For people who have a food allergy the only way to manage the allergy is to avoid the food allergen. The mackerel is one of the major food allergens, but no immunoassay for the rapid and simple detection of mackerel has been reported. The objectives of this study are to develop and characterize monoclonal antibodies (MAbs) specific to mackerel using thermal stable-soluble proteins (TSSP) as an immunogen and to characterize the MAbs by indirect enzyme-linked immunosorbent assay (iELISA). The mice immunized with mackerel TSSP and showing high titer were used for cell fusion and cloning. The characterization of MAbs produced from hybridoma cells obtained was confirmed by indirect ELISA and western blot. Four MAbs were confirmed to be specific to mackerel without crossreaction to other marine products and livestock products in the both methods. The iELISA and western blot based on the MAbs can sensitively detect 1% mackerel protein in other marine products. These results support that immunochemical methods based on the MAb produced could be used as rapid means to detect low levels of mackerel and to identify mackerel adulterated in food.

This study was conducted to determine the replacement effect of spray dried plasma protein (SDPP) with dried porcine solubles (DPS) in weaning pigs. An ileal digestibility trial, at first, was conducted to formulate the experimental diets with SDPP or DPS. Six piglets (21-d old and 6.12kg BW) with T -cannula in the terminal ileum were used. In a feeding trial, 180 pigs (21-d old and 5.98kg BW) were alloted in a completely randomized block design. Treatments were T1 (SDPP 5%, DPS 0%), T2 (SDPP 5%, DPS 2.5%), T3 (SDPP 2.5%, DPS 0%), and T4 (SDPP 2.5%, DPS 2.5%) for phase I, and T1 (SDPP 3%, DPS 0%), T2 (SDPP 3%, DPS 1.5%), T3 (SDPP 1.5%, DPS 0%), and T4 (SDPP 1.5%, DPS 1.5%) for phase Ⅱ. Phase Ⅰ(0～1 week) diet was formulated to contain 3,300ME kcal/kg and 1.25% digestible lysine, and phase Ⅱ (2～3 week) diet contained 3,320ME kcal/kg and 1.10% digestible lysine. Chemical pompositions of the protein sources were higher in SDPP than DPS: CP (81.60 vs. 56.01), lysine (5.95 vs. 3.36), and methionine (1.85 vs. 1.22). Apparent ileal digestibilities of arginine, isoleucine, leucine, lysine, methionine, threonine and phenylalanine were higher in DPS than SDPP (p<0.05). The apparent ileal digestibility of essential amino acids (average) was also higher in DPS than SDPP (p<0.05). There were no significant differences (p>0.05) in ADG and ADFI of piglets during phase Ⅰ (0～1 week) and Ⅱ (2～3 week) among treatments. However, during phase Ⅱ, T2 showed better FCR than T1 (p<0.05). During the overall period, there was no significant difference in growth performance among treatments. When DPS was partially replaced for SDPP, the diet cost was significantly reduced (p<0.05) in terms of cost/kg of body weight. In summary, the digestibility of DPS was exellent, and it would be concluded that DPS can be partially replaced in the young pigs' diet containing SDPP in order to reduce diet cost in weaning pigs.

Orchardgrass 및 white clover의 단파 및 혼파 재배조건에서 미량요소 Fe, Mn, Cu, Zn, Mo및 B의 조합시비가 목초의 생육, 개화, 수량, 양분 함량 및 식생구성비율 등에 미치는 영향을 구명하였다. 다량요소 양분을 동일 량 시비한 조건에서 7 수준의 미량요소 조합시비는 ; 대조구, ; Fe, ; Fe+Mn, ; Fe+Mn＋Cu, ; Fe＋Mn＋Cu＋Zn, ; Fe＋Mn＋Cu＋Zn＋Mo 및 ; Fe＋Mn＋Cu＋Zn＋Mo＋B로

Orchardgrass 및 white clover의 단파 및 혼파재배에서 미량요소 붕소(B)의 시비수준별 목초의 생육상, 뿌리/근류 형성, 수량성, 영양성분/무기양분의 함량 및 초종간 경합지수 등에 미치는 영향을 구명하였다. 처리내용은 다양 및 미량요소 시비를 동일하게 한 조건에서 5 수준의 붕소 처리: 1) ; 0.0, 2) ; 0.2, 3) ; 2.0. 4) ; 6.0, 5) B; 15.0me B/pot로 glasshouse에서 시험으로 수행하였다.

단백질구성 변화를 여러가지 온도조건에서 Japonica형인 진흥, Indica Japonica교잡품종인 통일, 밀양 2003, 영남조생, 유신등을 공시하여 'Growth Cabinet'를 사용하여 검토 하였던바 다음과 같은 결과를 얻었다. 1. 저온하에서 엽록소함양감소는 Japonica형 품종보다 Indica형 품종에서 심하였다. 2. 단백질구성비(저분자가용성단백질에 대한 고분자가용성단백질의 비)는 고온하에서 높았고, 저온하에서 낮았다. 3. 품종간 단백질구성비는 저온하에서 Japonica형 품종보다 Indica형 품종이 낮았다. 4. 엽신의 엽록소함양과 가용성단백질구성비와는 정의 유의 상관관계가 인정되었다.관계가 인정되었다.