바퀴는 혐오감을 줄 뿐만 아니라 여러 종류의 병원체를 기계적으로 전파하는 주요한 위생곤충이며, 바퀴 방제를 위해 독먹이제를 가장 많이 사용한다. 본 연구에서는 4종류(chlorpyrifos, avermectin, hydramethylnon 및 fipronil)의 살충 주성분이 각각 함유된 국내 유통 독먹이제 3종 (Korea Combat Ultra Slim (K-CUS), Korea Combat Power (K-CP) 및 Korea Zaps Dual Bait (K-ZDB))과 미국 유통 독먹이제 3종(US Combat Source Kill Max (US-CSKM), US Hot Shot MaxAttrax Roach Bait (US-HSM) 및 US Raid Double Control Small Roach Bait (US-RDC))의 독일바퀴(Blattella germanica)에 대한 살충효력을 검증하였다. 6종의 독먹이제 중 fipronil을 살충 주성분으로 사용하는 세 가지의 독먹이제(K-CUS, K-CP 및 US-CSKM)가 속효성 및 높은 살충효과를 나타내었다. K-CUS과 K-CP의 살충효력의 지속성을 검정하기 위해 두 제품을 개봉 후 6개월 및 12개월 이후에 추가로 생물검정을 실시한 결과, 두 제품 모두 시간이 지남에 따라 제품 개봉 직후에 비해 살충효 과가 감소하는 경향을 보였지만, 40시간 이내 독일바퀴에 대한 사충률이 90%에 도달하는 것을 감안하면 적어도 이들 독먹이제의 옥내 설치 후 1 년동안은 살충효과가 유지되는 것으로 판단된다.

Three formula types (gel, cookie, and liquid), six commercial roach bait products containing one of active ingredients among Fipronil, Avermectin, Dinotefuran, and Chlorpyrifos were examined against susceptible strain of German cockroach, Blattella germanica (L.). For the results of killing speed (LT50 values), universal killing speed effects depended on bait formula types. The killing speed of liquid bait formula type was the fastest among three formula types. Followed by gel bait type and cookie bait type. However the control speed (LT90 values) effects depended on the active ingredients that contained in roach baits. The baits contained Fipronil were shown the strongest control performance against German cockroaches. Chlorpyrifos was similar with Fipronil. Followed by Avermectin and Dinotefuran.

This study was conducted to investigate the attractant substances of different solvent fractions of mackerel and tuna viscera. Mackerel and tuna viscera were extracted with methanol and the resultant were fractionated with n-hexane, chloroform, ethyl acetate, n-butanol and water. The chemical compounds such as free amino acid, fatty acid, nucleotide related compounds contents were estimated for mackerel, tuna viscera and various fractions. These fractions were also subjected to attracting experiments in water tank to estimate attracting effects. The aqueous fractions of mackerel and tuna viscera showed the highest attraction effect on swimming crab. The major chemical compounds of the aqueous fractions were histidine, taurine, cysteine, glutamic acid, inosine monophosphate (IMP) and inosine (HxR) for mackerel and arginine, glutamic acid, aspartic aid, alanine, IMP and hypoxanthin (Hx). Results indicated that higher polarity compounds, such as amino acid and nucleotide related compounds have higher attraction activities than nonpolar substances such as neutral lipid.

This research is aiming to develop generic attractants for lepidopteran pests that can be used as a control tool for environmentally friendly management against the lepidopteran pests in orchards. During the first year of this research we carried out various trapping experiments in apple orchards in Korea. Field trapping experiments were carried out at two apple orchards in UaiSung-Gun, GyungBuk Province during May – September 2010, using different rice wines and fruit-based fermenting baits. Overall, when six major moth pest species(Grapholita molesta, Carposina sasakii, Adoxophyes orana, Choristoneura longicellana, Archippus breviplicanus, Phyllonorycter ringoniella) in apple orchards were counted, 445 moths were captured in raw material-baited traps and 1,566 moths in sex pheromone-baited traps. There were no clear differences in the attractiveness to the moths between different kinds of rice wines and fruit baits when the attractiveness of fifteen different rice wines and seven fermenting fruit baits were compared in the apple orchards. One rice wine (‘Chung-ju’ rice wine) and fermenting pineapple showed somewhat higher attractiveness to G. molesta than other raw baits, which will be re-examined in 2011 – 2012 periods.

