We studied the infection rate of and various metacercariocidal approaches to controlling Gymnophalloides seoi for prevention of human infection in cultured and natural oysters in Korea. The selected survey areas were Aphae-do (Shinan-gun, Jeollanam-do), which is an endemic area for G. seoi, and Tongyeong (Geonsangnam-do), which is the main production area of oysters in Korea. In the Tongyeong area, the metacercariae of G. seoi were not detected in cultured oysters (0/201) or wild oysters (0/134). Seventy-two G. seoi metacercariae were observed in 33 of 265 natural oysters collected from Aphae-do; however, metacercariae were not detected in the cultured oysters (0/1101) purchased from the Daejeon Fish Market. To investigate the viability of G. seoi metacercariae, various metacercariocidal treatments were used with 3.5% saline and oyster juice used as positive controls. The metacercariae survived for 75.4 h in 3.5% saline and 112.6 h in oyster juice. After the metacercariocidal treatment, G. seoi metacercariae were survived for 13.29 min in tap water, < 20 sec in 4.3% vinegar, no effect in a rinse of the whole oyster body in 70°C water for 1 sec, but 1 sec in a rinse of the whole oyster body in 90°C water for 1 sec. The greatest metacercariocidal effect on G. seoi was from rinsing oysters in 90°C water followed by those from treatment with 20% ethyl alcohol, 4.3% vinegar, and tap water. However, we suggest that the most actual prevention to G. seoi human infection is rinsing the oysters with tap water for at least 30 min.

판 논문은 구조뭄의 동역학 및 열단성 연성문제 해석을 위 한 통합된 유한요소법을 개 발하논데 초접을 누고 있다. 첫 째로. 열전도 방정식이l 열변위라는 묵리량을 도엽하여 풍역학의 운동 빙정식파 유사하도록 유도한 후. 변분법과 일반좌표계를 이용하여 시간영역에서 정식화하였다 둘째포， 두 뱅정식어] 라블라스 변 판을 동시에 도 입하고 - 공간변수안을 갖는 형성함수짜 가증잔여번을 적용하여 유한요소식을 변환영역에서 표 헌 하였다. 연성된 빙정 식 을 운지l 의 특성에 따라서 분류하였고 정식화 파정을 검숭하였다 또한 수치해석 알고리 늠 이 갖는 수치 역 변환의 정성적인 경향에 대하여 검토하였다-

The removal of nitrogen compounds from a wastewater is essential and it is often accomplished by biological process. An aerobic nitrate-removing bacterium was isolated from a municipal sewage treatment plant and soil. On the basis of its morphological, cultural and physiological characteristics and 16S rRNA sequencing data, this strain was identified as Pseudomonas fluorescens, and named as P. fluorescens K4. The optimal conditions of the initial pH and temperature of media for its growth were 7.0~8.0 and 30℃, respectively. P. fluorescens K4 was able to remove 99.9% of nitrate after 24 h in a culture. The strain could grow with a nitrate concentration up to 800 mg/l and was able to remove 99.9% of nitrate after 104 h of incubation. The optimal electron donor was sodium citrate for a nitrate removal. The strain K4 showed a capability of a complete nitrate removal when the initial C/N ratio was 1.0. An effect of the initial seed concentration was observed for a cell of 10% (v/v) for a nitrate removal. Especially P. fluorescens K4 could completely remove 200 mg/l ammonium for 3 days.