Mennerheim, 1852) and Corymbitodes sp., respectively. It is suggested to exclude these species in the Korean fauna. In addition, the records of Limonius eximius (Lewis, 1874) and Limoniscus vittatus (Candèze, 1873) are also questionable since no specimens available in this study. We actually examined three species throughout this study. Limoniscus kraatzi ((Candèze, 1879) is recognized as the mostly abundant species in the Korean fauna. A new species, Cidnopus nigronitidus sp. nov. and a new record of Limoius scutellaris (Dolin, 2003) comb. nov. are recognized in Korea.

DNA barcoding is a strong species identification tool for all animal taxa, and can easily be conducted when materials are under DNA friendly conditions. In contract, a full-length (659 bp) sequencing has been limited for the degraded DNAs extracted from old museum specimens. The initial challenges to retrieve the authentic DNA fragments from old museum specimens were attempted by obtaining short sequences (<300 bp) with the cloning process after PCR, making it both expensive and time-consuming. In this study, we employed a modified method to analyze the full-length DNA barcoding regions in 31~52 year-old butterfly specimens (301 dried specimens of 39 species) using direct sequencing after PCR with two different methods: 1) the successful PCR rates of 0 to 5.6% using four universal primer sets were too low to obtain authentic sequences and the cross-contamination was detected in almost all successful amplicons; 2) the success rates of PCR using specie-specific overlapping primer sets were distinctly high, reaching up to 75% with 98% authentic and 2% non-specific sequences. Thus, the result showed the method that using species-specific primer set per species yields the most effective success rates of both PCR and sequencing from degraded DNA without incorrect sequences.

In DNA barcoding, the DNA degradation of old museum specimens has been limited full-length (658bp) sequencing. The challenges associated with the retrieval and authentication of degraded DNA extracts from fossil and old museum specimens were principally limited to analyze the relatively short sequences (<300 bp). Furthermore, almost protocols in other to analyzed the degraded DNA contained the cloning process after PCR causing the time-consuming and the rising costs. To overcome these problematic circumstances, we tried a modified method to analyze full-length of DNA barcoding region in 30~60 year-old butterfly specimens (225 samples in 28 species), using direct sequencing after PCR with species-specific overlapping primer sets per each species. As a result, all of 28 species have been successfully analyzed, although 178 samples (79%) are completely generated barcoding sequences ranged from 640 to 658 bp and 47 samples (21%) are partially sequenced ranged from 100 to 500 bp. Thus, the result showed that the direct PCR sequencing using the overlapping primer sets per species appears to have great potential efficiency for analysis of degraded DNA without incorrect sequences.

A new genus, belonging to the tribe Hemicrepidini of subfamily Dendrometrinae, from Taiwan is established. The new genus is compared to their related eight genera occurring in Taiwan by cladistic analysis based on diagnostic characters used in generic level.

Histeridae is a family of beetles commonly known as clown beetles and hister beetles and approximately 3,900 species in 11 subfamily have been described to date. In Korea, up to days, 53 species of 22 genera of 6 subfamily of them have been recorded. They can be easily identified by their shortened elytra that leaves two of the seven tergites exposed, and their elbowed antennae with clubbed ends. Most members of hister beetles are predators and found in most kinds of decaying organic matter, such as droppings, decomposing bodies of animals, compost piles and other decaying plant materials. Some species are also used to control livestock pests that infest dung or to control house flies (Ôhara, 1994). Recently, we have collected several histerid species with Lindgren funner trap for the ecological research on the bark beetles. As a result of this study, we added three newly recorded species, Niponius osorioceps Lewis, 1885, Plegaderus (Plegaderus) marseuli Reitter, 1877 and Trypeticus fagi (Lewis, 1884) to Korean fauna. In this presentation, diagnosis and photographs of characters for each species are presented.

We reviewed the Korean species belonging to the Tribe Dendrometrini. Of the previous Korean records. Athousius humeralis (Miwa, 1927) and Limoniscus rufipennis (Lewis, 1984) are confirmed as the misidentification of Ampedus basalis (Mennerheim, 1852) and Corymbitodes sp. recorded by Kishii and Paik (2002), respectively, and, in addition, the records of Limonius eximus (Lewis, 1874) and Limoniscus vittatus (Candeze, 1873) are also questionable since no specimens available in this study. A new species Cidnopus sp. nov. and a new record of Cidnopus koltzei (Reitter, 1895) is recognized in Korea. Limonius scutellaris (Dolin, 2003) comb. nov. is newly proposed. Tribe Dendrometrini Gistel, 1856 Athousisu humeralis (Miwa, 1927) - misidentification Cidnopus sp. nov. Cidnopus koltzei (Reitter, 1895) - new to Korea Limonius eximus (Lewis, 1894) - Korean specimen unavailable Limonius scutellaris (Dolin, 2003) comb. nov. - new to Korea Limoniscus kraatzi kraatzi (Candèze, 1879) Limoniscus vittatus ((Candèze, 1873) - Korean specimen unavailable Limoniscus rufipennis (Lewis, 1894) -misidentification

The Wolbachia bacterium, one of the most prevalent endosymbiotic bacteria, is known to induce reproductive anomalies such as cytoplasmic incompatibility, feminization, male killing and parthenogenesis in various arthropod species. The bacterium is considered to have had huge impacts on hosts' reproductive biology, immunity, evolution, and molecular machineries. Infection surveys on the bacterium have rather been limited to specific taxa that are mainly of economical importance or conducted with randomly collected organisms. Here we investigated infection frequency of Wolbachia in 206 Coleopteran insects collected from Korea. Among them 28 species (13.59%) across families proved to harbor Wolbachia. The phylogenetic trees based on the partial 16s rRNA gene and the partial Wolbachia surface protein (wsp) gene of Wolbachia show that all the Wolbachia strains belong to either Supergroup A or B and Wolbachia evolved independently from its hosts. In addition, the cophylogenetic analysis of the 16s rRNA gene and wsp gene implies that there have been horizontal DNA transfers and recombination events within and between divergent Wolbachia supergroups.