This study was performed to develop and evaluate beverage prepared with optimally roasted seoritae to maximize the isoflavone content and antioxidant activities of the beverage. Isoflavone content was maximized at the roasting temperature of 110℃ for 20 min. Both DPPH radical scavenging activity and ABTS radical scavenging activity along with total polyphenol content were highest when seoritae was roasted at 110℃ for 20 min. Western blotting was used to determine the level of nuclear factor of activated T-cells 1(NFATc1) involved in controlling osteoclast differentiation. The results showed that NFATc1 had a concentration-dependent inhibitory effect when the RoS110 (roasted seoritae at 110℃ for 20 min) samples were processed at varying concentrations (10, 50, and 100 μg/mL). Tea samples were prepared from optimally roasted seoritae by varying brewing times (5~90 min) at 65℃, and tea brewed for 60 min had the highest preference with 65℃ as the preferred temperature for drinking.
본 연구에서는 서리태 발효추출물의 모발보호효과를 확인하기 위한 연구를 수행하였다. 화학적 산화를 통해 손상된 모발을 준비한 뒤 서리태 발효추출물을 처리하였을 때 모발의 형태학적 특성, 인장강도, 구성성분의 변화를 분석하여 비교하였다. 그 결과 모발에 산화제를 처리하였을 때, 표피의 큐티클 층이 손상되고 모발의 인 장강도가 14.32 ± 0.83 g/cm2에서 12.32 ± 0.79 g/cm2로 감소되었음을 확인하였다. FT-IR 분석결과 산화 제를 처리한 모발은 버진 헤어에 비하여 1,077, 1,041, 801 cm-1에 해당하는 피크가 증가하였으며, 이를 통해 케라틴 단백질 간의 이황화 결합에 필수적인 시스테인이 산화되는 것을 확인하였다. 반면, 손상된 모발에 서리태 발효추출물을 처리한 경우에는 표피의 큐티클 층의 틈이 메워지고, 인장강도가 14.27 ± 0.96 g/cm2로 회복되 었으며, 모발의 성분 중 시스테인의 산화물 비율이 감소하는 것으로 분석되었다. 이러한 결과들을 통해 발효 서 리태 추출물은 산화제에 의해 손상된 모발의 보호 소재로 연구될 가치가 있는 것으로 기대된다.
In order to investigate the utilization of the Omija (Schizandra chinensis Baillon) extract as a natural coagulant for manufacturing soybean curd, the quality characteristics of white (Baktae) and black (Seoritae) soybean curds, coagulated by the Omija extract or MgCl2, were evaluated. Crude protein (6.14±0.30 and 6.25±0.18%, respectively) and crude lipid (10.86±1.74 and 11.29±1.69%, respectively) contents of white and black soybean curds coagulated using the Omija extract were higher than those coagulated using MgCl2. Black soybean curds coagulated using the Omija extract showed higher L, a, and b values than those using MgCl2. The most abundant amino acid in white and black soybean curds coagulated using the Omija extract was arginine (3.74 and 3.71 mg/100 g, dry basis, respectively). The amounts of Ca, K, Mg, and Na were the highest in both soybean curds prepared with the Omija extract. The sensory evaluation (color, flavor, taste, texture, and overall preference) showed that white and black soybean curds coagulated using the Omija extract were more preferred than those produced using MgCl2. The results suggested that using the Omija extract as a natural coagulant agent could improve the quality and sensory characteristics of soybean curds.