Spinach (Spinacia oleracea L.), a green leafy vegetable, is well known as a functional food due to its biological activities. Vascular calcification is associated with several disease conditions including atherosclerosis, diabetes, and chronic kidney disease (CKD), and is known to raise the risk of cardiovascular diseases related morbidity and mortality. However, there are no previous studies that have investigated the effects of fermented spinach exract (FSE) against aortic and its underlying mechanisms. Therefore, this study investigated the effects and action of possible mechanisms of FSE on inorganic phosphate (PI)-induced vascular calcification in ex vivo mouse aortic rings. PI increased vascular calcification through calcium deposition in ex vivo aortic rings. FSE inhibited calcium accumulation and osteogenic key marker, runt-related transcription factor 2 (Runx2), and bone Morphogenetic Protein 2 (BMP-2) protein expression in ex vivo aortic rings. And, FSE inhibited PI-induced extracellular signal-regulated kinase (ERK) and p38 phosphorylation in ex vivo aortic rings. These results show that FSE can prevent vascular calcification which may be a crucial way for the prevention and treatment of vascular disease association with vascular calcification.

본 논문에서는 유도무기 통합 설계 프레임워크를 위한 데이터베이스를 설계하였다. 실제 개발에 앞서 유도무기 설계에 필요한 데이터베이스를 위한 요구도를 분석하였으며, 데이터베이스 설계 개념을 정립하였다. 또한 해석도구 간의 데이터흐름 등을 파악하였으며, 이들 정보를 토대로 테이블들을 구성하고 각 객체관계를 정의하여 데이터베이스 스키마를 설계 및 구축하였다. 개발된 DB는 유도무기 통합 설계 프레임워크에 통합되었으며 프레임워크내의 데이터 저장, 검색 및 관리 등의 역할을 수행하며, 유도무기 설계에 적용하여 그 효용성을 검증하였다.

In this study, the relation of the preservation efficacy reduction with methyl paraben of parahydroxybenzoic acid derivatives was investigated using the dialysis membrane method with tween-80 of surfactant and TiO2/Talc of inorganic powder meterial from emulsion system. It was found that the preservation efficacy of tween-80 and TiO2/Talc from emulsion system was reduced due to the adsorption of methyl paraben. According to the microbe test, In case of tween-80, MBC appeared in 0.19 w/v% and in case of TiO2/Talc, MBC appeared in 0.22w/v% / 0.23w/v%. In general, the equation of Talc's adsorption weight(A·W) has a tendency to show in A·W=11.5C0.745