Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20~40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at 8℃ and for 15 days at 6℃ without decrease in hatchability and larval survivorship, respectively.
Maggot therapy (MT) has been re-introduced for non-healing wounds such as pressure sore, diabetic and necrotic ulcers, as well as infected surgical wounds, burn, and trauma injuries since early 1990s. For the production of sterile maggot of proper developmental stage, Lucilia sericata were studied on developmental periods of immature stages on liver agar medium under six different temperatures such as 15.4, 20.6, 22.5, 26.2, 29.1, and 33.0℃, and adult longevity and egg production under 29℃. The periods of eggs and larvae of the I st and the 2nd instars were shortest at 33℃ by 9.0, 14.0 and 18.6 hours, respectively. The periods of the 3rd instar larvae and pupae were shortest at 29.1℃ by 285.0 and 171.0 hours, respectively. Developmental zero point and total effective temperature for the development of each stage were calculated based on the developmental periods. Adults of both female and male lived more than one month. Females laid an average of 338.5 eggs through 2.7 times of egg laying throughout her lifetime.