In order to investigate the effect of land reclamation on the life of local fishermen, a survey was conducted with the 246 people living in 12 different adjacent villages located in Hwasung District. It was found that income of residents decreased, while the amount of debts increased after reclamation. 65% of the people surveyed answered that their economic situation became worse than before and they blamed the decrease of fishery resources as the main cause. It further led to the loss of the family’s means of livelihood. 40% of the people surveyed claimed that they wanted to leave the fishing village and believed that reclamation divested them of all their hope for the future. Regarding to the effect of reclamation project on the image of Hwasung District, most of the respondents have a negative view on the reclamation project. They have negative views even on the tourism, regional welfare, employment of community residents and local infrastructure. Therefore, it can be concluded that local community residents thought that reclamation project had negatively influenced their overall quality of life.

Recently, pollution problem in coastal water has become more serious and pollution including red tide serves as a main reason for reduction of fishes resources. Particularly, nutrients such as nitrogen and phosphorus are the most serious pollutants. Normally, biological wastewater treatment process is used in removing such nutrients. However, it is difficult to adopt the biological wastewater treatment process to a small-scale fish processing factory in case of using seawater as wash water. Thus, removing nutrients through struvite crystallization is investigated in this study for treating shrimp processing wastewater. Experiments were conducted by varying molar ratio of Mg2+:NH4-N:PO4-P from 1:1:1 to 2:1:1. It can be concluded that optimum molar ratio is 1:1:1. Struvite crystallization process is compared with chemical coagulation process using PAC and struvite crystallization process is proven as the more effective process in removing nutrients from wastewater. In view of results obtained from these experiments, struvite crystallization process is a promising method in removing nitrogen and phosphorus from wastewater; however, not so good in removing organics. Thus, struvite crystallization process is suitable as the pre-treatment process in treating shrimp processing wastewater and additional biological process is needed to remove organics.

Population densities of aerobic heterotrophic bacteria by plate counting method were in the range of 3.5 ± 0.2 × 106~1.8 ± 0.7 × 107 cfu g−1 dry wt. from the grasses of Gunsan Country Club which sampled from August to October, 2011. We selected the celluloytic bacteria from aerobic heterotrophic bacteria and identified 6 strains among ninety-six isolates by using 16S rDNA analysis. As a result, four isolates belonged to Firmicute group, two isolates belonged to Gamma-proteobacteria group and Actinobacteria group, respectively. We also analysed the cabon sources utilization patterns of six isolates. D-cellobiose, D-Fructose, D-Galactose, D-Mannose, DMelibiose, D-Psicose, Gentiobiose, Lactulose, L-Arabinose, L-Fucose, Turanose, α-D-Glucose, and α-D-lactose among 95 carbon sources are utilized by all six isolates.

In order to develop the substitutive materials for natural baits of swimming crab pots, the attracting effects of swimming crab such as the preference of baits which were made of the by-products of marine and stock raising through the water tank experiments and fishing experiments. On the investigation of mean entrapped catch number to the pot by the baits after putting the 4 kinds of baits, mackerel(M), mackerel with grinded mackerel s internals(MMI), mackerel with tuna s internalsMMI) and makerel with grinded krill(MK) each in one pot by turns, MMI and MK were entrapped mean 3.9(13.0%) and they were a little more comparing to M, and MTI is least with mean 2.1(7.0%)(F=12.913, P 〈 0.05). Otherwise, on the preference investigation of swimming crabs by the baits after putting the 4 kinds of baits in the 4 pots each, M was entrapped mean 3.0(10%), but MMI, MTI and MK were mean 1.2(4.0%), 1.0(3.3%) and 1.5(5.0%) each and they were only 30-50% of M(F=13.398, P 〈 0.05). On the preference investigation of swimming crabs by the 5 kinds of baits, mackerel(M), and krill(K), manila clam(MC), pig s fat(PF) and chicken s head(CH) which were used in substitutive baits, M was entrapped mean 3.2(10.7%), but K was about 50% of catch of M with mean 1.6(5.3%), and MC, PF and CH were very few with mean 0.1-0.2(0.3-0.7%)(F=89.186, P 〈 0.05). On the preference investigation of swimming crabs by the pots which were put each the 3 kinds of baits, original krill(K), grinded krill with gluten and soybean oil cake(KGGS) and grinded krill with gluten, soybean oil cake and glycine(KGGSGL) in the blue fluorescent hexahedral plastic bait cages(BF), and which were put the mackerel(M) in the non-fluorescent hexahedral red plastic bait cage(RFN), it was entrapped mean 3.0(10.0%) in the pot which was put the mackerel in the RFN, and the same level in the pots which were put the K and KGGSGL in the BF, but it was mean 2.0(6.7%) in the pots which was put the KGGS in BF and it was decreased by 30% of catch comparing to RFN(F=3.750, P 〈 0.05). On the preference investigation of swimming crab by the pots which was put grinded tuna with gluten, soybean oil cake and glycine(TIGSGL) in the blue fluorescent hexahedral plastic bait cage(BF), and which was put mackerel(M) in the nonfluorescent hexahedral red plastic bait cage(RFN), it was entrapped mean 3.3(11.0%) in the pot which was put mackerel in RFN, and mean 2.7(9.0%) in the pot which was put TIGSGL in BF and it was about 15% less comparing to use bait M(t=1.387, P 〈 0.05). As a results of fishing experiments, a plan for enhancing catching efficiency of TIGSGL will be required because catching efficiency of TIGSGL, alternative bait, was half of fish catching efficiency of natural bait using mackerel. Fishing experiments were conducted 3 times using reinforced substitutive artificial bait that is reinforced attractive effect of TIGSGL and composed of tuna intestine, grinded mackerel, gluten, soybean cake, glycine and alanine(TIMGGSGLA). Catching efficiency of TIMGGSGLA was about 80% of that of natural bait made of mackerel.

