Sensory evaluation of shucking pressure, pressure holding time, seeding method, difference in full shucking rate in the aquaculture area and shucking oyster was performed using an ultra-high pressure oyster shucking machine. The reaching time for each target pressure is 2.2-2.4 MPa/sec in the range of 180 MPa to 240 MPa. had a rate of pressure rise. There was a difference of 0.5-1.7℃ in the range of 24-27℃ in the seawater temperature before and after the pressure treatment inside the pressure vessel, but there was no specific increase or decrease in seawater temperature. When only the shucking pressure is increased without the pressure holding time, the critical shucking pressure at which the oyster shell is opened and the flesh is peeled in the range of 200 to 220 MPa. When the critical shucking pressure is reached, the oyster sample in the closed vessel is expected to be shucked by about 40%. If there is no pressure holding time when judged only by full shucking, an increase in pressure of about 1.5 MPa is required to further shuck 3% of the oyster population. The oyster samples cultivated in the south coast of Korea were subject to full shucking under the conditions of 220 MPa shucking pressure and two minutes (120 seconds) of pressure holding time, and the difference in the pressure of the oysters according to the oyster seeding method and the farming area was minute. Finally, the condition of 220 MPa of shucking pressure and three minutes of pressure holding time was the best at 1.52 when the result of the sensory evaluation performed manually was set to 1.0. Next was 1.4 under the conditions of 220 MPa of shucking pressure and one minute of pressure holding time (60 seconds), and 1.3 under the condition of 220 MPa and two minutes of pressure holding time (120 seconds). Therefore, it is considered that the most desirable shucking conditions, considering the efficiency and sensory evaluation results, are the conditions of 220 MPa shucking pressure and two to three minutes of pressure holding time.

By applying super-high pressure (150-250 MPa) to a sealed pressure vessel, it is possible to make oyster shucking machine that automatically opens two-sheet shellfish or oysters. Possibility of developing a shucking machine was confirmed by identifying the working pressure for meat of oysters produced in the southern coast and conducting sensory evaluation of meat oysters. As a result of confirming the shucked oysters under super-high pressure of 150 MPa in the pressure vessel, the number of type A with separated shells and well-separated meat was 22 and type B with both shells and internal meat and shells not separated. For the oysters that were treated at 175 MPa, there were 58 type As with shell separated and meat well separated and 42 type Bs without oyster shells and insides. When looking at the oysters shucked at 200 MPa in the pressure vessel, the number of type A was 86 and type B was 14 accounting for 86% of oysters with good marketability. As a result of shucking oysters by applying 250 MPa, 96% type A oysters and 4% type B oysters were obtained from the total specimen. The total specimen oyster weight used in the conducted experiment was 6 kg, the average oyster shell weight was 3.99 kg and the average oyster meat weight was 1.25 kg. Therefore, the fatness of oyster meat, which measures the added value of oysters, is 20.8%. Sensory evaluation was conducted on thinned oysters by hand and type A oysters shelled by machine with an operating pressure of 200 MPa. The hand-worked oyster sample scored 4.7 points only in salty taste, and scored 5.0 or higher in color, shape, smell, fishy taste, texture and preference.

본 연구에서는 2016년 6월부터 12월까지 통영 한산도 및 거제 동부 굴 양식장 밀집해역의 환경요인의 변동에 따른 식물플랑크톤 군집변화의 계절특성을 파악하고자 하였다. 조사기간 동안 수온은 14~28.8°C로, 염분은 29.4~34.2 psu의 범위로 변화하였다. 질산염+아질산염의 농도는 6~7월 동안 표층에서 3.0 μM 전후로, 8월과 9월초까지 제한농도 이하로, 그 후 9월말부터 점차적으로 증가하는 양상을 보였다. 암모니아는 전반적으로 낮게 나타나 계절적 특성이 뚜렷하지 않았다. 인산염은 표층에서 0.01~0.7 μM의 범위로 보였고, 질산염+아질산염과 유사한 계절적 변화를 보였다. 규산염 평균농도는 표층에서 10.7 μM과 저층에서 15.7 μM로, 전 계절에 있어 식물플랑크톤의 성장에 제한인자로 작용하지 않았다. 식물플랑크톤 군집조성은 규조류, 와편모조류, 은편모조류의 비율은 각각 61.2%, 22.5%, 13.6%로 관찰되었다. 6월 말, 외측해역 (T1정점)을 중심으로 와편모조류 Prorocentrum donghaiense가 우점하였고, 이후 7월 Cryptomonas spp.와 규조류 Chaetoceros spp.가 상대적으로 높게 점유하였다. 태풍 이후 성층이 붕괴된 9월말에서 10월까지 규조류 Pseudonitzschia spp.와 Chaetoceros spp.가 전 정점에서 우점하였으며, 12월에는 와편모조류 A. sanguinea가 최대 1.7×105 cells L-1로 적조주의보 수준으로 높게 관찰되었다. 결과적으로 거제도 한산도주변해역에서는 계절적 성층의 형성과 소멸에 따른 식물플랑크톤 군집조성의 차이가 나타났고, 환경요인의 변화는 일차생산자인 식물플랑크톤 생물량 (biomass) 변동에도 영향을 미칠 수 있으며, 이는 양식굴산업의 생산에도 직결될 것으로 판단된다.

The purpose of this study was to investigate the seasonal distribution of phytoplankton as prey for oysters and to characterize the environmental factors controlling their abundance from June 2016 to May 2017, in the northeast coast between Tongyeong and Saryang Island, particularly for the oyster farming area. During the survey period, water temperature changed from 7.54°C in February to 29.5°C in August. The abnormal high temperature persisted during one month in August. Salinity was low due to summer rainfall and typhoon. The lowest level was 30.68 psu in September, and it peaked at 34.24 psu in May. The dissolved oxygen (DO) concentration ranged from 6.0-9.45 mg L-1, and the DO concentration in the surface layer was like that in the bottom layers. The seasonal trends of pH were also like those of DO. The pH ranged from 7.91 to 8.50. Nitrate with nitrite, phosphate, and silicate concentrations ranged from 0.14 μM to 7.66 μM, from 0.01 μM to 4.16 μM, and from 0.27 μM to 20.33 μM, respectively. The concentration of chlorophyll a (Chl. a) ranged from 0.37 μg L-1 to 2.44 μg L-1 in the surface layer. The annual average concentration was 1.26 μg L-1. The annual mean phytoplankton community comprised Bacillariophyta (69%), Dinophyta (17%), and Cryptophyta (10%), respectively. Dinoflagellate Prorocentrum donghaiense in June was the most dominant at 90%. In the summer, diatom Chaetoceros decipiens, Rhizosolenia setigera and Pseudo-nitzschia delicatissima were dominant. These species shifted to diatom Chaetoceros spp. and Crytophyta species in autumn. In the winter, high densities of Skeletonema spp. and Eucampia zodiacus were maintained. Therefore, the researchers thought that the annual mean Chl. a concentration was relatively lower to sustain oyster feeding, implying that the prey organism (i.e., phytoplankton) was greatly controlled by continuous filter feeding behavior of oyster in the vicinity area of the oyster culture farm.