In this study, Target strength for multi-frequency (38 kHz, 70 kHz, 120 kHz and 200 kHz) of chub mackerel (Scomber japonicus) was estimated using by the KRM model. The body shape of the Chub mackerel was described by an X-ray system and the body length of 20 individuals ranged from 16 cm to 28 cm. The swimbladder tilt angle ranged between – 8 and – 14°, the maximum TS value according to the swimming angle of chub mackerel was – 33.0 dB at – 11°. The averaged TScm according to fork length was – 66.02 dB at 38 kHz, – 66.50 dB at 70 kHz, – 66.00 dB at 120 kHz and – 67.35 dB at 200kHz, respectively.

Rockfish was a commercially important fish specie in marine ranching areas in Korea. To estimate density and biomass of rockfish using acoustic method, target strength (TS) information is required on the species. This study measured TS dependence on tilt angle and size on 14 live rockfish individuals at 38, 70, and, 120 kHz by ex-situ measurement (tethered method) and acoustic scattering model (Krichhoff ray mode, KRM). The swimbladdered angle ranged from 18 to 30˚ (mean ± s.d. = 26 ± 4˚ ). The mean TS for all individuals was highest -35.9 dB of tilt angle -17˚ at 38 kHz, -35.4 dB of tilt angle -25˚ at 70 kHz, and -34.9 dB of tilt angle -22˚ at 120 kHz. The ex-situ TS-total length (TL, cm) relationships were TS38kHz = 20log10(TL) - 67.1, TS70kHz = 20log10(TL) - 68.6, and TS120kHz = 20log10(TL) - 69.9, respectively. The model TS-total length (TL, cm) relationships were TS38kHz = 20log10(TL) - 66.4, TS70kHz = 20log10(TL) - 67.0, TS120kHz = 20log10(TL) - 67.0. The two measurements between the ex-situ TS and KRM model for TS-tilt angle and fish size were found to be significantly correlated.

This study was focused on acoustic scattering characteristics of jack mackerel (Trachurus japonicus) at frequency 38, 70, and 120 kHz by Kirchhoff-ray mode (KRM) model. The body length (BL) of 16 individuals ranged in 12.2~22.0 cm (mean±S.D.: 17.8±3.2 cm) and the swimbladder length ranged in 4.2~8.6 cm (mean±S.D.: 6.6±1.6 cm) and the swimbladder cross section ranged in 1.7~6.6 cm2 (mean±S.D.: 3.8±1.6 cm2). This result shows that results correlate well between the BL and the length and cross section of swimbladder. The swimbladder angle ranged in 7~12°and the maximum TS values ranged in –16~–5°at tilt angle. The averaged TS-to-BL relationship were TS38kHz=20log10BL-65.33 (R2=0.66), TS70kHz=20log10BL-65.90 (R2=0.67), and TS120kHz=20log10BL-66.65 (R2=0.65). These results can be used fundamental data in order to estimate distribution and biomass of jack mackerel by using hydro-acoustic method.

Acoustic target strength (TS) measurement and theoretical acoustic scattering models were applied to estimate the TS for assessing the fish biomass. TS measurement was made of ex situ sailfin sandfish Arctoscopus japonicus at 70 and 120 kHz, and then compared to backscatter model prediction. The live sandfish was tethered in seawater using monofilament lines. Measurements were made versus incidence angle, −50° to 50° relative to dorsal aspect directions. Distorted wave Born approximation (DWBA) model was used to calculated TS pattern. The TS values of sandfish (body length: 16.2 cm) at 70 and 120 kHz were ‒66.94 dB and ‒64.45 dB, respectively, and were about 20 dB lower than TS of other fishes in Korea waters. These TS levels were distributed within the range of the theoretical TS. Ex situ measurement and theoretical TS may be applied to improve acoustical detection and biomass estimation of the sandfish, and is necessary to measure with various lengths.

Acoustical backscattering characteristics of Japanese anchovy can be estimated by Kirchhoffray mode model (KRM model) due to estimate exact body and swim-bladder shape of the fish, the samples were rapidly frozen by dry-ice and alcohol. X-ray photos for ventral and lateral direction for 6 samples were taken and the 3D coordinates of the body swim-bladder were estimated by digitizing from the photos. The angles between the axis of body and swim-bladder were about 9˚ at 38kHz and 7˚ at 120kHz, 200kHz. General formula of TS and BL estimated were 〈 TS38kHz 〉=20logBL-67.3, 〈 TS120kHz 〉=20logBL-66.6, 〈 TS200kHz 〉=20logBL-67.0. As a result, we confirmed KRM model is very useful to estimate TS (Target Strength) for design of experiment and it also can be applied to estimate the abundance of Japanese anchovy distributed by 2 frequency difference method in the survey area.

The green peach aphids, Myzus persicae (Sulzer), feed consistently and regularly throughout all stages of nymphs and adult. That is, honey dew production (HDP), which indicates a state of feeding, occurs at regular intervals within a given stage, and moving (MV), which interrupts feeding, tends to occur scarcely. We used these behaviors to test whether the sound stimuli acted as a stress in this species. We conducted the playback experiment in which acoustic stimuli with a combination of five frequency (100, 500, 1000, 5000, and 10000 Hz) and three intensity levels (66, 78, 90 dB SPL) were presented to all stages of aphids. Under all acoustic stimuli, HDP occurred less often, and MV occurred more often and longer. The results of two-way multivariate ANOVA showed that the stimulus frequency was a significant factor for variation in aphid behaviors in the playback experiment. However, there was no intensity effect on aphid’s behavior. Based on the electrical penetration graph experiment, aphid feeding was frequently stopped and lasted only for short durations under acoustic stimuli. The results of both experiments demonstrated that the acoustic stimuli were effective in inducing feeding suppression in this species. Thus, the acoustic stimuli may be a stressor in the green peach aphid. Furthermore, the behavioral bioassay employed in this study may be used to assess the effect of various stressors on aphids.

Acoustic side-aspect target strength (TS) of living Japanese anchovy (Engraulis japonicus) was measured at 120kHz during in situ experiments. The data were collected by lowering and horizontally projecting the splitbeam transducer into the anchovy school. For analysis and interpretation of the side-aspect TS data, acoustic theoretical model, based on the fish morphology, and dorsal-aspect TS data were used. Total length of the anchovy ranged from 6.6 to 12.8cm (mean length 9.3cm). The side-aspect TS distributed between -40 and -55dB, has an obvious length dependency. The mean side-aspect TS of the anchovy was -47.8dB, and the TS was about 2dB higher than mean TS generated from dorsal-aspect measurements. With reference to maximum TS, the results of the side-aspect TS were distributed within the range of the theoretical and dorsal-aspect TS. Apparently these tendency indicates that side-aspect TS measured from the study is useful data. These in situ measurements of side-aspect TS can be applied to improve acoustic detection and estimates of the anchovy, and is necessary to measure with a various frequency and length for making enhance data.