PURPOSES : In this study, an eco-friendly mastic asphalt backfill material is developed to reduce production and construction temperatures by 40 ℃ compared with those recorded when using conventional hot-mix mastic asphalt backfill materials. METHODS : To reduce the production and construction temperatures, SIS polymer modifiers and gum rosin were selected, and gum rosin-modified SIS materials were applied to the mastic asphalt binder mix design. SIS is less viscous than SBS at high temperatures owing to its thermal characteristics, and incorporating gum rosin into SIS causes the latter to exhibit a loose and soft structure. To improve the performance of the mastic asphalt modified with SIS and gum rosin, three different filler mixes, i.e., 100% PMMA, 50% PMMA and 50% calcium carbonate, and 40% PMMA and 60% calcium carbonate were applied. RESULTS : The rosin-modified SIS reduces the viscosity of the developed mastic asphalt binders. In particular, incorporating 3.7% of gum rosin is beneficial to the mastic binder and does not degrade its low-temperature performance. Similarly, using 100% PMMA as a filler improves the performance but results in workability issues at high temperatures. CONCLUSIONS : Rosin-modified SIS and PMMA are promising alternatives for increasing the workability at high temperatures while maintaining the target performance of grade PG82-22 binders if the appropriate ratio of calcium carbonate is mixed with PMMA and an alternative filler comprising calcium carbonate is used.

PURPOSES : In this study, we propose a mini-trench method, which involves using warm mix Guss mastic asphalt as a backfill material and an installation temperature of 160 ℃. The method is verified via a heat transfer analysis of a pavement using the finite element method. METHODS : First, the density, thermal conductivity, and specific heat required for heat transfer analysis were determined based on previous studies. Subsequently, the boundary conditions for convection and radiation to perform the heat transfer analysis were determined. The pavement temperature, which is the initial condition of the analysis, was determined based on the summer pavement temperature distribution using the temperature prediction program of the Korean pavement Research Program. Heat transfer analysis was performed by determining the temperature of the backfill material based on 160 °C and 200 °C for the heat load temperatures. The temperature change was observed on the backfill surface, and the temperature change of the conduit was observed directly. RESULTS : When the pavement surface temperature for traffic opening is 50 °C, the backfill thickness ranges from 50 to 250 mm, the warm mix Guss mastic asphalt requires 2 h to 5 h, 15 min until traffic opening, and the hot mix Guss mastic asphalt requires 2 h, 30 min to 6 h, 40 min until traffic opening. The limit temperature of the conduit evaluated based on KS C 8454 shows that the warm mix Guss mastic asphalt does not satisfy the standard when the backfill concrete cover is 50 mm thick, whereas the hot mix Guss mastic asphalt does not satisfy the standard when the concrete cover is 50 and 100 mm thick. CONCLUSIONS : The backfill depth of the mini-trench using warm mix Guss mastic asphalt as a backfill material should be less than 100 mm, considering the traffic opening time. Meanwhile, the thickness of the backfill concrete should be 100 mm or less.

PURPOSES : The purpose of this study was to evaluate newly developed Guss mastic asphalt with polymer modifier as a elastomer and a plastomer in to polymer content. METHODS : As polymer modifiers, 10%, 20% of elastomer and 10%, 20% of plastomer, and 10% of elastomer and 10% of plastomer are all added to the binder, and the physical properties of the Guss mastic asphalt mixture with these binders are changed. The properties of mixtures with workability, penetration depth and dynamic stability were compared with the existing Guss mastic asphalt mixture. RESULTS : When using the elastomer and the plastomer, the dynamic stability of the Guss mastic asphalt mixture was improved compared to the conventional asphalt mixture, and when the amount of the elastomer was 20%, the workability was reduced. In addition, when 10% of the elastomer and 10% of the plastomer were used, the workability was not significantly deteriorated and the dynamic stability was increased. CONCLUSIONS : In order to improve the dynamic stability of the Guss mastic asphalt mixture using the polymer-modified binder, it is effective to use an elastomer and a plaststomer.