In order to develop the substitutive materials for natural baits of swimming crab pots, the fluorescent characteristics of the baits were analyzed, and the preference of fluorescent dyes were investigated by the mean entrapped catch number to the pots through the water tank experiments and fishing experiments. On the investigation of fluorescent characteristics by the 5 kinds of baits, mackerel, krill, manila clam, pig's fat and chicken's head which were used in substitutive baits for test in the UV long wave(365nm) area, it showed clear blue fluorescence in the skin of mackerel, shell of krill, manila clam and bill of chicken's head, and green fluorescence in the mackerel s muscle and internals, and yellow fluorescence in the pig's fat and chicken's head. On the investigation of fluorescent characteristics by the bait cages in the UV short wave(254nm) and long wave(365nm) area, it showed each green, red and blue fluorescence in the cylinderical or hexahedral red plastic bait cages which were painted each green, red and blue fluorescence dyes, but it showed yellowish green flourescence in the cylinderical or hexahedral red plastic bait cage which was painted yellow fluorescent dye. On the preference investigation of the fluorescent dyes of swimming crabs by the 5 kinds of the bait cages which were put the mackerel in the non-fluorescent red plastic cage(RFN), red, yellow, green and blue fluorescent plastic cages(RF, YF, GF, BF) each, nonfluorescent red plastic cage(RFN) was entrapped mean 2.0(6.7%), but blue fluorescent plastic cage(BF) was mean 5.0(16.7%) and it was more 2.5 times comparing to RFN, and red fluorescent cage(RF) was same level and green fluorescent cage(GF) was 50% of catch number comparing to RFN, and yellow fluorescent cage(YF) was entrapped nothing(F 46.324, P 〈 0.05). On the investigation of the entrapped catch number to the pots which were put the mackerel in the blue fluorescent hexahedral plastic cage(HP) and blue fluorescent silicon mackerel model cage(SM), HP was mean 3.4(11.3%) and it was a little more comparing to SM which was entrapped mean 3.2(10.7%)(t 0.775, P 〉 0.05). Fishing experiments on the preference investigation of swimming crabs by the pots which were put in the non-fluorescent red plastic cage(RFN) and blue fluorescent plastic cage(BF) were conducted 3 times. Mean catching number and weight of RFN were 71.7 ind.(18.3%) and 16.9kg(64.3%), and those of BF were 93.0 ind.(23.1%) and 19.8kg(64.5%), respectively.

In order to evaluate the biodegradability of bait used in the pot for swimming crab, water tank experiments were conducted. Mackerel is the most commonly used natural form of bait to catch the swimming crabs, and therefore was used in this experiment for the biodegradability according to the manufacturing process of the bait. From the biodegradability test on chemical oxygen demand(COD), total nitrogen(T - N), total phosphorus(T - P), ammoniac nitrogen, nitrite nitrogen and nitrate nitrogen per unit weight of the bait based on the production rate and the accumulated amount of pollutants, it was concluded that the smaller the size of the mackerel pieces, the higher the production rate and accumulated amount of organic matter and nutrients which was unfavorable to water pollution. The amount of pollutants released from the intestine of the tuna was similar with that from the whole mackerel. For the operation period of 111 days, the accumulated concentrations of tested pollutants from the tuna which were 67.3 mgCOD/g d, 86.4 mgT N/g d, 3.1 mgT - P/g d, were almost half comparing with those from the mackerel which were 65.7 - 94.4 mgCOD/g d, 83.8 - 109.4 mgT - N/g d, 3.1 - 5.2 mgT - P/g d. The amount of pollutants released from the intestine of the tuna was slightly less than that from the mackerel that was cut into 8 pieces. but more than that from the mackerel which was not cut into pieces. Therefore, it can be concluded that the key factor in determining water pollution potential is not the kind of bait, but the processing or preparation method used.

Analytical method using capillary GC/ECD was developed to determine trace residues of chlorfluazuron, 1-[3, 5-dichloro-5-trifluoromethyl-2-pyridyloxy)phenyl)-3-(2, 6-difluorobenwyl), in meat, and applied to analyze the residues in domestic and imported meats. The analytical scheme developed does not require column chromatographic cleanup; chlorfluazuron was extracted with diethyl ether and petroleum ether (50: 50), partitioned against acetonitrile, cleaned up with silica Sep-Pak cartridge, identified GC/ECD, and comfirmed by GC/MS. The mean recoveries of the pesticide in meat fortified with standard solution 0.1, 0.5, 1.0 mg/kg were ranged from 82 to 95%. The limit of detection and limit of quantitation were 0.001 and 0.005 mg/kg, respectively. Chlorfluazuron residues were not found in domestic samples, but found in imported Australian beef ranging from 0.02 to 0.17 mg/kg, detected by 18% among the samples.