In order to develop the substitutive materials for natural baits of swimming crab pots, the attracting effects of swimming crab such as the preference of baits which were made of the by-products of marine and stock raising through the water tank experiments and fishing experiments. On the investigation of mean entrapped catch number to the pot by the baits after putting the 4 kinds of baits, mackerel(M), mackerel with grinded mackerel s internals(MMI), mackerel with tuna s internalsMMI) and makerel with grinded krill(MK) each in one pot by turns, MMI and MK were entrapped mean 3.9(13.0%) and they were a little more comparing to M, and MTI is least with mean 2.1(7.0%)(F=12.913, P 〈 0.05). Otherwise, on the preference investigation of swimming crabs by the baits after putting the 4 kinds of baits in the 4 pots each, M was entrapped mean 3.0(10%), but MMI, MTI and MK were mean 1.2(4.0%), 1.0(3.3%) and 1.5(5.0%) each and they were only 30-50% of M(F=13.398, P 〈 0.05). On the preference investigation of swimming crabs by the 5 kinds of baits, mackerel(M), and krill(K), manila clam(MC), pig s fat(PF) and chicken s head(CH) which were used in substitutive baits, M was entrapped mean 3.2(10.7%), but K was about 50% of catch of M with mean 1.6(5.3%), and MC, PF and CH were very few with mean 0.1-0.2(0.3-0.7%)(F=89.186, P 〈 0.05). On the preference investigation of swimming crabs by the pots which were put each the 3 kinds of baits, original krill(K), grinded krill with gluten and soybean oil cake(KGGS) and grinded krill with gluten, soybean oil cake and glycine(KGGSGL) in the blue fluorescent hexahedral plastic bait cages(BF), and which were put the mackerel(M) in the non-fluorescent hexahedral red plastic bait cage(RFN), it was entrapped mean 3.0(10.0%) in the pot which was put the mackerel in the RFN, and the same level in the pots which were put the K and KGGSGL in the BF, but it was mean 2.0(6.7%) in the pots which was put the KGGS in BF and it was decreased by 30% of catch comparing to RFN(F=3.750, P 〈 0.05). On the preference investigation of swimming crab by the pots which was put grinded tuna with gluten, soybean oil cake and glycine(TIGSGL) in the blue fluorescent hexahedral plastic bait cage(BF), and which was put mackerel(M) in the nonfluorescent hexahedral red plastic bait cage(RFN), it was entrapped mean 3.3(11.0%) in the pot which was put mackerel in RFN, and mean 2.7(9.0%) in the pot which was put TIGSGL in BF and it was about 15% less comparing to use bait M(t=1.387, P 〈 0.05). As a results of fishing experiments, a plan for enhancing catching efficiency of TIGSGL will be required because catching efficiency of TIGSGL, alternative bait, was half of fish catching efficiency of natural bait using mackerel. Fishing experiments were conducted 3 times using reinforced substitutive artificial bait that is reinforced attractive effect of TIGSGL and composed of tuna intestine, grinded mackerel, gluten, soybean cake, glycine and alanine(TIMGGSGLA). Catching efficiency of TIMGGSGLA was about 80% of that of natural bait made of mackerel.

In order to develop the substitutive materials for natural baits of swimming crab pots, the fluorescent characteristics of the baits were analyzed, and the preference of fluorescent dyes were investigated by the mean entrapped catch number to the pots through the water tank experiments and fishing experiments. On the investigation of fluorescent characteristics by the 5 kinds of baits, mackerel, krill, manila clam, pig's fat and chicken's head which were used in substitutive baits for test in the UV long wave(365nm) area, it showed clear blue fluorescence in the skin of mackerel, shell of krill, manila clam and bill of chicken's head, and green fluorescence in the mackerel s muscle and internals, and yellow fluorescence in the pig's fat and chicken's head. On the investigation of fluorescent characteristics by the bait cages in the UV short wave(254nm) and long wave(365nm) area, it showed each green, red and blue fluorescence in the cylinderical or hexahedral red plastic bait cages which were painted each green, red and blue fluorescence dyes, but it showed yellowish green flourescence in the cylinderical or hexahedral red plastic bait cage which was painted yellow fluorescent dye. On the preference investigation of the fluorescent dyes of swimming crabs by the 5 kinds of the bait cages which were put the mackerel in the non-fluorescent red plastic cage(RFN), red, yellow, green and blue fluorescent plastic cages(RF, YF, GF, BF) each, nonfluorescent red plastic cage(RFN) was entrapped mean 2.0(6.7%), but blue fluorescent plastic cage(BF) was mean 5.0(16.7%) and it was more 2.5 times comparing to RFN, and red fluorescent cage(RF) was same level and green fluorescent cage(GF) was 50% of catch number comparing to RFN, and yellow fluorescent cage(YF) was entrapped nothing(F 46.324, P 〈 0.05). On the investigation of the entrapped catch number to the pots which were put the mackerel in the blue fluorescent hexahedral plastic cage(HP) and blue fluorescent silicon mackerel model cage(SM), HP was mean 3.4(11.3%) and it was a little more comparing to SM which was entrapped mean 3.2(10.7%)(t 0.775, P 〉 0.05). Fishing experiments on the preference investigation of swimming crabs by the pots which were put in the non-fluorescent red plastic cage(RFN) and blue fluorescent plastic cage(BF) were conducted 3 times. Mean catching number and weight of RFN were 71.7 ind.(18.3%) and 16.9kg(64.3%), and those of BF were 93.0 ind.(23.1%) and 19.8kg(64.5%), respectively.

