Background : The roots of Codonopsis pilosula (Franch.) Nannf are used as medicines such as expectorants, and they are edible. Codonopsis pilosula (Franch.) Nannf currently has a very small cultivation area, and there are no breeding cultivars, most of the native species are cultivated in the farm house. Therefore, it is important that identification of collecting species characteristic in order to breeding strategy. In this study, morphological features of the leaves and stems of the collecting species in the area were observed. Methods and Results : The morphological characteristics of mansam collected from three areas including Cheolwon (41 accession), Jeongseon (19 accession), and Pyeongchang (15 accession) were investigated at the flowering stage on leaf and stem. As a result of investigation of leaf characteristics, The leaf shape was the most frequent with 78.9% in the kidney type, 8.5% in the cardiac type, 7.0% in the wide-elliptical type, and 2.8% in the egg and egg-oval type. The leaf length was the most frequent with 67.1% in case of short, 28.6% in case of middle, and 4.3% in case of long. The leaf width was 67.1% in case of middle, 30.0% in case of broad, and 2.9% in case of narrow. Leaf hair was 47.1% in case of plenty, 27.1% in case of middle, and 25.7% in case of small number. The degree of greenness of the leaves was 60.0% in case of middle, 31.4% in case of darkness, and 8.6% in case of lightness. The degree of pigmentation of anthocyanin in petiole was 41.4% in case of lightness, 31.4% in case of darkness, and 27.1% in case of middle. As a result of investigating the characteristics of stem, The presence and absence of hair on the stem was 95.7% in case of little, 2.9% in the middle, and 1.4% in case of plenty. The degree of staining of anthocyanin in the stem was 42.9% in case of middle, 30.0% in case of darkness, and 27.1% in case of lightness. Conclusion : We intend to use this data as a basic data for cultivating varieties by supplementing investigation of traits such as expansion of collection area and investigation object and flower shape in the future.

환경조건이 불량한 산간고냉지에서 주로 재배되는 만삼은 종자의 구조적 결함으로 인한 발아율 저조로 입묘가 불량하게 됨으로서 재배에 실패하는 경우가 빈번하다. 따라서 파종전 종자처리를 통하여 만삼의 입묘율을 향상시키고자 종자의 발아와 관련이 있는 파종 후의 광조건을 고정한 후 priming, GA3, 건조, 침종 등 파종전 종자에 손쉽게 가할 수 있는 처리의 효과를 구명하고 이들 처리를 모형화하기 위하여 본 연구를 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 발아율은 0.1 mM에 3일간 1일 12시간의 청색광 또는 적색광을 가하면서 GA3를 처리하는 것보다는 적색광 또는 암상태에서 CA(NO3)2 150 mM에 2일간 priming 처리하는 것에서 높았다. 2. Priming중에 처리용기를 교반시키거나, priming 후에 행하여지는 세척이 발아율을 향상시키는 것으로 조사되었다. 3. Ca(NO3)2 150 mM에 2일간 priming처리된 만삼종자의 최적건조 방법은 빛이 없는 암상태에서 35℃에 4시간 건조하는 것이 가장 양호한 발아율을 보였다. 4. Priming 후에 건조처리된 종자는 파종 직전 2일간 침종하여야만 발아율과 유묘출현율이 가장 높은 것으로 나타났다. 5. 이상의 결과로부터 만삼의 입묘율을 높이기 위한 파종전 종자처리는 20℃에서 1일 12시간의 적색광을 가하면서 Ca(NO3)2 150 mM에 2일간 처리용기를 흔드는 방법으로 priming된 종자를 물에 세척한 후 35℃에서 4시간 적색광을 처리하여 건조한 다음 파종 직전 20℃의 물에 2일간 침종하는 과정으로 요약된다.

Codonopsis lanceolata and C.pilosula medicinal plants were subjected to preliminary antitumor screening test with Sarcoma 180 ascites and screning on V-79 cell. This ex-periments Iwere conducted in accordance with the Total packed cell Volume methodand Cytotoxicity method .