최근 벚나무에 벚나무깍지벌레에 의한 피해가 증가하여 친환경적인 방제제로서 곤충병원성 곰팡이인 Beauveria bassiana ARP14를 검증하였다. 경북 안동시 안동대학교 캠퍼스 내 벚나무에서 벚나무깍지벌레의 감염율을 조사하고, 벚나무의 연령을 추정하여 상관관계를 분석하였다. 곤충병원성 곰팡이의 살충력을 확인하 기 위해 약충의 이동성이 가장 활발한 4월 말부터 5월 중순까지 벚나무깍지벌레에 감염된 가지를 채집하여 23°C 의 항온기에 보관하면서 두 가지 곤충병원성 곰팡이(ARP14, AAD16)를 살포하여 살충제(스피로테트라맛 액상 수화제) 처리구와 살충률을 비교하였다. 그 결과 대조구(물처리)보다 곤충병원성 곰팡이를 살포한 가지에서 약충의 개체 수가 더 감소하였으나, 살충제를 처리한 가지에서 보다는 약충의 수가 많았다. 따라서 가로수로 많이 식재된 벚나무 해충 방제시 일어날 수 있는 사람에 대한 피해를 최소화할 수 있는 친환경 방제제로서 곤충병 원성 곰팡이를 사용할 수 있을 것이다.

Background: In modern society, the use of computers accounts for a large proportion of our daily lives. Although substantial research is being actively conducted on musculoskeletal diseases resulting from computer use, there has been a recent surge in interest in improving the working environment for prevention. Objects: This study aimed to examine the effects of posture correction feedback (PCF) on changes in neck posture and muscle activation during computer typing. Methods: The participants performed a computer typing task in two sessions, each lasting 16 minutes. The participant’s dominant side was photographed and analyzed using ImageJ software to verify neck posture. Surface electromyography (EMG) was used to confirm the participant’s cervical erector spinae (CES) and upper trapezius muscle activities. The EMG signal was analyzed using the percentage of reference voluntary contraction and amplitude probability distribution function (APDF). In the second session, visual and auditory feedback for posture correction was provided if the neck was flexed by more than 15° in the initial position during computer typing. A 20-minute rest period was provided between the two sessions. Results: The neck angle (p = 0.014), CES muscle activity (p = 0.008), and APDF (p = 0.015) showed significant differences depending on the presence of the PCF. Furthermore, significant differences were observed regarding the CES muscle activity (p = 0.001) and APDF (p = 0.002) over time. Conclusion: Our study showed that the feedback system can correct poor posture and reduces unnecessary muscle activation during computer work. The improved neck posture and reduced CES muscle activity observed in this study suggest that neck pain can be prevented. Based on these results, we suggest that the PCF system can be used to prevent neck pain.

This study assessed the utility of netted melon ‘Top Earl’s’ and cantaloupe melon ‘Alex’ as functional fruits by analysing their moisture content, vitreous sugar, folic acid, citric acid, and beta-carotene levels. High-performance liquid chromatography (HPLC) was used to analyse the free sugar, folic acid, citric acid, and beta-carotene levels. The moisture content was not significantly different between ‘Top Earl’s’ and ‘Alex.’ The glucose, sucrose, and fructose contents were three, two, and one-and-a-half fold higher in ‘Alex’ than in ‘Top Earl’s.’ Moreover, citric acid was approximately three times higher in ‘Alex’ than that in Top Earl’s.’ However, the folic acid content was higher in ‘Top ‘Earl’s’ than ‘Alex,’ and the amount was 124 μg / 100 g FW and 112 μg / 100 g FW respectively. ‘Beta-carotene was undetectable in ‘Top Earl’s,’ whereas it was 1000 μg / 100 g FW in ‘Alex.’ β-carotene, a substance that is converted in the body into vitamin A and acts as an antioxidant, is an important component in healthy food. These results suggested that the cantaloupe melon ‘Alex’ has a higher free sugar content and functional ingredients, such as antioxidants, including citric acid and beta carotene, than the netted melon ‘Top Earl’s.’

