The present study was evaluated the antibacterial effect of the combination of Coptidis rhizoma, Glycyrrhiza uralensis Fischet, Schizandra chinensis and Corni Fructus(1:1:1) extracts(CGSC10). Furthermore, the effectiveness of CGSC10, sodium chlorate, and the combination of CGSC10 and sodium chlorate(CGSCS10) against E. coli O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 5, 10, and 20% CGSC10 was inhibited the growth of E. coli O157:H7 by 34.7, 60.2, and 76.4%, respectively. For 7 days after single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were administered in drinking water with saline, 10% CGSC10, 15 mM sodium chlorate, and CGSCS10, respectively. On the 3rd day, the number of E. coli O157:H7 in mouse feces was significantly decreased by administration of CGSC10, 15 mM sodium chlorate, and CGSCS10 (p < 0.001). On the 7th day-after administration, CGSC10, sodium chlorate, and CGSCS10 were decreased the number of E. coli O157:H7 by 27.1, 67.7, and 83.3%, respectively. According to the results of the present study, administration of CGSCS10 to mice can reduce the severity of E. coli O157:H7 infection. In addition, it is suggested that CGSCS10 represents a good candidate for the treatment of enteric infections in domestic animals.

The present study was investigated the antibacterial effect of several sodium salts and the sodium salt mixture composed with sodium chlorate, sodium azide and sodium cyanide on Salmonella gallinarum (S. gallinarum) infection in murine derived macrophage RAW 264.7 cells. In the infection assay of S. gallinarum in macrophage cells pretreated with 15 mM sodium chlorate, 0.3 mM sodium cyanide, 0.3 mM sodium azide and the sodium salt mixture (15 mM sodium chlorate, 0.3 mM sodium cyanide, 0.3 mM sodium azide), respectively, the numbers of S. gallinarum in all treated-groups tended to decrease in the process of time after treatment, but the group treated with sodium cyanide was no significant difference compared with control. After 24 hours of treatment, the number of S. gallinarum in sodium azide (p<0.05), sodium chlorate (p<0.001) and the sodium salt mixture (p<0.001) treated-group was significantly decreased compared with control, and that in the sodium salt mixture treated-group was decreased the higher than all groups. The results of this study demonstrated that the sodium salt mixture composed with sodium chlorate, sodium azide and sodium cyanide, has the antimicrobial activity for S. gallinarum and may be beneficial on the control of intracellular pathogens.

충남 논산시, 부여군 시설딸기재배지에서 2010년 4∼5월에 40개의 포장의 토양 을 채집하여 식물기생선충을 조사한 결과 뿌리혹선충과 뿌리썩이선충이 우점선충 이었으며 각각 32.5%, 52.5%의 검출율을 보였으며, 평균 밀도는 토양 100g당 뿌 리혹선충은 49마리(J2), 뿌리썩이선충은 328마리였다. 이중 뿌리혹선충과 뿌리썩 이선충이 동시에 감염된 포장은 11개포장으로 27.5%를 보였다. 뿌리혹선충과 뿌 리썩이선충이 동시에 감염된 유기농 딸기 포장의 선충 밀도는 정식시(2009. 9. 7.) 뿌리혹선충과 뿌리썩이선충은 각각 24, 23마리/토양100g에서 5개월후에는 25.8 배(629마리) 24.3배(559마리) 증가하여 피해를 주었다. 두 선충이 감염된 포장의 딸기는 태양열 토양 소독으로 선충을 방제한 포장의 딸기에 비해 지상부 생육과 뿌리의 발달이 저조하였다.