PURPOSES : The purpose of this study was to evaluate the newly developed Guss mastic asphalt mixtures, called EQ-mastic asphalt mixtures, which contain melted additives for decreasing cooking time. METHODS : A series of experiments were performed to investigate the effectiveness of the melted additives in EQ-mastic asphalt mixtures. Both the existing Guss mastic asphalt mixture and the EQ-mastic asphalt mixture were produced with the same amounts of asphalt binders, aggregates, and fillers, but the existing Guss mastic asphalt mixture contained 3% Trinidad lake asphalt (TLA). The EQ-mastic asphalt mixture contained 3% of additives, including TLA and polyolefin. The physical material performances of both mastic asphalt mixtures were obtained by conducting the Luer fluidity test, penetration test, dynamic stability test, and low-temperature bending test. The results of the tests for the existing Guss mastic and EQ-mastic asphalt mixtures were compared. RESULTS : The fluidity, penetration, dynamic stability, and low-temperature bending strains of both the existing Guss mastic and EQmastic asphalt mixtures all satisfied the standard values provided in the production and construction guides of the Guss mastic asphalt pavement. CONCLUSIONS : When melted additives containing polyolefin are used in the production of Guss mastic asphalt mixtures, the cooking time decreases, so that the corresponding energy consumption and asphalt fume amount can be reduced. Therefore, an EQ-mastic asphalt mixture is proposed for use as an eco-friendly pavement material.

In this paper, first the aging level of Stone Mastic Asphalt (SMA): one of the widely applied asphalt mixture types for highway construction in South Korea, was analysed then those aging effects on various performance characteristics were studied. Then, a suitable methodology for improving performance on real asphalt pavement construction site was recommended. To fulfil the objective, Gel-Permeation Chromatography (GPC) experimental work was performed on various aged SMA mixtures by measuring Large Molecular Size (LMS) then the Absolute Viscosity (AV) value was predicted based on the findings in the previous step. As results, it was found that types of performance change on aged asphalt binders could be estimated by computed Estimated Absolute Viscosity (EAV) values. It also should be mentioned that the performances of tested SMA mixture presented negative trend after aging effect increases; even though the performance deterioration level of SMA is lower than that of regular Dense Grade Asphalt (DGA) mixture, which means proper reactions are recommended to keep its quality. Moreover, better resistance against aging effect was found by applying Hydrated-Lime (HL) or Low Density Poly-Ethylene (LDPE) compared to any other additives on asphalt mixtures. A unique Aging Quantity (AQ) model for SMA mixtures was developed by using two factors: collected aging time data set from field (and/or laboratory) and AV values based on different temperature conditions. The Predicted Absolute Viscosity (PAV) on SMA mixtures was computed by using the introduced AQ model then the aging level of asphalt binder was estimated as a final step. Additionally, five performance characteristics of asphalt binder: Dynamic Shear Rheometer(DSR) high temperature limit, Bending Beam Rheometer (BBR) low temperature limit, G*/sinδ, Creep stiffness, and m-value, were analysed. The value of AV showed the best performance for predicting and representing aging level. Finally, the aging level of given asphalt mixtures in the field can be easily predicted by choosing one of three approaches presented in this research. It can be concluded that the performance of asphalt pavement can be increased by selecting proper materials and performance prediction methodologies introduced in this study. However, only limited number of specimens were considered in this study due to limit of raw materials and laboratory equipment condition. Therefore, extensive experimental works with various types of asphalt materials are recommended for strengthen findings in this thesis as a future research.

In the present study, we evaluated the effect of CGM on osteogenic differentiation of cultured osteoblasts, and determined whether combination treatment with LLLT had synergistic effects on osteogenic differentiation. The results indicated that CGM promoted proliferation, differentiation, and mineralization of osteoblasts at the threshold concentration of 10 μg/ml; whereas, CGM showed cytotoxic properties at concentrations above 100 μg/ml. ALP activity and mineralization were increased at concentrations above 10 μg/ml . CGM in concentrations up to 10 μg/ml also increased the expression of osteoblast-activated factors including type I collagen, BMP-2, RUNX2, and Osterix. The CGM (50 μg/ml) and LLLT (80 mW for 15 sec) combination treatment group showed the highest proliferation levels, ALP activity, and mineralization ratios. The combination treatment also increased the levels of phosphorylated forms of p38, ATF2, PKD, ERK, and JNK. In addition, the osteoblast differentiation factors including type I collagen, BMP-2, RUNX2, and Osterix protein levels were clearly increased in the combination treatment group. These results suggested that the combination treatment of CGM and LLLT has synergistic effects on the differentiation and mineralization of osteoblastic cells.

