This study investigated the odor-associated bacterial community in automobile HVAC systems. Through a metagenome analysis, it was found that; Massilia (42.426%), Sphingomonas (28.200%), (10.780%), and Methylobacterium (5.756%) were abundant in the HVAC systems. Massilia can cause the biodegradation of polycyclic aromatic hydrocarbons (PAHs) producing odor in automobiles. Sphingomonas produces volatile halogenated compounds or degrades organic pollutants. Rhodococcus is reported to produce sulfur compounds which give off an odor similar to rotting eggs and cabbages. Methylobacterium is one of the most representative bacteria that causes odor in automobile HVAC systems. The evaporator is considered as the appropriate habitat for microorganisms in automobiles because of its high humidity and organic adsorption. Massilia, Sphingomonas, Rhodococcus, Methylobacterium, Bacillus, Staphylococcus, Arthrobacter, Micrococcus, and Pseudomonas, listed in order from most to least present, were isolated as abundant bacteria in the evaporator of the HVAC systems.