실내에서 담배거세미나방의 영기별 약제감수성을 조사한 결과 시험된 약제 모두 유충의 영기가 높아질수록 약제감수성은 낮았다. Chlorpyrifors-methyl과 chlorpyrifos의 독성은 3령부터, etofenprox+PAP와 deltamethrin은 2령부터 현저히 떨어졌다. 포트시험결과 deltamethrin을 제외한 4약제 모두 90% 이상의 방제가를 보여 담배거세미나방의 방제에 유용하게 사용될 수 있을 것으로 생각된다. 하지만 야외에서 담배거세미나방은 전 영기가 혼재되어 있고 영기별 약제감수성이 다르기 때문에 적절한 약제선택은 물론 유충 발생초기에 약제를 살포하는 것이 중요하다.

우리나라산 주머니나방과의 1미기록종, 유리주머니나방(Acanthopsyche nigraplaga Wileman)을 보고하며 수컷 생식기 도해와 함께 외부형태를 간약하게 기술하였다. 도한 천일홍, 명석딸리 차조기, 새모래덩굴, 흰명아주 5종의 기주식물도 처음으로 확인하였다.

The Stockholm Convention was adopted in Sweden in 2001 to protect human health and the environment, including Persistent Organic Pollutants Rotors, such as toxic and bioaccumulative. Currently, there are 28 kinds of materials. This prohibits and limits the production, use, and manufacture of the product. Korea is a party to the Convention and it is necessary to prepare management and treatment plan to cope with POPs trends. In the text, we have discussed HCBD materials. HCBD belongs to halogenated aliphatic unsaturated hydrocarbons. It is a toxic, organic mixture of bioaccumulation. A study on the treatment of waste containing HCBD substance, We decided to treat the waste containing HCBD thermally. So six samples were selected. Waste water treatment sludge, rubber plate, insecticide, tarpaulin, tire rubber, mixed sample. The tire rubber injected HCBD as a technical sample. HCBD analysis showed that 59.345 ~ 18,238.355 ug/kg was detected. For the thermal treatment, we analyzed element. As a result of thermogravimetric analysis, the weight change due to the decomposition of the material started at 200℃. The material decomposition was completed within 800℃. The thermal treatment was performed on a Lab-scale (1kg/hr). After exhaust gas analysis result, HCBD was detected at 0.01 to 0.09 ug/kg. The decomposition rate is estimated to be 99.848 ~ 99.999%. As a result of dioxin analysis in the exhaust gas, the highest concentration was found in the tarpaulins and the emission limit was exceeded. The concentrations of Cd, Pb, Cr, Cu, Ni and Zn in the residues were very low. Considering the decomposition rate of HCBD containing wastes, incineration treatment at 2 ton/hr or more is considered to be possible. And unintentional persistent organic pollutants such as dioxins in the exhaust gas. Therefore, it is considered safe to operate the incineration temperature at more than 1100℃.

To achieve energy efficiency improvement is used to lower temperature for emission gas at catalyst inlet, or to reduce/stop using steam to reheat emission gas. Saved energy from this process can be used as power source in order to increase generation efficiency. Dry emission gas treatment, on the other hand, is the technology to increase generation efficiency by using highly efficient desalination materials including highly-responsive slaked lime and sodium type chemicals in order to comply with air pollution standards and reduce used steam volume for reheating emission gas. If dry emission gas is available, reheating is possible only with the temperature of 45℃ in order to expect generation efficiency by reducing steam volume for reheating. Retention energy of emission gas from combustion is calculated by emission gas multiplied by specific heat and temperature. In order to obtain more heat recovery from combustion emission gas, it is necessary to reduce not only exothermic loss from boiler facilities but emission calorie of emission gas coming out of boiler facilities. In order to reduce emission calorie of emission gas, it is efficient to realize temperature lowering for the emission gas temperature from the exit of heat recovery facility and reduce emission gas volume. When applying low temperature catalysts, the energy saving features from 0.03% to 2.52% (average 1.28%). When increasing the excess air ratio to 2.0, generation efficiency decreases by 0.41%. When the inlet temperature of the catalyst bed was changed from 210℃ to 180℃, greenhouse gas reduction results were 47.4, 94.8, 118.5, 142.2 thousand tons-CO2/y, CH4 was calculated to be 550.0, 1100.1, 1375.1, 1650.1 kg-CH4/y, and N2O was 275.0, 550.0, 687.6, 825.1 kg-N2O/y. In the case of high efficiency dry flue gas treatment, reduction of greenhouse gases by the change of temperature 120~160℃ and exhaust gas 5,000 ~ 6,500 ㎥/ton is possible with a minimum of 355,461 ton/y of CO2 and minimum 4,125 tons of CH4/y to a maximum of 6,325 ton/y and N2O to a minimum of 2,045 kg/y to a maximum of 3,135 kg/y.

