DNA methylation at CpG sites, which is a epigenetic modification, is associated with gene expression without change of DNA sequences. During early mouse embryogenesis, dynamic changes of DNA methylation occur. In this study, DNA methylation patterns of porcine embryos produced in vivo and in vitro were examined at various developmental stages by the immunocytochemical staining method. Interestingly, active demethylation was not observed on the paternal pronucleus of porcine zygotes. However, differences were detected in the passive demethylation process between in vivo and in vitro embryos. There was no change in the DNA methylation state until the blastocyst stage of in vivo embryos, whereas partial demethylation was observed in several blastomeres from a 4 cell stage to a morula stage of in vitro embryos. The whole genome of inner cell mass (ICM) and trophectoderm (TE) cells in porcine blastocysts were evenly methylated without de novo methylation. Our findings demonstrate that genome-wide demethylation does not occur in pig embryos during preimplantation development unlike murine and bovine embryos. It indicates that the machinery regulating epigenetic reprogramming may be different between species.

실내에서 담배거세미나방의 영기별 약제감수성을 조사한 결과 시험된 약제 모두 유충의 영기가 높아질수록 약제감수성은 낮았다. Chlorpyrifors-methyl과 chlorpyrifos의 독성은 3령부터, etofenprox+PAP와 deltamethrin은 2령부터 현저히 떨어졌다. 포트시험결과 deltamethrin을 제외한 4약제 모두 90% 이상의 방제가를 보여 담배거세미나방의 방제에 유용하게 사용될 수 있을 것으로 생각된다. 하지만 야외에서 담배거세미나방은 전 영기가 혼재되어 있고 영기별 약제감수성이 다르기 때문에 적절한 약제선택은 물론 유충 발생초기에 약제를 살포하는 것이 중요하다.

The Stockholm Convention was adopted in Sweden in 2001 to protect human health and the environment, including Persistent Organic Pollutants Rotors, such as toxic and bioaccumulative. Currently, there are 28 kinds of materials. This prohibits and limits the production, use, and manufacture of the product. Korea is a party to the Convention and it is necessary to prepare management and treatment plan to cope with POPs trends. In the text, we have discussed HCBD materials. HCBD belongs to halogenated aliphatic unsaturated hydrocarbons. It is a toxic, organic mixture of bioaccumulation. A study on the treatment of waste containing HCBD substance, We decided to treat the waste containing HCBD thermally. So six samples were selected. Waste water treatment sludge, rubber plate, insecticide, tarpaulin, tire rubber, mixed sample. The tire rubber injected HCBD as a technical sample. HCBD analysis showed that 59.345 ~ 18,238.355 ug/kg was detected. For the thermal treatment, we analyzed element. As a result of thermogravimetric analysis, the weight change due to the decomposition of the material started at 200℃. The material decomposition was completed within 800℃. The thermal treatment was performed on a Lab-scale (1kg/hr). After exhaust gas analysis result, HCBD was detected at 0.01 to 0.09 ug/kg. The decomposition rate is estimated to be 99.848 ~ 99.999%. As a result of dioxin analysis in the exhaust gas, the highest concentration was found in the tarpaulins and the emission limit was exceeded. The concentrations of Cd, Pb, Cr, Cu, Ni and Zn in the residues were very low. Considering the decomposition rate of HCBD containing wastes, incineration treatment at 2 ton/hr or more is considered to be possible. And unintentional persistent organic pollutants such as dioxins in the exhaust gas. Therefore, it is considered safe to operate the incineration temperature at more than 1100℃.

To achieve energy efficiency improvement is used to lower temperature for emission gas at catalyst inlet, or to reduce/stop using steam to reheat emission gas. Saved energy from this process can be used as power source in order to increase generation efficiency. Dry emission gas treatment, on the other hand, is the technology to increase generation efficiency by using highly efficient desalination materials including highly-responsive slaked lime and sodium type chemicals in order to comply with air pollution standards and reduce used steam volume for reheating emission gas. If dry emission gas is available, reheating is possible only with the temperature of 45℃ in order to expect generation efficiency by reducing steam volume for reheating. Retention energy of emission gas from combustion is calculated by emission gas multiplied by specific heat and temperature. In order to obtain more heat recovery from combustion emission gas, it is necessary to reduce not only exothermic loss from boiler facilities but emission calorie of emission gas coming out of boiler facilities. In order to reduce emission calorie of emission gas, it is efficient to realize temperature lowering for the emission gas temperature from the exit of heat recovery facility and reduce emission gas volume. When applying low temperature catalysts, the energy saving features from 0.03% to 2.52% (average 1.28%). When increasing the excess air ratio to 2.0, generation efficiency decreases by 0.41%. When the inlet temperature of the catalyst bed was changed from 210℃ to 180℃, greenhouse gas reduction results were 47.4, 94.8, 118.5, 142.2 thousand tons-CO2/y, CH4 was calculated to be 550.0, 1100.1, 1375.1, 1650.1 kg-CH4/y, and N2O was 275.0, 550.0, 687.6, 825.1 kg-N2O/y. In the case of high efficiency dry flue gas treatment, reduction of greenhouse gases by the change of temperature 120~160℃ and exhaust gas 5,000 ~ 6,500 ㎥/ton is possible with a minimum of 355,461 ton/y of CO2 and minimum 4,125 tons of CH4/y to a maximum of 6,325 ton/y and N2O to a minimum of 2,045 kg/y to a maximum of 3,135 kg/y.

"Early Valley", is an early maturing potato cultivar with high yield potential. "Early Valley" is a clonal selection resulting from the cross between 'Suncrisp' and 'A87109-10'. It has medium plant height and light green foliage. "Early Valley" has medium flowering habit and white flowers. Tubers are smooth, yellow skin, light yellow flesh, round tuber shape, medium eye depth, and medium dormancy and good keeping quality. It has stable yield under wide range of climatic conditions. "Early Valley" is resistance to late blight, but moderately susceptible to common scab and hollow heart. This cultivar is also resistant to potato rotting at harvesting during the raining season. "Early Valley" has high level of antioxidant activity (about three times higher) and vitamin C (higher by 40%) than the 'Superior'. This cultivar has high level of tuber uniformity and capable of yielding 36.56 t/ha which is 17.07% higher than the control potato cultivar 'Superior' under optimum agronomical practices.