In order to develop the artificial bait of trap for swimming crabs, the attracting effect was analyzed in the duration time of attracting effect, preference and shape recognition to the bait in the water tank and field experiments. According to the result which investigated the mean entrapped number of swimming crabs into the trap for each bait(mackerel, sardine and anchovy) in water tank experiments, the mean entrapped number was found to be higher in the trap with mackerel(6.9 ind.; 23.0%) than in other traps with sardine(5.2 ind.; 17.3%) and anchovy(3.4 ind.; 11.3%). In addition, the mean entrapped ratios of swimming crabs into the traps for 3 kinds of baits were estimated to be over 80% all in 5 hours after soaked in the water tank. For preference of swimming crabs to 3 kinds of baits, the trap with mackerel(6.9 ind.; 23.0%) was evaluated to be higher than the traps with sardine(2.8 ind.; 9.3%) and anchovy(0.2 ind.; 0.7%). In the field experiments, the mean entrapped number of swimming crabs into the trap with mackerel and sardine were found to be similar as 120 and 118 ind., but the trap with anchovy was only 85 ind.. According to the result which investigated the shape regnition of swimming crabs to the bait used mackerel, divided into 8 pieces, 16 pieces and grinded mass in water tank experiments, the mean entrapped number of swimming crabs into the traps that includes the mackerel divided into several pieces was significantly decreased. Moreover, by the presence of skin of mackerel for bait, there was a great difference in the entrapped number of swimming crabs. Therefore, it was thought that the swimming crab was attracted to the bait and entrapped into the trap would indicate that not only the chemical stimuli such as the attactant, but also the visual stimuli such as the shape recognition to the bait might be closely related.

The aim of this study was to develop the artificial trap baits being able to replace the natural anchovy or sardine baits. The fishing trials for evaluating the fishing efficiencies of the artificial baits were conducted with commercial traps at coastal areas near Tongyoung, southern Korea. The preferable artificial baits which were made of minced anchovy, sardine, mackerel, or fish surplus products mixed with wheat flour into dry or wet pelleted or crumbled types, were showed some merits as the following. \circled1 Fishing efficiencies of the artificial baits were same or little higher level than that of the natural bait anchovy; \circled2 bait cost were same or little lower level; \circled3 dry artificial baits stocked in the room condition showed good fishing efficiencies; \circled4 making of artificial baits were very simple; \circled5 there were no harm to the fish and little pollution to the environment; \circled6 the releasing rate of attractants in the artificial or natural bait showed similar patterns. The artificial baits using the fish by-products which were vacuum freeze-drying extract of boiled anchovy or tunas extract, showed lower catches in the conger eel, Coner miriaster, but similar catches in the crabs, mainly Charybdis japonica, to the those of natural anchovy bait.

5.0% micro-capsuled fenitrothion, 2.0% hydramethylnon, 또는 0.8% chlorpyrifos가 주요 활성 물질인 독먹이 제제의 방제효과를 알아보기 위하여 바퀴, Bfattella germanica (L)가 번식하고 있는 15개의 식당에서 8주동안 야외실험을 실시한 결과는 다음과 같다. 바퀴 성충에 대한 방제효과는 mIcro-capsuled fenitrothion 제제 (평균 82.8%)가 chlorpyrifos 제제 (평균 68.4%)보다 유의성 있게 높았으나 hydramethylnon 제제 (평균 86.1%)와는 거의 같은 효과를 보였다. 또한, 바퀴 자충에 대한 방제효과는 3가지 독먹이 제제가 거의 같은 유효성(평균 74.9- 82.8%)을 나타내였다. 독먹이 소모량을 조사한 결과, 8주 동안에 서식 바퀴에 의한 micro-capsuled fenitrothion 제제의 소모량은 0.11 g으로, 평균 90% 이상 남아 있었으나, hydramethylnon 제제는 바퀴에 의한 소비량(O.72g)이 현격하게 많아서 잔여량이 평균 57%로 나타났다. 이러한 결과는 hydramethylnon의 살충 기작의 특성, 유인먹이의 재료, 서식 바퀴의 밀도와 관계가 있는 것으로 추정되었다.

