알락수염노린재의 주요 페로몬 성분을 찾기 위하여 알락수염노린재 헥산추출물에 대하여 GC-MS 및 GC-EAD 반응을 분석하였고, GC-EAD에 반응한 물질에 대하여 Y-tube Olfactometer로 알락수염노린재의 유인반응을 조사하였다. 알락수염노린재의 주요 헥산추출물은 (E)-2-hexenal, 4-Oxo-(E)-2-hexenal, (E)-2-hexenyl acetate, (E)-2-octenal, n-undecane, n-dodecane, (E)-2- decenal, n-tridecane, (E) and (Z)-2-decenyl acetate, pentadecane, tetradecyl isobutanoate, (Z)-9-octadecenoic acid methyl ester 및 기타 미동정 물질 등이 다수 존재하였다. 특히 암컷 알락수염노린재에는 수컷 노린재에는 없는 E-5-decenyl acetate의 함량이 매우 많았다. 알락수염노린재 헥산추출물에 대한 알락수염노린재 암․수의 안테나 반응은 retention times 4.1～ 4.2(retention index 850～860 : E-2-hexenal), 4.7 부근(retention index 960 : 4-oxo-E-2-hexenal)과 6.8 부근(retention index 1290～1305 : Tridecane)의 화합물에 대해 암․수 모두 동일한 안테나 반응을 보였다. 알락수염노린재에 대한 Y-tube Ocfactometer 시험에서 알락수염노린재의 최초 이동방향이 수컷은 E-2-decenal(100%), E,E,Z-2,4,6-decatrienoate(80%), n-tridecane (70%) 및 E-2-hexenal(60%) 순이었고, 암컷은 n-tridecane(80%), 4-oxo-E-2-hexenal (60%), E-2-decenyl acetate(60%), Z-2-decenyl acetate(60%), E,E,Z-2,4,6-decatrienoate (60%) 등의 순이었다. 특히 Tridecane과 갈색날개노린재 집합페로몬인 E,E,Z-2,4,6-decatrienoate는 알락수염노린재 암․수 모두에서 높은 유인효과를 나타내었다.

It is well known that the membrane permeation in pervaporation is governed by both the chemical nature of the membrane material and the physical structure of the membrane and also the separation can be achieved by differences in either solubility, size or shape. The solubility of the penerrant in the polymeric membrane can be described qualitively by applying the Hildebrand relation [1] which relates the energy of mixing of the penerrant and the polymer material. Froehling et al. have tried to predict the swelling behavior of polymers for the systems of polyvinylchloride(PVC)-toluene-methanol, PVC-trichloroethylene-nitromethane and PVC-n-butylacetate-nitromethane[2]. The former two systems which do not show the donor/acceptor interactions upon mixing showed the successful results[2]. In addition to this technique, there are several other possible approaches to predict the swelling behaviors of polymers, such as the surface thermodynamic approach[3, 4], the comparison of the membrane polarity with the solvent polarity in terms of Dimroth's solvent polarity value[5].

피부 기저막(basement membrane, BM)이란 표피와 진피 사이에 존재하는 특별한 구조물로 표피와 진피 를 단단히 고정시켜 피부 구조를 유지하는 데에 중요한 역할을 수행한다. 노화 및 자외선 노출에 의한 피부 기저막 의 구조적 변화와 파괴는 피부 주름 형성과 탄력 저하를 포함하는 피부노화 현상의 요인으로 여겨지고 있다. Laminin-332 (LN-332)는 피부 기저막을 구성하는 주성분으로 피부에서 표피와 진피를 단단히 고정시키는데 중요한 역할을 한다. 본 연구에서는 왕불유행 헥산 분획물(Melandrium firmum hexane fraction, MFHF)이 각 질형성세포에서 LN-332 발현에 미치는 효과를 확인하였다. 정량적 real-time PCR (RT-PCR)과 단백질 발현 분석을 통해서 MFHF가 LN-332의 mRNA 발현 및 단백질 발현을 촉진시키는 것을 확인하였다. 또한 MFHF가 어떤 신호전달 경로를 통해 LN-332 발현을 조절하는지 확인하기 위하여 p38 MAPK 억제제인 SB202190과 ERK1/2 억제제인 U0126을 처리한 결과, p38 MAPK 억제제에 의해서 LN-332 발현이 완벽히 억제됨을 확인 하였다. 또한, 피부 기저막을 구성하고 있는 콜라겐 타입 VII과 integrin α6의 mRNA 발현 역시 MFHF에 의해 증가하는 것을 확인하였다. 우리는 본 연구를 통해 MFHF가 각질형성세포에 작용하여 피부 기저막을 구성하는 성분들의 생성을 촉진할 수 있는 소재로 작용할 수 있다는 것을 확인하였다. 이러한 결과는 기저막의 구조적, 기능 적 이상에 의해 나타나는 피부노화 현상의 개선을 위해 활용할 수 있을 것이라 제안한다.

This study was conducted to improve the analytical method of siloxanes in biogas. Methanol and hexane were tested as absorption solvents of the impinger absorption method, and also the hexane extraction for pretreatment of sample was evaluated. Manufactured gas contained siloxanes of 50 ppm was completely absorbed by the methanol impinger absorption. The absorption efficiency of biogas containing only 2 ppm, however, was maximum 84%. As the condensate on the first impinger increased, the absorption rate of methanol was decreased. The hexane extraction method of the sample was considered to proper the method of moisture removal. The hexane extraction result showed the high recovery factor and the low relative standard deviation. It is suggested that the suitable choice of solvent and pretreatment is required, as the analysis result of siloxane sample may be differentiated depending on the type of biogas or the sampling point.

Present study evaluated the low-temperature destruction of n-hexane and benzene using mesh-type transition-metal platinum(Pt)/stainless steel(SS) catalyst. The parameters tested for the evaluation of catalytic destruction efficiencies of the two volatile organic compounds(VOC) included input concentration, reaction time, reaction temperature, and surface area of catalyst. It was found that the input concentration affected the destruction efficiencies of n-hexane and benzene, but that this input-concentration effect depended upon VOC type. The destruction efficiencies increased as the reaction time increased, but they were similar between two reaction times for benzene(50 and 60 sec), thereby suggesting that high temperatures are not always proper for thermal destruction of VOCs, when considering the destruction efficiency and operation costs of thermal catalytic system together. Similar to the effects of the input concentration on destruction efficiency of VOCs, the reaction temperature influenced the destruction efficiencies of n-hexane and benzene, but this temperature effect depended upon VOC type. As expected, the destruction efficiencies of n-hexane increased as the surface area of catalyst, but for benzene, the increase rate was not significant, thereby suggesting that similar to the effects of the reaction temperature on destruction efficiency of VOCs, high catalyst surface areas are not always proper for economical thermal destruction of VOCs. Depending upon the inlet concentrations and reaction temperatures, almost 100% of both n-hexane and benzene could be destructed. The current results also suggested that when applying the mesh type transition Metal Pt/SS catalyst for the better catalytic pyrolysis of VOC, VOC type should be considered, along with reaction temperature, surface area of catalyst, reaction time and input concentration.