The mineral content of Tricholoma matsutake was evaluated for comparison of mineral contents according to the area of cultivation. Ten domestic and thirty Chinese (10 Yanji, 10 Yunnan and 10 Tibet) T. matsutake specimens were assessed using an atomic absorption spectrophotometer (AAS) and inductively coupled plasma mass spectrometer (ICP-MS). The Na, Mg, K, and Ca contents of domestic T. matsutake were 128.12±85.25 mg/kg, 218.52±105.35 mg/kg, 7,534.58±2,691.52 mg/kg, and 17.69±7.14 mg/kg, respectively, while those of Yanji T. matsutake were 124.89±57.24 mg/kg, 64.07±27.52 mg/kg, 1,439.18±311.04 mg/kg, and 10.88±4.52 mg/kg, respectively. The Na, Mg, K, and Ca contents of Yunnan T. matsutake were 90.78±23.23 mg/kg, 77.40±28.36 mg/kg, 1,446.29 ±126.33 mg/kg, and 28.42±5.18 mg/kg respectively, while those of Tibet T. matsutake were 143.50±41.54 mg/kg, 124.64±50.18 mg/kg, 3,530.95±2,714.99 mg/kg, and 21.05±8.71 mg/kg, respectively. The Cu contents of domestic, Yanji, Yunnan, and Tibet T. matsutake were 105.43±32.97 mg/kg, 19.92±8.95 mg/kg, 54.51±16.91 mg/kg, and 64.80±23.01 mg/kg, respectively. Both domestic and Chinese T. matsutake samples showed significantly different K, Mg, and Cu levels in this study. Therefore, a comparative evaluation of the K, Mg, and Cu contents of multiple domestic and Chinese T. matsutake varieties is needed to determine the appropriate area of cultivation in the future.

A study was conducted to determine the effects of the cattle manure (CM) application on the botanical composition and micro-mineral contents (Fe, Mn, Cu, Zn) of grazing pasture at the experimental field of Livestock Division, Subtropical Animal Experiment Station, National Institute of Animal Science from year 2003 to 2005. The experiment was arranged in a randomized complete block design with three replications. The treatment consisted of T1: 100% chemical fertilizer (CF 100%), T2: 50% CF +50% CM, T3: 25% CF +75% CM, T4: 100% cattle manure (CM 100%), T5: 100% CM (1st yr.)+ 100% CF (2nd yr.) + 100% CM (3rd yr.), T6: 100% CM (1st yr.)+ 100% CF (2nd yr.)+ 100% CF (3rd yr.). The botanical composition of grassland for grass, legumes, and weeds showed that the rate of legumes was increased in all treatments. The weeds rate in T4 was the highest in comparison to the other treatments. For micro-mineral contents T5 showed the highest average Fe contents of 262.08 ppm and T1 showed the lowest (199.20 ppm). Mn contents was the highest in T1 among the other treatments. Zn contents was the highest in T3 as compared with other treatments. Cu contents was the highest in T6 as compared with other treatments. The results of this experiments indicated that micro-mineral contents of change was effect of legumes increased than treatment