This study was designed to evaluate the objective meat qualities of Hanwoo longissimus lumborum muscle after a period of long-term storage (40 days) in which conditions similar to those under which the meat would be stored for export to the Hong Kong beef market were simulated. Twelve LL muscles were sampled from animals slaughtered the previous day at a commercial beef export abattoir and assigned to one of three groups. Each group was subjected to a different packaging condition; Shrink film packed (SFP), vacuum packed (VP), or modified atmosphere packed (MAP)(O2/60%, CO2/40%). Objective meat qualities were assessed at day 1, 7, 21 and 40 of storage. Different Packaging conditions had no noticeable influence on cooking loss significantly. However, the moisture content in both the SFP and MAP groups tended to decline in a linear manner with storage periods throughout the 40 days period. Drip loss of MAP (5.68%) group was much higher in the SFP (3.18%) and VP (2.64%) groups at storage day 40. Redness (CIE a*) of meat color responded in a significant and completely different manner to each packaging method. Redness significantly (p<0.05) and continuously increased 17.51 at day 1 to 20.41 at day 40 in VP group, while MAP linearly dropped and ultimately reached 10.6 after 40 days of aging (p<0.05), at which point the meat had a brown color. Tests of Warner-Bratzler shear force (WBSF) indicated that the tenderness levels of the ready-for-export Hanwoo LL muscles were acceptable 7 days postmortem in the SFP and VP groups, however there was no significant difference between each group. Our gathered data suggests that the packaging method selected for export determines how well the objective qualities of the beef hold up, and indicate that VP is likely the most reliable method.
본 연구는 한우 암송아지 비육프로그램 개발을 위하여 약 8개월령 30두(평균 체중 233kg)에게 전 시험기간동안 한우 거세우 육성용 사료(CP 13.65%, TDN 72.7% 건물기 준)를 자유채식 또는, 제한급여(체중의 1.5%, 1.6%/일)를 시킨 후, 24개월령부터 출하 시(32개월령)까지는 자유 채 식시켰다. 본 시험에서 출하 시 체중은 685-702kg 이었으 며, 전 기간 평균 사료 섭취량(7.78–9.05kg/일)과 증체량 (0.58–0.64kg/일)은 자유채식구가 제한급여구보다 많았다 (p<0.05). 한우 미경산 암소의 배최장근 단면적은 평균 99.9cm2였으며, 도체중은 평균 407kg이었다. 조리 감량은 수분함량이 높고 보수력이 낮을수록 많았다(p<0.05). 등심 중 아미노산 함량은 glutamic acid, aspartic acid, lysine, leucine 순으로 높았으며, 지방산 중에는 oleic acid가 55.9 –58.7% 수준으로 자유채식구가 제한급여구보다 높았다 (p<0.05). 따라서 한우암소 비육 프로그램으로서, 한우 거세우 육 성용 TMR을 전 기간(6-32개월령) 동안 자유 채식시키면, 사료효율, 도체 성적 및 등심의 물리·화학적 특성에 있어 서도 우수한 결과를 나타낼 수 있을 것으로 판단된다.
This study evaluated the effects of TMC (trace mineral-fortified microbial culture) supplementation on growth performance, carcass characteristics, and meat quality parameters of Hanwoo steers during the last 4 months of finishing period. The TMC was a combination of 0.4% trace minerals, 20.0% Na-bentonite, and 79.6% feedstuffs, which was inoculated with a mixed microbial culture (Enterobacter ludwigii, Bacillus cereus, B. subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae). Twenty-four steers were blocked by initial BW (634 ± 16 kg) and randomly allocated to one of two treatments (control vs. 3.3% TMC). The effect of TMC supplementation on the growth performance was not significant. There was no incidence of urolithiasis in TMCfed steers. However 3 out 12 steers (25%) fed the control diet were observed to have urinary calculi. The carcass yield and meat quality parameters were not affected by TMC supplementation, however marbling score was increased in TMC-fed steers (P = 0.08). There was no effect of TMC treatment on the chemical composition of longissimus dorsi muscle (LM). The TMC supplementation increased concentrations of manganese (P < 0.01), cobalt (P = 0.02), iron, and copper (P = 0.06) in LM. In conclusion, TMC treatment did not negatively affect growth performance and meat quality parameters, and positively affected the trace minerals profile of LM.
This study tested the association between genotypes of the single nucleotide polymorphism (SNP) marker, rs81437607 and capric acid (FA_C10_0) compositions in longissimus dorsi muscle in pigs. Eighteen fatty acid (FA) compositions were measured in a total of 974 F2 animals among 1,106 F2 progeny produced between Landrace and Jeju Black Pig (JBP). Among FA compositions tested, we identified a cluster of highly significant SNPs for capric acid compositions on 58 Mb position of Sus scrofa chromosome 12 (SSC12) using genome-wide association study (GWAS) with F2 genotypes from SNP panel analysis. GWAS results showed that the rs81437607 was the highest trait-related SNP marker with capric acid levels. Three genotypes (C/C, C/T and T/T) of rs81437607 marker were found in F2 population by further pyrosequencing. Association analysis results showed the significant differences between rs81437607 genotypes and capric acid compositions (P<0.05). The F2 pigs harboring rs81437607 C/C (0.119±0.002%) and C/T (0.116±0.002%) genotypes showed additively higher levels of capric acid content than those of T/T homozygotes (0.109±0.002%) (P=1.30×10-12). These results suggested that the genetic variations of rs81437607 may be helpful to find causative variants and assist as molecular genetic markers for improving the capric acid contents in longissimus dorsi muscle in pigs.