Soluble-NSF attachment protein receptor (SNARE) proteins play a role in vesicle fusion, exocytosis, and intracellular trafficking in neuronal cells as well as in fertilization and embryogenesis. We investigated the expression patterns of two SNARE proteins, SNAP-25 and synaptotagmin VII (SytVII), and their regulation by pregnant mare serum gonadotropin (PMSG) during mouse ovarian follicular development. Ovaries were obtained at 0, 12, 24, 36, and 48 h post-PMSG injection of immature mice. SNAP-25 and SytVII mRNA expression levels increased gradually in a time-dependant manner. However, protein levels revealed different patterns of expression, suggesting different translational regulation following PMSG stimulation. SNAP-25 and SytVII expression was closely associated with thickening of the granulosa cell (GC) layer and follicle morphological changes from a flattened to a cuboidal shape. To explore follicle stimulating hormone receptor (FSHR)-mediated regulation of their expression, GCs from preantral follicles were cultured to examine the effects of FSHR siRNA knockdown. FSHR siRNA abolished upregulation of the SNAREs in both PMSG and FSH-stimulated GCs. This abolished gene expression was rescued by adding dibutyryl cyclic AMP to the cultures. These results suggest that SNAP-25 and SytVII expression is regulated via the FSHR-cAMP pathway during follicular development.

Construction of the Asian Network for Sustainable Organic Farming Technology (ANSOFT) will be cooperatively administered by the public researchers in 12 Asian member countries from 2010 to 2012. ANSOFT will bring forward multiple reports, which will be constantly renewed by the member countries, regarding environmental issues, plant and landscape protection techniques, regulations and policies of each country’s government on an organic ag끼culture， and natural resources such as organic seeds and biological agents.

Bovine embryos produced by in vitro maturation, feretilization and development was examined for presevation and transfer. The fertilization medium used BO medium with 5 mM/ caffeine and 10/ heparin and adjusted to a pH of 7.2 to 7.4. The final concentration of spermatozoa was adjusted to 1 cells/ motile sperm during fertilization in vitro. At 8~10 hrs after insemination, the oocytes were transferred into CR1aa medium and cultured for 7 days. Embryos were preserved by vitrification method for transfer. When the embryos of early, blastocyst and expanded blastocyst stages were frozen-thawed, the proportions of embryos with normal morphology 83.6, 88.1 and 85.2%. (중략)

DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. DNA methylation is a highly plastic and critical component of mammalian development The DNA methyltransferases (Dnmts) are responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. The maintenance DNA methyltransferase enzyme, Dnmt 1, and the de novo methyltransferase, Dnmt3a and Dnmt3b, are indispensable for development because mice homozygous for the targeted disruption of any of these genes are not viable. The occurrence of DNA methylation is not random, and it can result in gene silencing The mechanisms underlying these processes are poorly understood. It is well established that DNA methylation and histone deacetylation operate along a common mechanistic pathway to repress transcription through the action of methyl-binding domain proteins (MBDs), which are components of, or recruit, histone deacetylase (HDAC) complexes to methylated DNA. As a basis for future studies on the role of the DNA-methyl-transferase in porcine development, we have isolated and characterized a partial cDNA coding for the porcine Dnmt1. Total RNA of testis, lung and ovary was isolated with TRlzol according to the manufacture's specifications. 5 ug of total RNA was reverse transcribed with Super Script II in the presence of porcine Dnmt 1 specific primers. Standard PCRs were performed in a total volume of 50 ul with cDNA as template. Two DNA fragmenets in different position were produced about 700bp, 1500bp and were cloned into pCR II-TOPO according to the manufacture's specification. Assembly of all sequences resulted in a cDNA from 158bp of 5'to 4861bp of 3'compare with the known human maintenance methyltransferase. Now, we are cloning the unknown Dnmt 1 region by 5'-RACE method and expression of Dnmt 1 in tissues from adult porcine animals.

The object of this study is to determine the effect of priming on the germination ability and seedling emergence of aged soybean seeds in lab and field conditions. Artificial or natural procedure for seed aging was applied in this study. One seed lot was artificially aged for 3 to 5 days at 42~circC with high relative humidity (nearly RH 100%), and the other one was stored at room temperature for 17 months. Aged seeds were osmoconditioned in -1.1 MPa polyethylene glycol 8000 (PEG) solution for 3 days at 25~circC and air-dried. When Danyeobkong was aged for 4 days average germination was 61.5%, however, this improved to 98.5% after the priming treatment. Improvement of seed germination by priming the aged seed was consistent with large seed sized Jangyeobkong cultivar, indicating that the priming was effective in enhancing seed germinability regardless of seed size. Priming aged seeds also resulted in good stand establishment in the field trials. Germination of aged seeds of Danyeobkong without priming was 17.0%, whereas that of primed ones was 66.4%