The purpose of the study is to assess morphological and chromosomal comparison of Mini type Phalanopsis ‘KS Little Gem’ and four domestic market available cultivars (‘Queen Beer’, ‘Tony Pink’, ‘Vaviche’ and ‘Rorens’). ‘KS Little Gem’ had the highest number of leaves (14.9), while the other four types had fewer than ten. The longest leaf length was 21.5㎝ for ‘Tony Pink’, followed by 16.2㎝, 18.0㎝ and 17.5㎝ for ‘Queen Beer’, ‘Rorens’ and ‘Vaviche’ respectively. The length and width of the petals of ‘KS Little Gem’ were 29.5㎜ and 25.6㎜ respectively indicating a round shape flower compared to other cultivars. When the flower lifespan of ‘KS Little Gem’ was compared to four cultivars of the domestic market, it was found that it had a 123-day shelf life, which was twice longer than that of the four cultivars. According to chromosome analysis ‘KS Little Gem’, ‘Rorens’, ‘Tony Pink,' and ‘Vaviche’ were tetraploid (2n = 4x = 76) while ‘Queen Beer’ was diploid (2n = 2x = 38). The estimated DNA content of ‘KS Little Gem’, ‘Rorens’, ‘Tony Pink’ and ‘Vaviche’ had 4918.4, 4794.2, 4705.2 and 4964.3 Mbp respectively, which were roughly double than that of P. cornu-cervi (control, 2n = 2x = 38). However, ‘Queen Beer’, had an estimated DNA content of 2802.2Mbp, similar to that of P. cornu-cervi. The morphological features, genome size and chromosomal data reported in these studies can be used by breeders to create more efficient Phalaenopsis breeding programs.

Quantitative real-time polymerase chain reaction (RTqPCR) is a rapid and precise method of analysis to quantify the level of gene expression and is widely used in the diagnosis of diseases and quantitative analysis of genes. In RT-qPCR analysis, a reference gene (or housekeeping gene) is used for normalization of experimental results. Since this method of analysis detects a small quantity of the product, it is highly sensitive and it is important for the accuracy and reproducibility of the experiment to select a reference gene suitable for gene expression studies. As the expression levels of the reference gene are affected under different conditions, in order to determine the suitability of the housekeeping gene used as the reference gene, it is necessary to verify the expression stability. In the current study, the stability of the expression of 11 housekeeping genes (B2M, SDHA, GAPDH, RPL13, VIM, EEF1A1, HPRT1, GUSB, RPL19, ACTB, and ABL1) was investigated in the tissues of long-tailed chickens (heart, thigh, and breast). Expression stability evaluation was analyzed with four software: BestKeeper, NormFinder, geNorm, and RefFinder. In our study, GAPDH in heart tissue, HPRT1 in thigh tissue, and RPL13 in breast tissue were selected as the most stable reference genes. Evaluation of the expression stability of housekeeping genes can provide important data in gene expression studies by selecting an appropriate reference gene according to various conditions.

An investigation on the habitation of Callipogon relictus Semenov was carried out in Gwangneung forest, Korea and Ussurisky nature reserve, Russia. During the investigation period (2007–2018), a total of 25 emergence holes, 10 pupal chambers, 10 adults, and two larvae of C. relictus were identified in Gwangneung forest. In this presentation, we provide biological information on habitation of C. relictus in Gwangneung forest by comparison and combination of survey results in Ussurisky nature reserve, in 2015 and 2018. In addition, the distribution range of C. relictus is also reviewed in the Korean Peninsula with a new location.

