The leaves of Hippophae rhamnoides were extracted with methanol and then further fractioned with hexane, ethyl acetate, butanol and water in order to investigate biological activity. Two flavonol glycosides were isolated and identified from ethyl acetate soluble fraction, which showed the strongest antioxidant activity (RC50 = 4.33 μg/ml). Isolated two compounds have shown strong free radical scavenging activity. Especially, quercetin 3-O-glucoside (RC50 = 2.60 μg/ml) was more active than α-tocopherol (RC50 = 4.67 μg/ml). Total phenol and flavonoid contents were the highest to 4.17 and 1.14 mg/ml in the ethyl acetate soluble fraction. In addition, ethyl acetate soluble fraction was shown to the strongest α-glucosidase inhibitory activity (IC50 = 137.88 μg/ml) among the fractions. The results suggest that leaves of H. rhamnoides could be a potential source of natural antioxidant.

Night-break, 일장조절, 월동가온 실험으로부터 얻어진 바위솔에 포함된 flavonol glycoside, kaempferol-3-O-β-D-glucoside(1), kaempferol-3-O-β-D-glactoside (2), kaempferol-3-O-β-D-rhamnoside (3), quercetin-3-O-β-D-glucoside (4) and quercetin-3-O-α-D-rhamnoside (5)의 함량이 분석되었으며 자연산 바위솔의 함량과 비교되었다. 월동가온 시험에서 얻은 flavonol glycoside 1-5의 함량은 자연산보다 감소하였으며 일장조절 실험 및 night-break 시험에서 얻은 flavonol glycoside 함량은 조사한 빛의 양이 증가함에 따라 증가하였다. 월동가온은 flavonol glycoside의 생성에 부정적으로 작용하는 반면 조사되는 빛의 양의 증가와 같은 경작조건은 flayonol glycoside의 생성에 유리하다.