It is remarkable that nuclear transfer using differentiated donor cells can produce physiologically normal cloned animals, but the process is inefficient and highly prone to epigenetic errors. Aberrant patterns of gene expression in clones contribute to the cumulative losses and abnormal phenotypes observed throughout development. Any long lasting effects from cloning, as revealed in some mouse studies, need to be comprehensively evaluated in cloned livestock. These issues raise animal welfare concerns that currently limit the acceptability and applicability of the technology. It is expected that improved reprogramming of the donor genome will increase cloning efficiencies realising a wide range of new agricultural and medical opportunities. Efficient cloning potentially enables rapid dissemination of elite genotypes from nucleus herds to commercial producers. Initial commercialization will, however, focus on producing small numbers of high value animals for natural breeding especially clones of progeny-tested sires, The continual advances in animal genomics towards the identification of genes that influence livestock production traits and human health increase the ability to genetically modify animals to enhance agricultural efficiency and produce superior quality food and biomedical products for niche markets. The potential opportunities in animal agriculture are more challenging than those in biomedicine as they require greater biological efficiency at reduced cost to be economically viable and because of the more difficult consumer acceptance issues. Nevertheless, cloning and transgenesis are being used together to increase the genetic merit of livestock; however, the integration of this technology into farming systems remains some distance in the future.

본 연구는 가축분 종류 및 시용량이 선학초의 생육, 경엽수량 및 개화반응에 미치는 영향을 구명하여 적정 시용량을 알아보기 위하여 실시하였다. 가축분 종류는 우분, 돈분 및 계분으로 하고 시용량은 10a당 각각 1,000, 2,000, 3,000 kg을 시용하여 시험한 결과를 요약하면 다음과 같다. 1. 시험후 토양특성은 P2O5, K는 우분, 돈분, 계분 순으로 높은 경향이었으며 계분 2,000 kg/10a이상시 용구에서는 적정 인산함량보다 높았다. 2. 주요생육은 우분, 돈분 및 계분 모두 2,000 kg/10a 처리구에서 가장 많았으며, 특히 계분2,000 kg/10a에서 가축분 종류간에는 우분〈 돈분〈 계분 순으로 높았다. 3. 전초수량은 돈분 및 계분 2,000 kg/10a 처리구에서 무시용 209 kg/10a에 비하여 각각 82%, 98% 증수되었다 4. 종자의 건물수량은 가축분 종류간에는 우분〈돈분〈 계분 순이었고, 시용량간에는 무처리구 1,000〈 3,000〈 2,000 kg/10a〉순으로 증수되 었다. 5. 개화기는 모든 가축분에서 시용량이 증가할수록 빨라지 는 경향이었고 종실수량은 우분〈 돈분〈 계분 순이었고, 가축분에 관계없이 2,000 kg/10a에서 가장 많았다.

The optimum dosage of quicklime in producing organic fertilizer using livestock wastes with a greater than 80% water content was analysed. After one day had elapsed to allow for the organic fertilizer to dry, the quicklime dosage and the composition of the organic fertilizer were analysed. Any from done to the organic fertilizer was also assessed. The amount of the quicklime required to stabilize livestock wastes was determined by water content of livestock wastes. For J farm(slurry style) of which livestock wastes have 94.6% of water concentration, less than 3% of total amount of livestock wastes, for H farm(scraper style) of which livestock wastes have 85% of water concentration, less then 4% of total livestock wastes and Y farm(traditional style) of which livestock wastes have 80% of water concentration, less then 5% of total livestock wastes. Generally, in order to pack the organic fertilizer, water containing quicklime-stabilized livestock wastes should be less than 35%. It takes 9 days to keep this water content for the wastes from H and Y farms(less than 85% in water content), and 12days for the wastes from J farm(94.6% in water content). According to the classification standard for compost constitution by Higgins, the crude fertilizers from all 3 farms had high grade K2O and CaO, the middle grade T-N and middle or low grade P2O5. Stabilization by quicklime is known to inhibit bacterial decomposition of organic matter and the activity of pathogenic organisms. In this study, more than 99.99% of coliform group, fecal group and viable cell count were reduced. Our results indicate that livestock wastes of greater 80% water content could be used to produce organic fertilizer without the addition of a material for moisture control.

Runoff and water quality was monitored from a watershed with small-scale livestock production farms. To evaluate pollution potential, land use, population, the size of livestock production of each farm, and livestock management method were surveyed. Climate and stream flow data were measured. Water samples were taken periodically for base flow conditions and some storm events. Pollutant loading was estimated by flow volume and concentrations of constituents. Delivery ratio of pollutant load was determined using estimated pollutant load.

The purpose of this study were to make a comparison with the level of environmental consciousness between the livestock farmer and the reference groups( peasant, office worker, housewife and student) and to identify the variables which have an influence on the level of their role performance for environmental protection & preservation. Data for this study were collected from 822 cases including the livestock farmer(109), the peasant(67), the office worker(140), the housewife(119) and the student(356). The SPSS software program and statistics such as frequencies, x2, ANOVA, multiple regression analysis, Chronbach's α and factor analysis were used to analyze the data for this study. The major findings of this study were as follows: 1. The pro-environmental attitude score(environmental sensitivity and attitude of environmental protection & preservation) the groups of office worker and housewife is shown higher than the groups of livestock farmer, peasant and student. 2. The consciousness of environmental problem is relatively low to the group of the livestock farmer compared with the reference groups. 3. As a result of multiple regression analysis, four determinants such as locus of control, lifestyle, environmental sensitivity, and knowledge of environmental issues explained 38.8% of the livestock farmer's role performance for the environmental protection & preservation.

