To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 μg/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.

꽃사과 열매의 anthocyanin색소를 식용으로 개발하기 위하여 에탄올 추출하여 광선, 온도, 당류, 유기산, 금속이온 및 pH에 의한 영향을 검토하였다. 본 색소는 광선에 안정하여 20일 까지도 60% 이상의 안정성을 나타내었고 광선 차단용 포장재를 사용 시 상당한 안정화 효과를 나타내었다. 본 색소는 열에 안정하여 115℃에서 10분까지도 67% 이상의 안정성을 나타내었다. 당에 의해서는 영향을 받지 않았으며, 유기산에 의해서는 색소의 농색화 효과가 높았다. 금속이온에 대해서는 Na^+, K^+, Mg^2+, Ca^2+ 및 Mn^2+ 모두 20일까지 안정하였고, Cu^2+에 의해서는 심각하게 저해되었고 나타내었으며 Al^3+에 의해서는 자색으로 전환되었다. pH가 낮을수록 색소의 농색화 효과가 높았다. 이상의 결과로 볼 때 본 색소는 우수한 천연 적색색소자원이다.