The objective of the present study was to evaluate the potential antidiabetic and antioxidant effect of the ethanol extract from Chrysanthemum cornarium L. var. spatiosum(CSE) against alloxan-induced oxidative stress in pancreatic β-cells, HIT-T15. In this study, the antidiabetic effect of CSE was examined using the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazoliu bromide(MTT) cell proliferation assay, lactate dehydrogenase(LDH) release assay, NAD+/NADH ratio and insulin secretion. To further investigate whether CSE is involved in the antioxidant activity of alloxan-damaged HIT-T15 cells, its antioxidant effect against alloxan-induced oxidative stress was measured in HIT-T15 cells by determining the levels of antioxidant enzymes including superoxide dismutase(SOD), glutathione S-transferase(GST), glutathione reductase(GR) and glutathione peroxidase(GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered NAD+/NADH ratio and insulin secretion in HIT-T15 cells. However, CSE significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular NAD+/NADH ratio and insulin secretion were also significantly increased by 1.7-fold and 1.3-fold, respectively, after treatment with 100 ㎍/㎖ CSE. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while CSE significantly elevated the levels of antioxidant enzymes. These findings suggest that CSE could have a protective effect against cytotoxicity and dysfunction of pancreatic cells in the presence of alloxan-induced oxidative stress.

본 연구는 당에 의해 유도된 HIT-T15를 이용하여 항당뇨 소재로 잘 알려져 있는 오미자 및 뽕잎 추출물의 인슐린 분비능에 미치는 영향에 대해 조사하였다. 오미자 발효주정 추출물과 뽕잎 열수 추출물의 총 폴리페놀함량은 각각 mg/g 및 mg/g이었으며, 총 플라보노이드함량은 mg/g 및 mg/g 이었다. 전자공여능은 뽕잎 열수 추출물이 로 오미자 발효 주정 추출물의 보다 높았으며 오미자 및 뽕잎 추출물의 ORAC은 각각 , TE/g