LC-DAD-ESI/MS를 이용하여 국내 자색벼 품종에 대해 개별 안토시아닌 조성 및 함량을 평가한 결과는 다음과 같다. 1. 자색벼 품종에서 분리된 모든 개별 안토시아닌의 화학구조는 MS fragment 패턴을 확인하여 cyanidin을 base로 한 unknown 화합물 1종을 포함, cyanidin 3,5-diglucoside, cyanidin 3-glucoside, peonidin 3-glucoside의 총 4가지 개별성분이 분리 및 동정되었다. 2. Cyanidin 3-glucoside 및 peonidin 3-glucoside이 주요성분으로 cyanidin 3-glucoside의 경우 90% 이상의 가장 높은 함량 비중을 나타냈으며, 개별성분별 평균 함량은 cyanidin 3-glucoside > peonidin 3-glucoside > cyanidin 3,5-diglucoside > unknown(cyanidin based)의 순으로 나타났다. 3. 흑진주벼의 총 안토시아닌 함량은 408 mg/100 g으로 흑남벼보다 약 6배 정도 높은 함량을 나타내었다.

In this study, baicalin, as a marker substance of Scutellariae Radix, was quantitatively analyzed by a high performance liquid chromatography-photodiode array detector (HPLC-DAD). We identified wogonoside, baicalein, and wogonin in the Scutellariae Radix by a high performance liquid chromatography-electrospray ionization-mass spectrometer (HPLC-ESI-MS). The baicalin was separated on a Xterra C18 column (5 ㎛, 4.6 x 250 ㎜) using mobile phase consisting of 38% acetonitrile in 0.68% phosphoric acid. The baicalin spectrum in the Scutellariae Radix extracts was coincided by comparing with UV-visible spectrum (200-550 ㎚) of baicalin standard in the library. The amount of baicalin in Scutellariae Radix was 10.46%, which is higher than KFDA’s guideline. The marker substances of Scutellariae Radix showed a strong base peak [M]+ in the positive detection mode following as; baicalin (m/z; 271 [MH+-sugar]+, 447 [M+H]+), wogonoside (m/z; 285 [MH+-sugar]+, 461 [M+H]+), baicalein (m/z; 271 [M+H]+), wogonin (m/z; 285 [M+H]+). These results are consistent with the fragment pattern and molecular weight of standard components from literature.

The quality control of natural products is principal key to guarantee the Good Manufacturing Practices (GMP) and Good Clinical Practices (GCP) for the functional food, pharmaceuticals and cosmeceuticals in the industry. In this study, we examined the quantitative analysis of berberine as marker substance of Coptidis Rhizoma by high performance liquid chromatography-photodiode array detector (HPLC-DAD). The HPLC method was validated and met all the requirements for the quality control analysis recommended by FDA and ICH. The berberine was separated on a Xterra C18 column (5 ㎛, 4.6 × 250 ㎜) using mobile phase consisting of distilled water and acetonitrile with KH2PO4 (3.4 g) and Na2SO4 (1.7 g). Calibration curve of berberine has been estimated (y = 42293.47x-41589 with the correlation coefficient 0.9999). The amount of berberine was calculated as 4.25%. And berberastine, palmatine, columbamine, jatrorrhizine, epiberberine, berberine and coptisine in the Coptidis Rhizoma were identified by high performance liquid chromatography - electrospray ionization-mass spectrometer (HPLC-ESI-MS) method.