본 연구는 애니메이션 <만약...헬라가 텐 링즈를 발견한다면?>을 사례 로 의상 기호의 의미와 의복 문화의 이데올로기가 내러티브에 미치는 영 향을 분석함으로써 기호가 애니메이션 서사에 미치는 지도적 역할을 탐 구하고자 하였다. 연구는 다양한 문화적 환경에서 의상의 색상, 스타일, 소재가 갖는 부가적 의미와 의상 기호가 갖는 이데올로기를 롤랑 바르트 의 기호학을 통해 분석하고, 애니메이션에서 의상 기호의 서사적 역할과 애니메이션의 주제적 아이디어 전달에 대해 연구하였다. 연구 결과에서 는 애니메이션 작가들은 의상 기호를 활용하여 시각적 서사적 도움을 주 고 있으며, 기호의 통합을 통해 작가가 표현하고자 하는 주제적 아이디 어를 설명하고, 관객이 애니메이션 속 플롯의 전개와 등장인물의 감정 변화를 이해하고 느낄 수 있도록 돕고 있다. 이 연구의 결론은 기호학이 애니메이션 서사와 통문화적 기호 사용에 대한 이야기를 전달하는 데 도 움이 되는 아이디어를 제공하고, 더 나은 애니메이션 작품 창작에 학술 적 지원을 제공한다. 그러나 기호를 의상 층면에만 한정함으로써 애니메 이션 전체의 시각적 의미 전달에 대한 지침으로서 많은 한계가 있다.

Treatment of dextran sodium sulfate(DSS) on HeLa cells results to an enhanced susceptibility to Brucella(B.) abortus infection. An increase in the adherence, invasion and intracellular replication of B. abortus was observed in DSS-treated cells. Furthermore, a marked elevation in the intensity of F-actin fluorescence was also observed in DSS-treated cells compared with untreated B. abortus-infected cells. An upregulation of phagocytic signaling proteins by Western blot analysis demonstrated an apparent activation of ERK, p38α and JNK phosphorylation levels in B. abortus-infected DSS-treated cells compared with the control. Colocalization with LAMP-1 proteins was attenuated in DSS-treated cells upon intracellular trafficking of the pathogen compared with control cells. The results of this study demonstrated consistency with other pathogens. The uptake and intracellular replication of B. abortus is hypothesized to be stimulated by various dextran receptors such as C-type lectins that are involved in phagocytosis which can either be direct phagocytic receptors, modulators of the expression of other receptors or as opsonins leading to an enhanced internalization of B. abortus. The complexity of these interactions thus would warrant further investigation into the role of DSS in the pathogenesis of brucellosis. In summary, we conclude that DSS enhanced adhesion, phagocytosis and intracellular replication of B. abortus in epithelial cells which could lead to suppression of the innate immune system in chronic Brucella infection.

This study examined the effects of tamoxifen at different concentrations and treatment periods on proliferation of a human cervical cancer cell line, HeLa. As in the previous studies, Estrogen did not have an effect on the cellular proliferation at low concentrations but significantly reduced proliferation at high concentration (5 ug/ml) based on MTT assay. Treatment with tamoxifen had similar effects. It did not have any significant effect on the HeLa cell proliferation at low concentrations, but significantly reduced proliferation at high concentration (10 uM). In addition, combined treatment with both estrogen and tamoxifen did not alter the inhibitory effects of either estrogen or tamoxifen on cellular proliferation. The inhibitory effects by tamoxifen on HeLa cell proliferation did not differ among different treatment periods. This suggests that tamoxifen may exert anti- proliferative effects at high concentration but does not have synergistic or antagonistic effects against estrogen on HeLa cell proliferation.

Many studies propose that dysfunction of mitochondrial proton-translocating NADH-ubiquinone oxidoreductase (complex I) is associated with neurodegenerative disorders, such as Parkinson's disease and Huntington's disease. Mammalian mitochondrial proton-translocating NADH-quinone oxidoreductase (complex I) consists of at least 46 different subunits. In contrast, the NDI1 gene of Saccharomyces cerevisiae is a single subunit rotenone-insensitive NADH-quinone oxidoreductase that is located on the matrix side of the inner mitochondrial membrane. With a recombinant adeno-associated virus vector carrying the NDI1 gene (rAAV-NDI1) as the gene delivery method, we were able to attain high transduction efficiencies even in the human epithelial cervical cancer cells that are difficult to transfect by lipofection or calcium phosphate precipitation methods. Using a rAAV-NDI1, we demonstrated that the Ndi1 enzyme is successfully expressed in HeLa cells. The expressed Ndi1 enzyme was recognized to be localized in mitochondria by confocal immunofluorescence microscopic analyses and immunoblotting. Using digitonin-permeabilized cells, it was shown that the NADH oxidase activity of the NDI1-transduced HeLa cells were not affected by rotenone which is inhibitor of complex I, but was inhibited by flavone and antimycin A. The NDI1-transduced cells were able to grow in media containing rotenone. In contrast, control cells that did not receive the NDI1 gene failed to survive. In particular, in the NDI1-transduced cells, the yeast enzyme becomes integrated into the human respiratory chain. It is concluded that the NDI1 gene provides a potentially useful tool for gene therapy of mitochondrial diseases caused by complex I deficiency.

세포에 미치는 염화수은(II)과 이온화 방사선의 영향과 수은 처리 전 후 방사선 조사 시 그 상호 작용에 관해 알아보고자 본 연구를 수행하였다. 염화수은(II)의 독성정도를 알아보기 위하여 사람의 자궁암 세포에 농도별로 염화수은(II)을 처리하였다. 세포의 생존율은 3가지 농도(1,0. 1,0. 0.01 μM)모두에서 유의하게 감소하였으며 이미 0.1 μM에서 약 73%의 생존율이 감소하는 것으로 나타났다. 염화수은(II)과 방