물레나물 약유래 캘러스로부터 신초 재분화에 미치는 저온 전처리 및 식물생장조절물질의 효과를 조사하기 약배양을 실시하였다. 약유래 캘러스 유도 및 식물체 재분화에 미치는 식물생장조절제의 효과를 조사하기 위해 2,4-D, NAA, BA 및 TDZ를 8종류로 혼합한 1/2 MS 배지에 약배양을 실시한 결과 물레나물은 식물생장소절물질에 의존적인 식물로 배지내 식물생장조절물질이 첨가되지 않으면 캘러스가 형성되지 않았다. 2,4-D가 포함된 배지에서 약유래 캘러스 형성이 양호하였으며 2.0mg/L 9,4-D단용 처리에서 캘러스 형성율이 52.6%로 가장 높았으며 지속적 캘러스의 증식 및 shoot 분화는 0.1mg/L 2,4-D와 1.0mg/L BA 혼용처리가 효과적이었다. 2.0mg/L 2,4-D 단용처리에 의해 형성된 캘러스로부터 부정적 다수의 신초분화에 미치는 TDZ와 BA 효과를 조사한 결과 1.0mg/L TDZ 처리구에서 단 한개의 shoot가 분화된 것을 제외하고는 갤러스가 갈변되어 효과적이지 못하였으나 모든 BA 처리구에서 갤러스는 shoot가 분화되어 TDZ에 비해 효과적이었다. 특히 3.0mg/L BA 처리구에서는 캘러스 모두 shoo4로 분화되어 다수의 multishoot가 형성되어 가장 효과적이었다. 개화 1주일전의 화뢰를 5℃ 에서 8일간, 15일간 저온 전처리 하여 0.1mg/L 2,4-D와 1.0 mg/L BA 혼용처리된 DIS 배지에 배양한 결과 무처리에 비해 처기구에서 약의 생육기간이 길었으며 특히 5℃ 15일간 저온처리한 약이 배양기간 동안 지속적으로 캘러스 및 부정 shoot를 형성하여 저온처리 효과가 있었다.

This experiments were carried out to find out the effects of different explant materials, kinds and concentration of plant growth regulators, and total nitrogen and sucrose contents on the in vitro regeneration of Abeliophyllum distichum Nakai. The effects of growth regulators on regeneration from 3 explant sources (leaf, internode and node) were more or less same. Leaf explants produced only callus with 2ip (Isopentenyladenine) and NAA (Naphthaleneacetic acid) treatment and other regulators had no effects. Test with internode explants yielded about same results but callus was obtained with 2,4-D (2,4-Dichlorophenoxyacetic acid). Node explants resulted in shoot regeneration by all regulator treatment except NAA and 2,4-D, but control also showed similar results. Callus formation from internode and node explants was vigorous by 2ip, zeatin, and 2,4-D treatments and high NAA concentration resulted in higher callus formation. In this experiment, various mixed treatment of growth regulators were also employed, using node as explant material. Shoot regeneration was obtained with BA (Benzyl adenine) + NAA treatments but the results were comparable with control. Generally shoot and root regeneration was poor with all combined treatment except 2ip + NAA and 2,4-D + NAA. However, callus was formed readily with all treatments. In this experiment, combined treatments of regulators were applied on the callus derived from singular regulator treatment. The results showed no shoot and root regeneration with any combination of 2,4-D, IAA (Indoleacetic acid) and NAA, but soft milky white callus was formed in all the treatments. No shoot and root regeneration was observed with any combination of 2iP, NAA and IAA, but somewhat hard, light green callus was formed in all the treatments. Callus formation decreased with high kinetin concentration in case of kinetin + NAA treatment. The experiments with total nitrogen content of media showed that low concentrations of 15 and 30mM were effective for the shoot and root regeneration. Sucrose experiment demonstrated shoot regeneration with 1~4% concentration, and root and callus formation with 2~4%. No root and callus formation was observed with 0 and 1% sucrose.

노지에 있는 교잡종 블랙베리의 근맹아를 채취하여 1.2% sodium hypochlorite 용액에 침지하여 표면 살균한 후 BA를 첨가한 변형된 × 1, × 1/2 MS 고체배지에서 다경유도를 시도하였다. 4주간 배양하였을 때 BA 1mg·L-1가 첨가된 기본 MS배지에서 고빈도(83.3%)의 다경유도가 이루어 졌으며, 절편체당 신초수는 0.5mg·L-1 BA를 첨가한 MS 기본배지에서 3.7개로 가장 높게 형성되었다. 또한 12주간 배양하였을 때 4주간 한 경우에 비하여 신초형성율이 4.1배 많은 15.4개가 형성됨을 관찰할 수 있었다. 형성된 신초로부터 뿌리발생은 식물생장조절물질이 첨가되지 않은 MS배지를 1/1, 1/2, 1/4배로 희석하여 신초를 배양하였을 때 특히 1/2 MS 배지에서 95%로 가장 양호하였다. 재생된 식물체는 모래 : 원예용상토 : 버미큘라이트(1:1:1, vol.)혼합토양에서 순화하였을 경우 95%의 생존율을 나타내었다.

An efficient somatic embryogenesis and plant regeneration protocol was developed for Schisandra chinensis Baill, using embryogenic cell suspensions and optimized media conditions. Friable embryogenic callus was induced from cotyledonary leaf and hypocotyl explants of 7 days old seedlings on MS agar medium supplemented with 1.0 to 4.0 mg l-1 of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast growing and well dispersed embryogenic cell suspensions were developed within two months when embryogenic calli were transferred to MS liquid medium containing 1.0 mg l-1 2,4-D. One third strength of MS medium was the best for both overall growth and development of somatic embryos in liquid culture. Over 3400 viable somatic embryos were produced from each 150 ml flask with an initial cell density of 30 mg in 30 ml medium. Germinated somatic embryos developed in liquid medium converted into plantlets after transferred to half-strength MS semi-solid medium. Approximately 90% of the converted plantlets were successfully transplanted to soil and grew into fertile plants.

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

In general, since partial pollen is derived to sporophyte, anther culture efficiency is low, and practical application that is introduced in actuality breeding technology is not many. This study was carried out to improve regeneration of green plants through some culture environments contol in anther culture of naked brley. Among the factors related with plant regeneration, medium was effective in component containing L-glutamine 256 mg/L, L-proline 250 mg/L, IAA 1 mg/L and BAP 2 mg/L controlled in addition sucrose 30 g/L or maltose and sucrose mixed each 30 g/L to increase both plant regeneration and green plant regeneration rate. Also, adequate content of CuSO4 was the best at 1.25 mg/L (fifty-fold), it was tendency to decrease albino production rate. Starvation was effective at 30℃, 7 days in case of Saessalbori for plant regeneration and Dooweonchapssalbori at 30℃, 10 days with increasing green plant regeneration against albino. After plant regeneration, under acclimation by hydroponics, roots and shoots were well developed at 20℃, light control as 2,000～5,000 Lux and photoperiodic reaction by 14/10 as dark/light in the early growth stage. In acclimation method, plants acclimated in the modified Yoshida solution filled-vermiculite in GP pot is superior (100%), in which was controlled by temperature 20℃, pH 6.0 and relative humid 90% over, especially, after transplanting in pot growth of root, sheath and leaf are more active in 20℃ and 5,000 Lux control. For Vernalization, plants derived from anther culture of F1 naked barley was different from their parents with normal heading plant even about 50% in the F1 hybrids whose vernalization was strong, whereas the rest of plants derived from anther culture formed rosette, showing that normal growth were impossible.