통발어구의 미끼를 개선하기 위한 기초실험으로 16종의 천연미끼와 아미노산 및 핵산 관련물질을 사용하여 현장에서 어획실험을 행한 결과는 다음과 같다. 1. 주된 어획 생물은 붕장어, 왜볼락, 망둑, 게, 골뱅이 및 불가사리 등이었다. 2. 어획 생물별로 유인성활이 강한 천연미끼는 아래와 같다. 붕장어: 멸치, 정어리, 참갯지렁이. 망둑: 멸치, 정어리, 참갯지렁이, 명태, 크릴. 왜볼락: 줄새우, 갯가재. 게: 꽃새우, 크릴, 오징어육, 멸치, 명태, 바위갯지렁이. 골뱅이: 멸치, 정어리, 털게, 꽃새우, 개조개. 불가사리: 정어리, 우럭조개, 개조개, 오징어육. 3. 아미노산과 핵산관련물질은 천연미끼보다 유인활성이 낮았지만 비교적 어획을 많이 보인 물질은 다음과 같다. 붕장어: Taurine, L-Glu, L-Gly, L-Met, IMP. 망둑: L-Glu, L-Met. 왜볼락: L-Met. 게: Taurine. Hx. 골뱅이: L-Gly, L-Met. 불가사리: IMP.

현장실험을 통한 붕장어 생미끼의 유인활성을 검토하기 위하여 상용의 PVC통발 및 스프링식 그물통발을 이용하여 충무근해, 사량도 부근해역 및 남해도 미조항 부근에서 행한 실험 결과를 요약하면 다음과 같다. 1. 어획 적정 미끼중량을 파악하기 위한 실험의 결과정어리의 경우 50g이상에서는 어획율 사이에 뚜렷한 차이가 없어 최소 어획 적정 미끼중량이 50g으로 판단되었다. 2. 어류, 두족류, 갑각류, 패류 및 환형동물등 24종의 시료중 정어리와 멸치 및 참갯지렁이의 유인활성이 가장 강하였고 명태, 오징어 간유, 홍합 및 우렁쉥이 껍질의 유인활성이 다음으로 강하였으며 다른 시료에서는 거의 유인활성을 보이지 않았다. 3. 정어리의 부위별 유인활성은 육에서 가장 강하게 나타났으나, 머리 및 내장에서는 유인황성이 약했다. 또한 멸치와 정어리의 자숙액은 생미끼보다 유인활성이 조금 약하였으나 부패시킨 미끼에서는 유인활성이 아주 약하였다.

인공추출물을 조제하여 유인활성을 검토하였으며 9종의 아미노산과 2종의 핵산 관련물질을 표준품으로 현장에서 omission test를 실시한 결과는 다음과 같다. 1. 생멸치 및 냉동정어리의 에탄올 추출물과 잔사의 유인활성을 검토한 결과, 에탄올 추출물에서 강한 유인활성을 나타내었다. 2. 생멸치의 구성성분과 같은 농도로 인공합성품을 조제하여 현장실험을 한 결과, 유인활성이 생시료의 에탄올 추출액보다 약했으나 아미노산 합성물질과 핵산 관련 물질이 다른 물질들보다 유인활성이 강한 것으로 나타났다. 3. 9종의 아미노산에 대하여 omission test를 실시한 결과 L-글루타민산, L-글리신, 타우린 및 L-메티오닌은 붕장어에 대하여 유인활성이 강한 것으로 나타났으며, L-라이신이나 DL-폐닐알라닌은 기피활성이 있는 것으로 나타났다. 4. 2종의 핵산 관련물질중 IMP는 유인활성이 있었으나 하이포산틴은 없었다.

원통 및 직육면체 등의 단순한 모형함정과 모형통발, 재료의 종류와 입구의 형태, 입국의 경사각도에 따른 입롱과 도피행동 및 미끼의 냄새에 유집되는 행동 등에 대한 태평줄생우의 선택행동을 수조에서 관찰하여 비교한 결과는 다음과 같다. 1. 망지재료가 P. A, P. E 및 면인 경우 새우의 입롱수는 차이가 거의 없었으며, 삼각형, 사각형의 입구단면보다는 원형에 더 많이 들어 갔고 입구의 경사가 클수록 들어가는 미수가 적었다. 2. 모형함정의 입구경사가 0˚, 90˚일 때부다 30˚, 60˚일 때가 시간의 경과에 따른 새우의 도피율이 낮았으며, 직육면체의 평면입구일 때보다는 원통의 원추형 입구일 때 도피율이 적었다. 3. 미끼의 축출용액에 모여드는 새우의 수는 전갱이와 바지락, 새우와 고등어, 고등어육과 내장 등 간에는 차이가 거의 없으나 고등어와 멸치젓, 족발과 새우간에는 각각 고등어와 새우 육에 더 많이 모여들었다. 4. 먹이가 들어 있는 모형통발에는 없을 때의 3배 정도 입롱수가 많았고, 미끼가 없을 경우 입구의 수가 1개와 2개일 때는 입롱수에 차이가 거의 없으나 도피수는 2개일때가 훨씬 많았다.