Chios Gum Mastic (CGM) is a natural resin extracted from the leaves of Pistacia lentiscus, a plant endemic to the Greek island of Chios. It has been used by traditional healers, and it has antibacterial, antifungal properties, and therapeutic benefits for the skin. The CGM reduces the formation of dental plaque and bacterial growth in oral saliva, and recent studies have demonstrated the role of antioxidant activity of CGM. Although CGM has been widely investigated, its protective effect against oxidative-damage to keratinocytes, as well as the relationship between CGM and autophagy, has not been investigated. The aim of this study was to assess the protective effect of CGM against H2O2-induced oxidative stress and to evaluate the autophagic features induced by CGM in human keratinocytes. The pretreatment with CGM significantly reduced apoptosis in H2O2-exposed HaCaT cells. It promoted the degradation of caspase-3, caspase-8, and caspase-9; and it induced the formation of the processed PARP. The treatment with CGM caused an increase in vesicle formation compared to control group. The level of p62 was reduced and the conversion of LC3-I to LC3-II was increased in CGM treated HaCaT cells. Also, the treatment with CGM increased cleavage of ATG5-ATG12 complex. In summary, CGM helps the cells to survive under stressful conditions by preventing apoptosis and enhancing autophagy. Besides, the present investigation provides evidence to support the antioxidant potential of CGM in vitro and opens up a new horizon for future experiments.

We investigated the synergistic apoptotic effects of co- treatments with Chios gum mastic (CGM) and eugenol on G361 human melanoma cells. An MTT assay was cond-ucted to investigate whether this co-treatment efficiently reduces the viability of G361 cells compared with each single treatment. The induction and augmentation of apop-tosis were confirmed by DNA electrophoresis, Hoechst stai-ning, and analyses of DNA hypoploidy. Western blot ana-lysis and immunofluorescent staining were also performed to evaluate expression and translocation of apoptosis- related proteins following CGM and eugenol co-treatment. Proteasome activity and mitochondrial membrane potential (MMP) changes were also assayed.The results indicated that the co-treatment of CGM and eugenol induces multiple pa-thways and processes associated with an apoptotic response in G361 cells. These include nuclear condensation, DNA fragmentation, a reduction in MMP and proteasome acti-vity, an increase of Bax and decrease of Bcl-2, a decreased DNA content, cytochrome c release into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, and the activation of caspase-9, caspase-7, caspase-3, PARP and DFF45 (ICAD). In contrast, separate treatments of 40 µg/ml CGM or 300 µM eugenol for 24 hours did not induce apoptosis. Our present data thus suggest that a combination therapy of CGM and eugenol is a potential treatment strategy for human melanoma.

Chios gum mastic (CGM) is produced from Pistiacia lentiscus L var chia, which grows only on Chios Island in Greece. CGM is a kind of resin extracted from the stem and leaves, has been used for many centuries in many Mediterranean countries as a dietary supplement and folk medicine for stomach and duodenal ulcers. CGM is known to induce cell cycle arrest and apoptosis in some cancer cells. This study was undertaken to investigate the alteration of the cell cycle and induction of apoptosis following CGM treatment of HL-60 cells. The viability of the HL-60 cells was assessed using the MTT assay. Hoechst staining and DNA electrophoresis were employed to detect HL-60 cells undergoing apoptosis. Western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses were also employed. CGM treatment of HL-60 cells was found to result in a dose- and time-dependent decrease in cell viability and apoptotic cell death. Tested HL-60 cells showed a variety of apoptotic manifestations and induced the downregulation of G1 cell cycle-related proteins. Taken collectively, our present findings demonstrate that CGM strongly induces G1 cell cycle arrest via the modulation of cell cycle-related proteins, and also apoptosis via proteasome, mitochondrial and caspase cascades in HL-60 cells. Hence, we provide evidence that a natural product, CGM could be considered as a novel therapeutic for human leukemia.

Chios gum mastic (CGM) is a resin produced from the stem and leaves of Pistiacia lentiscus L var chia, a plant which grows only on Chios Island in Greece. CGM has been used for many centuries as a dietary supplement and folk medicine for stomach and duodenal ulcers in many Mediterranean countries and is known also to induce cell cycle arrest and apoptosis in some cancer cells. In this study, we further investigated the induction and mechanisms underlying the apoptotic response to CGM treatment in the SCC25 human tongue squamous cell carcinoma cell line. The viability of SCC25 cells, human normal keratinocytes (HaCaT cells) and human gingival fibroblasts (HGF-1 cells), and the growth inhibition of SCC25 cells were assessed by MTT assay and clonogenic assay, respectively. Staining with Hoechst and hemacolor dyes and TUNEL assays were employed to detect SCC25 cells undergoing apoptosis. SCC25 cells were treated with CGM, and this was followed by western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses. CGM treatment of SCC25 cells was found to result in a time- and dosedependent decrease in cell viability, a dose-dependent inhibition of cell growth, and apoptotic cell death. Interestingly, CGM showed a remarkable level of cytotoxicity in SCC25 cells but not in normal cells. Tested SCC25 cells also showed several lines of apoptotic manifestation. Taken together, our present findings demonstrate that CGM strongly inhibits cell proliferation by modulating the expression of G1 cell cycle-related proteins and induces apoptosis via the proteasome, mitochondria and caspase cascades in SCC25 cells.