Purpose : This study measured obiectively the accommodative responses stimulated by push-up method and minus lens method with the NVision-K 5001 open-field autorefractor, and compared the results with previously published data. Methods : Forty-five subiects aged 19 to 29 years (mean 22. 7±2.4 years) participated in this study. Three conditions were used to stimulate accommodation and accommodative responses were measured objectively by NVision-K 5001 : (1) push-up test under binocular viewing condition ; the target was placed at vergence ranging from 0-50 in 1D steps. (2) push-up test under monocular viewing condition ; the target was placed at vergence ranging from 1-50 in 1D steps. (3) minus lens test under monocular viewing condition ; a distant(6 m) target viewed through increasing powered minus lenses from 1-50 in 10 steps. Trial lens calibration procedures were also performed to assess the accuracy of the NVision-K 5001. Results : The calib1·ation of NVision-1< 5001 wilh trial lenses showed linear regression equation which was close to the idea! 1 : 1 line(y = -1.005x + 0.060, r2 = 0.999. p < 0.001). The accommodative responses measured with the NVision-K 5001 for the push-up and minus lens- stimulated accommodation progressively lagged behind the increasing acconunodative stimulus. 8oth push-up methods under monocular and binocular viewing conditions showed significanlly higher acconunodative responses than minus lens method(p < 0.001). Push-up method under binocular viewing condition demonstrated slightly higher accommodative responses compared with monocular condition, but the difference was not significant(p = 0.958). Conclusion : The objective accommodative responses stimulated by push-up and minus lens method and measured with open-field autorefractor showed good agreement with previously published data.