The hawthorn spider mite, Amphitetranychus viennensis (Zacher), is an important tetranychid causing damage on fruittrees and woody ornamental plants. We investigated the effect of a mycoinsecticide BotaniGard® ES at different humiditylevels on the eggs and adults mortality of A. viennensis. In a laboratory condition, egg mortality was significantly influencedby the conidial concentration and RHs. Highest mortality of eggs was observed at 1×108 conidia/ml with 95% RH thanother conditions. In 1×108 conidia/ml, LT50 at 95 and 75% RH were significantly different from 55% RH. In BotaniGard®spray in cherry plants, mites decreased on day 6 at all the concentrations, but the mite populations rebounded exceptin 1×109 conidia/ml where it maintained 0 until 9 days after spraying. It is therefore concluded that B. bassiana hasa potential to be used against A. viennensis

A karyotype analysis of five wild rose species (Rosa helenae, R. mulliganii, R. multiflora, R. rubus, and R. solieana) was performed using bicolor fluorescence in situ hybridization (FISH). All five species were found to be diploid (2n = 2x = 14). The chromosome length in the metaphase stage ranged from 1.29 to 2.05 μm in R. helenae, 3.32 to 6.82 μm in R. mulliganii, 1.58 to 2.24 μm in R. multiflora, 2.05 to 3.46 μm in R. rubus, and 1.62 to 2.46 μm in R. solieana. The chromosomes were either metacentric or submetacentric, with no subtelocentric or telocentric chromosomes being observed. Each p air of 5 S and 4 5S rDNA sites was detected at the proximal region of the long arm and the terminal region of the short arm of chromosome #7, respectively.

Most known species in the Strophariaceae are decomposers and grow on various kind of organic matter. Approximately 18 genera and 1,316 species in the Strophariaceae have been reported worldwide. Through an ongoing survey of indigenous fungi in Korea, 29 specimens belonging to the Strophariaceae were collected from 2012 to 2016. These specimens were identified based on morphological characteristics and molecular analysis of internal transcribed spacer sequences. Fifteen taxa were confirmed, with eight species matching those previously recorded. Seven species in five genera were shown to be new records in Korea: Galerina marginata, Gymnopilus crociphyllus, Gymnopilus picreus, Hebeloma birrus, Hebeloma cavipes, Pholiota multicingulata, and Psilocybe thaizapoteca. In this study, we provide detailed morphological descriptions of these species and investigate their evolutionary relationships by constructing phylogenetic trees.

A diversification of external morphology and genital characteristics among conspecific animal populations that are geographically divided is particularly important to study the context of evolution and it is regarded as the early stage of allopatric speciation. The aim of the present study is to understand the morphological variations of Callipogon relictus Semenov from the three different populations (South Korea, China, Russia) in the Eastern Palaearctic region. A morphometric analysis was performed based on 87 selected external and internal characteristics from 95 specimens. As results of the present study, South Korean specimens shows remarkable morphological differences in genital characteristics compare to Russian ones. In Principal Component Analysis (PCA), South Korean and Russian populations are divided into two groups and the Analysis of Variance (ANOVA) analysis indicate that these two populations have significant differences in 14 morphometric characteristics.

6-Gingerol exerts anti-tumor effects in various cancer cell models. We evaluated the effect of 6-gingerol on the growth of MCF-7 breast cancer cells and MCF-10A breast epithelial cells to determine whether any growth-inhibitory effects found were attributable to apoptosis, and to elucidate the underlying mechanism of action. 6-Gingerol inhibited the viability of both cell lines in a dose- and time-dependent manner; however, the degree of inhibition was greater in MCF-7 than MCF-10A cells. By flow cytometry, induction of dose- and time-dependent apoptosis was found, and the magnitude of apoptosis was also markedly greater in MCF-7 than MCF-10A cells. Expression of caspase-3 and poly (ADP-ribose) polymerase (PARP) was observed in MCF-7 cells treated with 6-gingerol, and further cleavage of PARP occurred in these cells. We suggest that 6-gingerol induces apoptosis in human breast cancer cells mainly by promoting caspase-3 expression and subsequent degradation of PARP.