The box tree pyralid, Glyphodes perspectalis, was reared on an artificial diet based on dried powder of box tree leaves, Buxus microphylla var. koreana, and INSECTA F-II (Nihon Nosan Co., Ltd) at a ratio of 3:7 (w:w) and analyzed in their developmental characteristics from 1st to 4th generation. The developmental period of larva reared on artificial diet was longer than that of larva reared on host plant. Significant difference in larval period was also observed between 1st and other generation groups. Pupation rate was the highest in 4th generation followed by 3rd, 2nd, 1st and host plant group. Significant difference in pupal period was noted between 1st and 3rd generation group. Emergence rate was the highest in 4th generation group followed by 3rd, host plant, 1st and 2nd generation group. There is a significant difference in male and female size between 1st and 4th generation group. Our result indicated that stable rearing of box tree pyralid through the year is possible by artificial diet, and the best adaptation to artificial diet was achieved at 4th generation.

The box tree pyralid, Glyphodes perspectalis, was reared on an artificial diet based on dried powder of box tree leaves, Buxus microphylla var. koreana, and INSECTA F-II (Nihon Nosan Co., Ltd) at a ratio of 3:7 (w:w) and analyzed in their developmental characteristics from 1st to 4th generation. The developmental period of larva reared on artificial diet was longer than that of larva reared on host plant. Significant difference in larval period was also observed between 1st and other generation groups. Pupation rate was the highest in 4th generation followed by 3rd, 2nd, 1st and host plant group. Significant difference in pupal period was noted between 1st and 3rd generation group. Emergence rate was the highest in 4th generation group followed by 3rd, host plant, 1st and 2nd generation group. There is a significant difference in male and female size between 1st and 4th generation group. Our result indicated that stable rearing of box tree pyralid through the year is possible by artificial diet, and the best adaptation to artificial diet was achieved at 4th generation. The pheromone components of this moth have been identified as (Z)-11-hexadecenal (Z11-16:Ald), (E)-11-hexadecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z-11-16:OH). We did several field tests, and found that there was a difference between Korea and Japan population.

In this study, we reared box tree pyralid, Glyphodes perspectalis, with artificial diet and identified sex pheromone. Insect F-II and leaf of box tree were used to make artificial diet. Box tree pyralid, Glyphodes perspectalis, is the most serious pest of box tree in Korea, and was recently introduced into Europe. The pheromone components of this moth have been identified as (Z)-11-hexadecenal (Z11-16:Ald), (E)-11-hexadecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z-11- 16:OH) in Japan. In this study, we identified pheromone components of this species by using GC and GC-MS. Three same components, such as (Z)-11- hexadecenal (Z11-16:Ald), (E)-11-hexdecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z-11-16:OH) were also indentified in Korean population, but there was a little difference in ratios. The ratios of these three compounds of Japanese population and Korean population were 5:1.25:1, and 5:0.96:0.2, respectively. The ratios of Z and E-11-hexadecenal were similar, but the ratio of (Z)-11-hexadecenol was lower compared to Japanese population. In field bioassay, (Z)-11-hexadecenal or (E)-11-hexadecenal alone was not attractive to males, but the mixture of Z11-16:Ald and E11-16:Ald was attractive to males. The most effective ratios of Z11-16:Ald and E11-16:Ald was 5:1. Other ratios such as 1:1 and 1:5 (Z:E) was not attractive to males. The attractiveness of Z:11-16:OH will be tested in near future.

Ecological Characteristic of Glyphodes perspectalis was investigated in laboratory and field condition. Larvae of Glyphodes perspectalis had 6 instars, and color of head and body was black and yellowish green, respectively. Larval periods were 24 days. Egg was flat and round. Color of egg was transparent at an early stage, and became milky later. Color of pupa was yellowish green and became dark brown later. Pupal periods were 10.5 days. Adult of Glyphodes perspectalis emerged twice a year. First emergency was from early June to late June, and second from middle August to early September.