본 연구는 마치종 황색 알곡 품종으로 등록된 강일옥 옥수수 알곡의 처리별 시료 및 속대의 일반성분, 생리활성 검정, 색소 함량을 분석하여 기초자료로 제공하고자 수행되었다. 강일옥 옥수수를 알곡, 거피된 알곡, 알곡 껍질 및 속대로 분리하여 일반성분 분석 결과, 수분함량은 거피된 알곡이 12.04%로 가장 높았고 알곡 껍질의 조회분, 조지방, 조단백질 함량은 각각 3.19%, 8.59%, 12.01%로 강일옥 옥수수 처리별 시료 중 가장 높은 함량을 나타내었다. 강일옥 옥수수 처리별 시료의 무기성분 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 철, 망간 및 인의 6종이 검출되었으며 공통적으로 검출된 주요 무기성분은 칼륨과 인이었다. 강일옥 옥수수 속대의 칼륨 함량이 114.33 mg/ 100 g으로 가장 높았으며 알곡 껍질의 인 함량이 2412.40 mg/100 g으로 가장 높게 측정되었다. 강일옥 옥수수 처리 별 시료의 항산화 활성 검정 결과, 강일옥 옥수수 속대 추 출물의 DPPH radical 소거능은 93.05%, ABTS radical 소 거능은 79.88%로 처리별 시료 중 가장 높게 측정되었으며 알곡, 거피된 알곡 및 알곡껍질 추출물의 DPPH 소거 능은 모두 70% 이상으로 우수한 항산화활성을 나타내었다. 강일옥 옥수수 처리별 시료의 항염 활성 검정 결과, 강일옥 옥수수 처리별 시료 추출물은 Raw264.7 대식세포 의 생존율에 영향을 미치지 않은 것으로 나타났으며 NO 생성량은 모든 시료 처리군에서 LPS 단독 처리군(con(+)) 에 비하여 유의적으로 NO 생성을 억제 하는 것으로 나타났다. 강일옥 옥수수 처리별 건조분말시료의 카로티노이드 색소 함량을 분석한 결과, 모든 시료에서 β-carotene은 검출되지 않았고, lutein과 zeaxantin은 알곡과 알곡 껍질 에서 검출되었으며 거피된 알곡과 속대에서는 검출되지 않았다. 강일옥 옥수수 알곡의 lutein과 zeaxantin의 함량은 각각 115.30 μg/100 g, 50.92 μg/100 g이었으며 알곡껍질의 lutein과 zeaxantin의 함량은 각각 37.98 μg/100 g, 19.27 μg/100 g이었다.

This study was performed to investigate the content of carotenoid (α-carotene, β-carotene, β-cryptoxanthin, capxanthin, lutein, lycopene, zeaxanthin) and physicochemical characteristics (size, color, dietary fiber, total tannin content) of unripen and ripen persimmons (Diospyros kaki) by seven cultivars. The length of the unripen and ripen persimmons ranged from 40.87-64.75 and 48.80-80.29 cm, the width were from 44.03 to 66.73 and 57.65 to 91.53 cm, respectively. Ripen persimmons were showed in the highest Hunter’s L, a and b values. Total tannin content were resulted of unripen- and ripen persimmons ranged from 7.58 to 19.80 and 2.36 to 5.85 mg/g. Total carotenoid content were from 1.09 to 6.45 and 7.46 to 29.46 μg/g, respectively. From the above results, unripen- and ripen persimmon fruit could be useful for the health functional food.

Chinese cabbage grown during autumn season is confronted with drought conditions for a certain period, especially during the early growth stage. In this study, we investigated the effects of drought stress on plant growth characteristics, as well as free amino acid, carotenoid, and proline in Chinese cabbage. Chinese cabbage seeds (Bulam Plus) were germinated, and all the seedlings were transplanted into plastic containers (28 ㎝ diameter × 22 ㎝ high) containing a commercial growth medium. The soil water content was measured and maintained at 10% for the drought-stressed plants and at 30% for the control plants, for three weeks. The results revealed that plant growth parameters were lower in the drought-stressed plants than in the control plants. The total free amino acid content tended to decrease in both drought-stressed and control plants with time. The total free amino acid content was found to be lower in the drought-stressed plants than in the control plants and the proline content was unaffected. Moreover, at three weeks after treatment, carotenoid content in drought stressed plants was significantly higher than that in the untreated plants. We believe that our study makes a significant contribution to the literature because the effects of drought stress on plant growth parameters, free amino acid, carotenoids, and proline accumulation in autumn growing cultivar of Chinese cabbage have not been widely studied in Korea, and our study provides valuable information in this regard, as Chinese cabbage is consumed throughout the year in Korea.

Chinese cabbage (Brassica rapa ssp. pekinensis) is one of the most important vegetables and widely cultivated in Asia countries including Korea and China. Recently, whole genome sequence and full-length cDNA information of this species became available, which are encouraging genetic studies of this species to characterize agricultural important traits. Orange-colored (Or) cultivar of Chinese cabbage has inner leaves in orange, whereas other cultivars generally cultivated have yellow (Ye)- or white-colored inner leaves. In this study, we investigated phenotypes and carotenoid biosynthesis genes related to color variation in the Or cultivar. Firstly we compared the carotenoid content and composition between the Or and Ye cultivars by HPLC analysis. The inner leaves of Or cultivar contained approximately 9-fold high β-carotene content, whereas content of both lutein and violaxanthin was decreased to less than 30%, compared to Ye cultivar. Or cultivar was segregated with ratio of 3:1 in F2 population derived from crossing between Or and Ye inbred lines, indicating that Or phenotype is controlled by single recessive gene. To identify this gene, we investigated the expression of several genes involved in carotenoid biosynthesis by RT-PCR analysis. Among genes tested, two encoding putative carotenoid isomerase (CRTISO) and phytoene desaturase (PDS) were identified to show different expression between Or and Ye cultivars. Through further analysis of genomic DNA regions of these two genes, we could expect that several mutations such as InDel and base-substitution occurred and then affected expression of these genes in Or cultivar. In this presentation, I will introduce more detailed results for Or cultivars.