Buffer zone selection technique for natural purification of livestock wastewater within a small agricultural watershed was developed using Geographic Information Systems. The technique was applied to 4.12 km2 watershed located in Gosan-myun, Ansung-gun which have 20 livestock farmhouses. As a necessary data for selecting process, feedlot site map, digital Elevation Model (DEM), stream network, soil and land use map were prepared. By using these data, wastewater moving-path tracing program from each feedlot to the stream was developed to get the basic topographic factors; average slope through the paths, distance to the nearest stream and watershed outlet. To identify the vulnerable feedlots for storm event, the grid-based storm runoff model (Kim, 1998; Kim et al., 1998) was adopted. The result helps to narrow down the suitable area of buffer zone, and finally by using subjective but persuasive conditions related to elevation, slope and land use, the suitable buffer zones were selected.

The sewage sludges and livestock manures, respectively, were composted with sawdust used for control moisture in the static piles system composter. The variations of temperature, pH, moisture, C/N ratio, inorganic content, forms of organic materials and nitrogen, and contents of heavy metals were investigated. The results were summarized as follows ; The temperature for composting the sewage sludges reached the highest temperature of 52℃, after 3 days and lasted for 7 days, and then went down 30℃ after 52 days. In the case of composting livestock manures, the temperature reached to 63℃ after 10 days, that lasted for 10 days, and then went down gradually. After upsetting the sewage sludges and livestock manures, the temperature went up but was little changed after 52 days. Thus we decided that the terminal of composting periods would be 52 days. The moisture contents of the sludges and livestock manures for composting were decreased to 30% and 36%, respectively. The contents of inorganic matters and heavy metals were changed by the characteristics of raw materials but increased gradually during composting process. The total contents of organic materials in the sewage sludges and livestock manures for composting were decreased to 7% and 9%, respectively. The contents of ether extracts, resins, hemicellulose and cellulose were decreased but those of water soluble polysaccharides and lignins were not changed. The total contents of nitrogen in sewage sludges and livestock manures were decreased to 43% and 34%, respectively.

This study was conducted to investigate the removal characteristics of PO4 3- -P contained in livestock wastewater using waste concrete. With small particle size, increased dosage and temperature of water, PO4 3- -P was well removed by waste concrete. PO4 3- -P was removed by adsorption reaction in low pH of the primary phase, but the crystallization reaction predominated for increasing pH with passed time. As a result of adapting the adsorption isotherm equation, PO4 3- -P removal was more affected by the crystallization reaction than the adsorption reaction. In the SEM micrograph, there was no evident change on the waste concrete surface. Particle size was plate-phase before reaction but appeared a dense form to progress in the crystallization reaction after reaction.

Environmental impact assessment survey reflecting farmers` opinion on the residence and production space in rural settlement area by ORD showed that more than 86% of respondents thought their reservoirs and waterways (small rivers) were getting seriously contaminated primarily by garbage and livestock manure. A typical rural settlement unit was taken to assess the impact of improper management of livestock manure in the farms on the water quality of small river flowing down along the villages where swine and dairy farms were situated in Daejook 2, 3-ri, Seolseong-myun, Icheon-gun. Nitrogen compounds such as NO3-N, NO2-N, NH3-N, and phosphorus compound HxPO4, DO, BOD5, COD, and microbial density were analyzed to evaluate water quality at five test sites designated along the water stream. Tests showed. for example, BOD5 at site 4 was average 9.2mg/l which was about 3~8 times higher than that of observation site 2 and 3, at which most livestock houses were situated. This is a clear evidence that the nutrients of livestock manure illegally discharged to small river can lead to an eutrophication of the river at downstream. A soil absorption system with aeration could be one of alternatives to treat the contaminated wastewater by livestock manure. The place at downstream, inbetween observation site 1 and 2, could be the best construction site for the treatment facility from the standpoint of the overall treatment efficiency, An enclosed composting system can also be regarded as a good alternative for treatment of the sludge which is the by-product of the soil absorption system operation.

This is a case study on the counterplan for reducing agricultural environmental pollution in the SANHYUN Livestock Club through participatory research from March, 1993 to June, 1995. In the monthly meetings, the active discussion about agricultural environmental pollution gradually increased with the village people, Club members and other people because of a consciousness-raising created through discussion activities. In addition, the Club members have established a septic tank and accumulation store-house after active participation in such discussions about the environmental pollution. After two years, the number of septic tanks and accumulation store-houses has increased from two to 10. Gradually all village people are becoming more aware of village environmental pollution. Four non-member farmers have constructed a septic tanks and several member farmers have constructed the fermentation system of sawdust and hulls.

The actual condition of farmstead, mainly livestock facility, was surveyed in ChungChongBukDo in order to find out factors and problems of farmstead planning design. From the data collected, a type, plot, size and scope of farmstead, relationship of house and farmstead, topography, climate, proprietor's opinion on them were analyzed or obtained as the basic design criteria for planning: designs. The best type of farmsteads was individual farm having livestock facilities. They were mostly breeding a kind of livestock without their plan and Bone planning at residing area. The south aspect of the facility was extremely much and slope of the land was mostly 15。 below. Wind blows from East-South in summer and winter wind are West-North extremely much. Sun heat was mostly above 6 hours. In the case of collective and composite farmstead, proprietors wanted that a head of cow has 1,000-3,000 pyeong, the area of facility has 1,500-2,000 pyeong, total area of farmstead has 45,000-400,000 pyeong and an estimated expense is 0.2 - 1.5 billion won. Farmstead planning design was proposed through field survey results and contents of'references. Examples of farmstead planning design were composite and concentrated type and existed according to the situation of road(North, South, East, and West).